Human aortic SMCs (HASMC) were purchased from Lonza. Cells were cultured using Smooth Muscle Cell Growth Medium (SmGM) containing 5% fetal bovine serum with growth factors and antibiotics (Lonza, Cat no. cc-4149) in humidified incubator containing 5% CO2 at 37 °C. All experiments were performed with HASMCs at passages 5–7.
Cc 4149
Manufactured by Lonza
Sourced in Switzerland
The Cc-4149 is a laboratory equipment designed for general scientific applications. It serves as a versatile tool for various experiments and analytical procedures. The core function of the Cc-4149 is to provide a controlled and consistent environment for sample preparation, analysis, or other laboratory processes. The specific details and intended use of this product are not included in this factual and unbiased description.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions)
Control sirna, by Merck Group (2 mentions)
Smgm 2 medium, by Lonza (2 mentions)
Adamts9 sirna sasi hs02 00371041, by Merck Group (2 mentions)
Vcan sirna silencer select s229335, by Thermo Fisher Scientific (2 mentions)
Hasmc, by Lonza (1 mentions)
Cc 2581, by Lonza (1 mentions)
4 protocols using cc 4149
1
Culturing Human Aortic SMCs
2
Recombinant Expression of ETA Receptor Variants
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Jump-In T-Rex HEK-293 cells (HEK-TRex) recombinantly expressing FLAG-tagged ETA receptor variants were cultivated in DMEM, GlutaMAX-I, containing 4.5 g/l D-glucose, pyruvate, 1% non-essential amino acids, 10% dialyzed FCS, 1000 µg/ml geneticin, 100 U/ml penicillin, and 5 µg/ml blasticidin. PASMC were purchased from Lonza (CC-2581) and cultivated in Lonza Clonetics SmBM growth medium (CC-3181) with SmGM-2 SingleQuot supplements and 5% FCS (CC-4149). PASMC of passage 3 to 5 were used for all experiments.
3
Regulation of ADAMTS9 and VCAN by siRNA
4
Regulation of ADAMTS9 and VCAN by siRNA
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HUSMCs (cc-2562; Lonza, Basel, Switzerland) were maintained on tissue culture plastic in HUSMC complete media (SmGM-2 medium [cc-3181; Lonza] supplemented with 5% fetal bovine serum, growth factors [cc-4149; Lonza]), 100 U/mL penicillin, and 100 mg/mL streptomycin. Cells in passages 3–8 were used for all experiments. HUSMCs in six-well plates were transfected with ADAMTS9 siRNA (SASI_Hs02_00371041; Sigma-Aldrich), VCAN siRNA (Silencer select s229335; Ambion, Waltham, MA), or control siRNA (SIC001; Sigma-Aldrich) using Lipofectamine RNAiMAX (13778; Invitrogen, Carlsbad, CA) upon achieving 60% confluence and harvested 48 hr after transfection. For rescue experiments, ADAMTS4 or ADAMTS5 at 0.1 U/mL (Vankemmelbeke et al., 2003 (link)) was added at the time of transfection and at 24 hr or cells were plated on fibronectin (Supplemental Experimental Procedures ).
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