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Human haemoglobin

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Human haemoglobin is a respiratory protein found in red blood cells that is responsible for transporting oxygen from the lungs to the body's tissues. It is a metalloprotein consisting of four globular protein subunits, each containing a heme group centered on an iron atom.

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Lab products found in correlation

Na2s2o4, by Merck Group (1 mentions) Sephadex g 25 pd 10 desalting column, by GE Healthcare (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions) Milli q system, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions)

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Haemoglobin

2 protocols using human haemoglobin

1

Purification and Characterization of Oxyhaemoglobin

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Human haemoglobin (Sigma Aldrich) was dissolved in water (20 mg/ml) and reduced by a 10-fold molar excess of sodium dithionite (Na2S2O4; Sigma Aldrich). Excess reductant was removed by gel filtration over Sephadex G-25 (PD10 desalting column; GE Healthcare) according to the manufacturer’s instructions. Oxyhaemoglobin (OxyHb) was eluted with 3.5 ml of water, and only the middle run was collected. The concentration of OxyHb was determined spectrophotometrically, as described in.26 Aliquots of the OxyHb stock solution were kept at −80°C, thawed on the day of experimentation and discarded after use.
Borgognone A., Shantsila E., Worrall S.M., Prompunt E., Loka T., Loudon B.L., Chimen M., Ed Rainger G., Lord J.M., Turner A., Nightingale P., Feelisch M., Kirchhof P., Lip G.Y., Watson S.P., Frenneaux M.P, & Madhani M. (2018). Nitrite circumvents platelet resistance to nitric oxide in patients with heart failure preserved ejection fraction and chronic atrial fibrillation. Cardiovascular Research, 114(10), 1313-1323.
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2

Synthesis and Characterization of 13C-PQ and PQ-5,6-Orthoquinone

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13C-labelled PQ (13C-PQ, with 6 isotopic carbons in the quinoline nucleus), and PQ-5,6-orthoquinone were synthesized as previously reported [23 (link), 32 (link)]. HPLC-grade acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Water for the HPLC mobile phase was purified in a Milli-Q system (Millipore, Bedford, MA, USA). Human haemoglobin (lyophilized powder), formic acid and glucose were purchased from Sigma-Aldrich (St Louis, MO, USA).
Fasinu P.S., Nanayakkara N.P., Wang Y.H., Chaurasiya N.D., Herath H.M., McChesney J.D., Avula B., Khan I., Tekwani B.L, & Walker L.A. (2019). Formation primaquine-5,6-orthoquinone, the putative active and toxic metabolite of primaquine via direct oxidation in human erythrocytes. Malaria Journal, 18, 30.
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