Anti mmp2
Anti-MMP2 is a laboratory reagent that can be used to detect and quantify the presence of the Matrix Metalloproteinase 2 (MMP2) protein. MMP2 is an enzyme involved in the breakdown of the extracellular matrix. This reagent can be utilized in various research applications that require the measurement or analysis of MMP2 levels.
Lab products found in correlation
123 protocols using anti mmp2
Western Blot Analysis of MMPs and Collagens
Western Blot Analysis of Protein Markers
Cells were washed three times with PBS and then lysed in pre-cooled radioimmunoprecipitation (RIPA) assay buffer on ice for 10 min. After removal of cell debris by centrifugation (12,000g, 10 min), protein concentration of supernatants was measured by BCA protein assay kit (Thermo Fisher Scientific). After boiling for 5 min in sample buffer, equal amount of proteins of different groups were separated by SDS–PAGE, and transferred onto to a nitrocellulose membrane (Millipore, Bredford, USA). After blocking with 5% skim milk, the membranes were incubated with the primary antibodies at 4°C overnight with agitation, followed by incubation with corresponding secondary antibodies for 1 h at room temperature with agitation. Reactive protein was then detected using ECL chemiluminescence system (Bio-Rad, Richmond, CA, USA).
Cab45-G Knockdown Impacts EMT Signaling
Quantification of Signaling Pathways
Western Blotting for EMT and Apoptosis
Western Blot Analysis of Proteases
Histological Analysis of Atherosclerotic Plaques
Western Blot Analysis of Apoptosis and Cell Cycle Markers
The primary antibodies used in this study are purchased from Abcam (Cambridge, UK) as followed: anti-Bax (1:1000), anti-Bcl- 2 (1:1000), anti-caspase 3 (1:1000,), anti-caspase 9 (1:1000), anti- Cyclin D1 (1:1000), anti-PCNA (1:1000), anti-Cox-2 (1:1000), anti-MMP2 (1:1000), anti-MMP9 (1:1000), anti-GTPBP4 (1:1000), and anti-β-actin (1:1000, internal control). All reacts with human proteins.
Protein Expression Analysis by Western Blot
Cellular Signaling Pathway Profiling
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