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Pla2g4a

Manufactured by Santa Cruz Biotechnology
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PLA2G4A is a gene that encodes the cytosolic phospholipase A2 enzyme. This enzyme plays a role in the release of arachidonic acid from phospholipids, which can then be used in the production of eicosanoids, such as prostaglandins and leukotrienes. The PLA2G4A gene is involved in various cellular processes, including inflammation, cell signaling, and lipid metabolism.

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Lab products found in correlation

Atf6α, by Abcam (1 mentions) β actin, by Abcam (1 mentions) Grp78, by Cell Signaling Technology (1 mentions) Slc7a11, by Cell Signaling Technology (1 mentions) Ire1a, by Cell Signaling Technology (1 mentions) P ampk, by Cell Signaling Technology (1 mentions) Ncoa4, by Cell Signaling Technology (1 mentions) Lc3a b, by Cell Signaling Technology (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Hcc827, by American Type Culture Collection (1 mentions) Gapdh 10494 1 ap, by Proteintech (1 mentions)

3 protocols using pla2g4a

1

Western Blot Analysis of Cellular Proteins

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Western blot analysis was performed as previously described.21 Primary antibodies for ATF6α (Abcam), β‐actin (Abcam), GPX4 (Abcam), PLA2G4A (Santa Cruz Biotechnology), and secondary antibody (Abcam) were applied according to the manufacturer's instructions.
Zhao R., Lv Y., Feng T., Zhang R., Ge L., Pan J., Han B., Song G, & Wang L. (2022). ATF6α promotes prostate cancer progression by enhancing PLA2G4A‐mediated arachidonic acid metabolism and protecting tumor cells against ferroptosis. The Prostate, 82(5), 617-629.
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2

Investigating Lung Cancer Cell Lines

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The human lung cancer cell lines A549, H157, HCC827, and PC9 were obtained from the American Type Culture Collection and were genotyped and authenticated before experiments. Cells were cultured in RPMI-1640 medium (HyClone) supplemented with 10% fetal bovine serum (ZETA) at 37°C in a humidified incubator with 5% CO2. Purified MA (>98%) (#sc-200733) was purchased from Santa Cruz. The 20 mM stock solution was made in DMSO. N-acetyl-l-cysteine (NAC) (#HY-B0215), necrostatin-1 (#HY-15760), Z-VAD-FMK (#HY-16658B), liproxstatin-1 (#HY-12726), erastin (#HY-15763) and osimertinib (#HY-15772) were purchased from MCE. The antibodies used were as follows: p-ERK (#4370), ERK (#4695), p-AMPK (#2535), AMPK (#5832), GPX4 (#52455), SLC7A11 (#12691), NRF2 (#12721), NCOA4 (#66849), FTH1 (#4393), PERK (#5683), IRE1a (#3294), LC3A/B (#12741) and GRP78 (#3177) were purchased from Cell Signaling Technology. BCL2 (#12789-1-AP), KRAS (#12063-1-AP) and GAPDH (#10494-1-AP) were purchased from Proteintech. PLA2G4A (#sc-454) was purchased from Santa Cruz.
Ni Y., Liu J., Zeng L., Yang Y., Liu L., Yao M., Chai L., Zhang L., Li Y., Zhang L, & Li W. (2023). Natural product manoalide promotes EGFR-TKI sensitivity of lung cancer cells by KRAS-ERK pathway and mitochondrial Ca2+ overload-induced ferroptosis. Frontiers in Pharmacology, 13, 1109822.
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3

Quantification of PLA2G4A Expression

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PLA2G4A protein expression was verified in ovarian tissue samples by Western blot, and β-actin was used as a loading control.
Protein aliquots (50 μg) from ovaries treated with VCD, VCD and ginseng or with no treatment was loaded onto a 12% sodium dodecyl sulfate gel, and electrotransferred to a PVDF membrane. Membranes were blocked in 10% nonfat dried milk. The membrane was incubated with rat anticytosolic phospholipase A2 (PLA2G4A; Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1 : 1000) at 4°C for 12 h. Blots were incubated with HRP-conjugated IgG (1 : 1000; Sigma-Aldrich, St. Louis, MO, USA) at room temperature for 1 h. The PLA2G4A protein was detected by chemiluminescence (Pierce, Rockford, IL, USA).
Zhu L., Li J., Xing N., Han D., Kuang H, & Ge P. (2015). American Ginseng Regulates Gene Expression to Protect against Premature Ovarian Failure in Rats. BioMed Research International, 2015, 767124.
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