Synergy 2 modular multimode reader

Manufactured by Agilent Technologies

Sourced in United States

The Synergy 2 modular multimode reader is a versatile instrument designed for various applications in life science research. It provides a platform for performing absorbance, fluorescence, and luminescence measurements to support a wide range of assays and experiments.

Automatically generated - may contain errors

Lab products found in correlation

Hyclone, by GE Healthcare (1 mentions) Mant gdp, by Thermo Fisher Scientific (1 mentions) Eclipse e800 epifluorescence microscope, by Nikon (1 mentions)

5 protocols using synergy 2 modular multimode reader

Cytotoxicity Assessment of Anti-cancer Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mycoplasma negative erythroleukemia cell lines CB3, CB7, K562 and HEL were all previously generated in our group or obtained from other laboratories, as described [10 (link)]. These cell lines were maintained in Dulbecco’s Modified Eagle Medium mixed with 5% fetal bovine serum (HyClone, GE Healthcare, Australia).
For IC50 determinations, cells (8 × 10³) were seeded in 96-well plates and treated with A1541, A1542, A1543 and Cedrelone at different concentrations for 3 days. Cells were then used in an MTT assay by adding 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to the culture for 4 h. Following removal of the supernatant, 200 ml DMSO was added to dissolve the formazan crystals. The absorbance was read using a Synergy2 modular Multi-Mode Reader (BioTek Instruments, Inc., Winooski, VT, USA) at 490 nm.

Wang N., Fan Y., Yuan C.M., Song J., Yao Y., Liu W., Gajendran B., Zacksenhaus E., Li Y., Liu J., Hao X.J, & Ben-David Y. (2019). Selective ERK1/2 agonists isolated from Melia azedarach with potent anti-leukemic activity. BMC Cancer, 19, 764.

+ Open protocol

+ Expand

Quantitative GEF Assay for Rab GTPases

Check if the same lab product or an alternative is used in the 5 most similar protocols

The GEF assay was performed as described previously (Sakaguchi et al., 2015 (link)), with some modifications. RAB-10 tagged with C-terminal His6 was purified as described previously (Ebine et al., 2014 (link)). 50 µM MANT-GDP (Invitrogen) was incubated with 500 pmol RAB-10-His6 in the preloading buffer at 25°C for 60 min, and 10 mM MgCl₂ was added to stop the reaction. 200 pmol preloaded Rab-His6 was incubated with 100 pmol of the indicated GST-tagged proteins in GEF buffer (50 mM Tris, pH 8.0, 150 mM NaCl, and 0.5 mM MgCl₂) at 25°C for 100 s The nucleotide exchange reaction was started by adding the hydrolysis-resistant GTP analogue GMP-PNP (guanylyl-imidodiphosphate; Calbiochem) at a final concentration of 0.1 mM. The nucleotide exchange reaction was recorded using a Synergy 2 modular multimode reader (BioTek) at an excitation wavelength of 366 nm and an emission wavelength of 443 nm.

Liu H., Wang S., Hang W., Gao J., Zhang W., Cheng Z., Yang C., He J., Zhou J., Chen J, & Shi A. (2018). LET-413/Erbin acts as a RAB-5 effector to promote RAB-10 activation during endocytic recycling. The Journal of Cell Biology, 217(1), 299-314.

+ Open protocol

+ Expand

Cytotoxicity Evaluation of MQ-16 and Imatinib

Check if the same lab product or an alternative is used in the 5 most similar protocols

K562 cells and HL-7702 cells (8 × 10³ cells/well) were exposed to MQ-16 (25–400 nM) or Imatinib (0.1875–3 μM) for 72 h. Control cells were treated with an equal volume of dimethyl sulfoxide (DMSO) (final concentration, <0.1%). Ten microliters of 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were added to cells cultured in 96-well plates for 4 h, and the absorbance of formazan products was measured at 490 nm using the Synergy 2 modular Multi-Mode Reader (BioTek, Winooski, VT, USA). Then, we used the FORECAST function to calculate the IC₅₀ at 72 h. K562 cells were seeded in 96-well plates with MQ-16 (50, 100, 200, or 400 nM) for different times, and the cell inhibition rate and the growth curve were then analyzed using GraphPad Prism 8. The inhibition rate was calculated as follows: inhibition rate = (control group OD₄₉₀ - treatment group OD₄₉₀)/control group OD₄₉₀ × 100%.23 (link)

Yang X., Wu X., Wu X., Huang L., Song J., Yuan C., He Z, & Li Y. (2022). The Flavagline Compound 1-(2-(dimethylamino)acetyl)-Rocaglaol Induces Apoptosis in K562 Cells by Regulating the PI3K/Akt/mTOR, JAK2/STAT3, and MAPK Pathways. Drug Design, Development and Therapy, 16, 2545-2557.

+ Open protocol

+ Expand

Assessing Intracellular ROS via Imaging and Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Intracellular ROS accumulation was measured by immunocytochemistry as well as flow cytometry by using CM-H2DCFDA. The cells were stimulated with HG (25mM) without or with didymin (20 μM) for 3h. The cells were stained with CM-H2DCFDA for 15 min and photographs (20×) were taken under the microscope (Nikon Eclipse E800 epifluorescence microscope) and fluorescence intensity was determined at 495/517 (excitation/emission) by using a florescence microplate reader (Biotek Synergy 2 modular multimode reader). In another set of experiments, cells were stained with CM-H2DCFDA for 15 min and analyzed immediately by a Flow Cytometer (BD LSRII Fortessa). Data analysis was performed using FlowJo (Treestar, Ashland, OR, USA). Further, HUVECs treated with HG without or with didymin were incubated with hydroxyphenyl fluorescein (HPF), which stain hydroxyl radical and peroxinitrite radicals, and fluorescence intensity was determined by flow cytometry.

Shukla K., Sonowal H., Saxena A, & Ramana K.V. (2018). Didymin prevents hyperglycemia-induced human umbilical endothelial cells dysfunction and death. Biochemical pharmacology, 152, 1-10.

+ Open protocol

+ Expand

Actin Polymerization and Depolymerization Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Actin Polymerization Biochem Kit: Muscle Actin (BK003; Cytoskeleton) was used for both polymerization and depolymerization assays. The fluorescence intensity of the pyrene-conjugated actin was measured to monitor actin polymerization or depolymerization dynamics. In actin polymerization assay, 10 µM pyrene-G-actin was prepared by the standard protocol. The purified GST-tagged proteins were added into pyrene-G-actin preparation. The fluorescence intensity was recorded every minute over 20 min using a Synergy 2 modular multimode reader (BioTek Instruments) at an excitation wavelength of 350 nm and an emission wavelength of 407 nm. The purpose of this step is to detect whether the protein itself has a polymerization activity. Then, actin polymerization buffer was added to the reaction, and the fluorescence intensity was recorded for an additional 100 min. For actin depolymerization assay, 5 µM pyrene-G-actin was used for the preparation of F-actin. F-actin was diluted to 1 µM, and the fluorescence intensity was recorded every minute over 3 min to obtain the baseline. The purified GST-tagged proteins were then added into pyrene-F-actin preparation, and the fluorescence signal was recorded for an additional 60 min.

Yan Y., Liu S., Hu C., Xie C., Zhao L., Wang S., Zhang W., Cheng Z., Gao J., Fu X., Yang Z., Wang X., Zhang J., Lin L, & Shi A. (2021). RTKN-1/Rhotekin shields endosome-associated F-actin from disassembly to ensure endocytic recycling. The Journal of Cell Biology, 220(5), e202007149.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!