Ultrasensitive sp rabbit ihc kit

Immunized and control mice were anesthetized with ether and infected intranasally (i.n.) with 30 μl of 5 × 10 5 IFUs C. psittaci in a sucrose-phosphate-glutamic acid (SPG) buffer 2 weeks after the last immunization to evaluate the protective efficiencies [18, (link)19] (link). All mice were weighed before and 3, 7, and 10 days after challenge. The remaining 15 mice in each group were euthanized, and ten infected lungs from each group were dissected and homogenized in Hanks ' solution (Hyclone, Logan, Utah, USA) 10 days after infection, and one-half of the lung homogenates were re-suspended in SPG buffer for cytokine level determinations. The other homogenates were re-suspended in SPG buffer containing gentamicin (10 μg/ml), vancomycin (100 μg/ml), and streptomycin (100 μg/ml) for C. psittaci titer measurements. Lung homogenates from each mouse were centrifuged (20 min, 4000 rpm), and serial dilutions of the supernatants were inoculated onto HeLa 229 cell monolayers. The number of inclusion was counting using a fluorescence microscopy assay. The lungs of five mice from each group were fixed in 10% formaldehyde and embedded in paraffin for histopathological evaluation with hematoxylin-eosin (H&E) and an UltraSensitive™SP (Rabbit) IHC Kit (Maixin, China).