Gs 5806

Manufactured by MedChemExpress

Sourced in United States

GS-5806 is a chemical compound used as a laboratory tool for research purposes. It is a small-molecule inhibitor that can be utilized in various experimental studies. The core function of GS-5806 is to serve as a research tool, without further interpretation of its intended use.

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Lab products found in correlation

Bms433771, by Merck Group (2 mentions) Tmc353121, by MedChemExpress (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions) Ribavirin, by Merck Group (1 mentions) Heparin, by Merck Group (1 mentions) Ak 0529, by MedChemExpress (1 mentions) Hep 2, by American Type Culture Collection (1 mentions) D luciferin potassium salt, by Merck Group (1 mentions) Ribavirin, by Topscience (1 mentions) Hep 2 cells, by American Type Culture Collection (1 mentions)

4 protocols using gs 5806

Compound Preparation for Cell Assay

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CPM (S1146) was purchased from Selleck Chemicals. RSV604 (SML1695) and BMS433771 (BMS0022) were purchased from Sigma–Aldrich. ALS8176 (HY–12983A), GS5806 (HY–16727), and Ziresovir (HY–109142) were purchased from MedChem Express. Compounds were solubilized at 8 mM in 100% v/v DMSO, then serially diluted to the desired concentrations in DMEM with 2% fetal bovine serum (FBS) and 0.25% v/v DMSO in the assay.

Gao Y., Cao J., Xing P., Altmeyer R, & Zhang Y. (2021). Evaluation of Small Molecule Combinations against Respiratory Syncytial Virus In Vitro. Molecules, 26(9), 2607.

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Screening Antiviral Compounds against RSV

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HEp-2 and HEK293T cells were purchased from the American Type Culture Collection (ATCC) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 2 mM l-glutamine. RSV A2 was obtained from Wuhan University, China. RSV A2-F_K394R was isolated and identified in our previous study (29 (link)). All the viruses were grown in HEp-2 cells and stored at −70°C. Viral titers were determined by plaque assay. Salvianolic acid R (LF-6) was isolated from the aerial part of Mesona chinensis Benth. (Lamiaceae) by the authors, following our previously reported procedure (30 (link)). The purity of LF-6 (>98%) was validated through high-performance liquid chromatography (HPLC) analysis. Ribavirin, heparin, and BMS-433771 were purchased from Sigma-Aldrich, Inc. TMC-353121, JNJ-53718678, GS-5806, and AK-0529 were purchased from MedChem Express, Inc.

Tang W., Li Y., Song Q., Wang Z., Li M., Zhang Q., Wang Y., Ye W, & Li Y. (2021). Mechanism of Cross-Resistance to Fusion Inhibitors Conferred by the K394R Mutation in Respiratory Syncytial Virus Fusion Protein. Journal of Virology, 95(20), e01205-21.

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Antiviral Compound Evaluation

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P13 and Ribavirin were purchased from Topscience Company (Shanghai, China). GS-5806 (fusion inhibitor) and RSV-604 (N protein inhibitor) were purchased from MCE (MedChemExpress, Monmouth Junction, NJ, USA). D-luciferin potassium salt was purchased from Sigma (Sigma, St. Louis, MO, USA). Ribavirin was dissolved in deionised water, and the synthetic compounds (4–49 C and 1-HB-63) were dissolved in DMSO to prepare the working solution.

Cheng N., Jiang N., Fu Y., Xu Z., Peng X., Yu J., Cen S., Wang Y., Zhang G., Zheng Y, & He J. (2022). The mechanism and pharmacodynamics of 2-((1H-indol-3-yl)thio/sulfinyl)-N-pheny acetamide derivative as a novel inhibitor against human respiratory syncytial virus. Journal of Enzyme Inhibition and Medicinal Chemistry, 37(1), 2598-2604.

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Respiratory Syncytial Virus (RSV) Cell Culture Protocol

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DMEM (catalogue no. 41965-039), PBS (catalogue no. 14190-094) and non-essential amino acid solution (NEAA; catalogue no. 11140-035) were obtained from Thermo Fisher Scientific. FBS was obtained from Hyclone (catalogue no. SV30160.03) and heat inactivated at 56°C for 30 min. HEp-2 cells and RSV-A Long strain were obtained from ATCC (catalogue no. CCL-23 and VR-26, respectively). HuAECs of bronchial origin in an air–liquid interface cell culture system and MucilAir medium were obtained from Epithelix (catalogue no. EP01MD and EP04MM, respectively), a Swiss company that has developed a technology that allows epithelium to reach a homeostatic state with a slow turnover. It is a ready-to-use product and commercially available (http://www.epithelix.com/). ALS-8112 and ALS-8176,¹¹ (link) AZ-27¹⁴ (link) and PC786¹⁵ were synthesized according to published procedures (purity >98%). GS-5806 and TMC353121 were obtained from MedChemExpress (NJ, USA) and ribavirin was from ICN Pharmaceuticals (CA, USA).

Mirabelli C., Jaspers M., Boon M., Jorissen M., Koukni M., Bardiot D., Chaltin P., Marchand A., Neyts J, & Jochmans D. (2018). Differential antiviral activities of respiratory syncytial virus (RSV) inhibitors in human airway epithelium. Journal of Antimicrobial Chemotherapy, 73(7), 1823-1829.

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