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Si sirt3

Manufactured by Santa Cruz Biotechnology
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Si-Sirt3 is a laboratory reagent that inhibits the activity of the Sirt3 protein, which is involved in the regulation of cellular metabolism and mitochondrial function. The product is intended for research use only and its core function is to provide a tool for studying the role of Sirt3 in various biological processes.

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Lab products found in correlation

Si pgc1α, by Santa Cruz Biotechnology (1 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions) Si sirt1, by Santa Cruz Biotechnology (1 mentions)

2 protocols using si sirt3

1

Silencing Sirt3 and PGC1α in OGD/BDB

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To knockdown the expression of Sirt3 and PGC1α protein, Si-Sirt3 (sc-61556) and Si-PGC1α (sc-72151) were obtained from Santa Cruz. Negative control siRNA Si-control (sc-37007) was used as control. The siRNA molecules were transfected using Lipofectamine RNAiMax reagent (Invitrogen, CA, United States) in Opti-MEM medium according to the manufacturer’s instructions. After incubation for 48 h, cells were treated with OGD and/or BDB.
Qin S.G., Tian H.Y., Wei J., Han Z.H., Zhang M.J., Hao G.H., Liu X, & Pan L.F. (2018). 3-Bromo-4,5-Dihydroxybenzaldehyde Protects Against Myocardial Ischemia and Reperfusion Injury Through the Akt-PGC1α-Sirt3 Pathway. Frontiers in Pharmacology, 9, 722.
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2

Optimizing siRNA-mediated gene silencing

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ON-TARGET plus SMART pool small-interfering RNAs (siRNAs) for si-Controls were obtained from Dharmacon Research (Lafayette, CO, USA). si-SIRT1, si-PGC-1a, and si-SIRT3 were purchased from Santa Cruz. Transient transfection was performed using INTERFERin siRNA transfection reagent (jetPRIME®, polyplus transfection, UK) according to the manufacturer’s guide.
Ma C.H., Chou W.C., Wu C.H., Jou I.M., Tu Y.K., Hsieh P.L, & Tsai K.L. (2021). Ginsenoside Rg3 Attenuates TNF-α-Induced Damage in Chondrocytes through Regulating SIRT1-Mediated Anti-Apoptotic and Anti-Inflammatory Mechanisms. Antioxidants, 10(12), 1972.
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