YUMM1.7 (SCC227) and YUMMER (SCC243) lines from Millipore Sigma (Burlington, MA, USA); YUMM1.G1 (CRL-3363), YUMM3.3 (CRL-3365), YUMM4.1 (CRL-3366), YUMM5.2 (CRL-3367) from ATCC (Manassas, VA, USA) were maintained in DMEM-F12 medium (ATCC (Manassas, VA, USA), 30-2006) supplemented with 10% FBS (Gibco (Waltham, MA, USA) #10437-028), 1% NEAA (Gibco, 11140-50), and 1% pen-Strep (ThermoFisher (Waltham, MA, USA), 15140122). For generating Vem–resistant lines, cells were seeded at ~20% confluence and allowed to adhere overnight, then cultured in medium containing 5 µM Vem (AmBeed (Arlington Heights, IL, USA), A116840) and refreshed every 2–3 days. Stably Vem–resistant cells reached a confluence after two months. Generated Vem–resistant cells were named YUMM1.7_R and YUMMER_R to be distinguished from their isogenic parentals, YUMM1.7_P and YUMMER_P, respectively.
Yumm3
Manufactured by American Type Culture Collection
Sourced in United States
The YUMM3.3 is a versatile laboratory instrument designed for diverse scientific applications. It serves as a reliable platform for various experimental procedures, providing consistent and accurate results. The core function of the YUMM3.3 is to facilitate precise control and monitoring of experimental conditions, enabling researchers to conduct their investigations with precision and efficiency.
Automatically generated - may contain errors
Lab products found in correlation
Yumm1 g1, by American Type Culture Collection (2 mentions)
Yumm4, by American Type Culture Collection (2 mentions)
Yumm5, by American Type Culture Collection (2 mentions)
Pen strep, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Dmem f12 medium, by American Type Culture Collection (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Ultra low attachment 6 well plate, by Corning (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Puromycin, by Thermo Fisher Scientific (1 mentions)
Neomycin, by Thermo Fisher Scientific (1 mentions)
Versene solution, by Thermo Fisher Scientific (1 mentions)
Enzyme free dissociation buffer, by Thermo Fisher Scientific (1 mentions)
Yumm1, by American Type Culture Collection (1 mentions)
Murine b16 f10, by American Type Culture Collection (1 mentions)
3 protocols using yumm3
1
Establishing Vemurafenib-Resistant Melanoma Cell Lines
2
Culturing Murine Melanoma Cell Lines
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Murine B16-F10, YUMM1.7, YUMM1.G1, YUMM3.3, YUMM4.1, and YUMM5.2 melanoma cells were newly purchased from ATCC (Gaithersburg, MD), and YUMMER1.7D4 from Sigma (St. Louis, MO). All melanoma lines used were at low passage, < 70% confluency, and maintained in RPMI-1640 medium (Life Technologies, Carlsbad, CA) supplemented with 10% (v/v) fetal bovine serum (FBS, Sigma) and 1% (v/v) penicillin/streptomycin (Life Technologies) in standard culture flasks for 2D expansion or in 6-well ultra-low attachment plates (Corning, Glendale, AZ) for 3D tumor spheroid culture, as described5 (link). PD-1 (Pdcd1) OE and vector control B16-F10 melanoma cells were generated previously5 (link) and cultured in the presence of 1 μg/mL puromycin (Life Technologies) and 500 μg/mL neomycin (G418 sulfate, Life Technologies). PD-1−/− knockout (KO) B16-F10 melanoma cells were generated and validated as described below. Cells grown in 2D were harvested using 0.1% (v/v) versene solution (Life Technologies), as described5 (link), and 3D tumor spheroids were dissociated into single cell suspension after 5 days in culture for subsequent flow cytometric analysis using enzyme-free dissociation buffer (Thermo Fisher, Waltham, MA), per the manufacturer’s instructions.
3
Culturing Melanoma and YUMM3.3 Cells
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B16F10 melanoma cells were purchased from the MD Anderson Cancer Center Cell core (originally from the American Type Culture Collection (ATCC, VA, USA)). Cells were cultured in DMEM supplemented with 10% heat-inactivated fetal bovine serum (FBS). YUMM3.3 cells were purchased from ATCC. Cells were cultured in DMEM:F12 supplemented with 10% heat-inactivated FBS and 1% NEAA. Cell cultures were grown at 37°C in a humidified 5% CO2 atmosphere. All cell lines tested negative for mycoplasma.
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