The 50 nm polystyrene nanoparticle (PS-NPs) beads (5% w/v) were purchased commercially from Janus New-Materials (Nanjing, China) (refer to the Supplementary Materials for more detailed parameters). SHSY-5Y cells, the clonal subline of neuroblastoma SK-N-SH cell line, were obtained from American Type Culture Collection (ATCC, Rockville, MD, USA). The 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di- phenytetrazo-liumromide (MTT), Rhodamine 123 (Rh 123), 2,7-dichlorodi- hydrofluorescein diacetate (DCFH-DA), tert-Butyl hydroperoxide (tBHP), and N-Acetylcysteine (NAC) were obtained from Sigma (St. Louis, MO, USA). The annexin V-fluoresceine isothiocyanate/propidium iodide (annexin V-FITC/PI) apoptosis detection kit was obtained from Sungene (Tianjin, China). Protein extraction and assay kit was purchased from Thermo (MA, USA). Fluo 3-AM was acquired from Dojindo (Kyushu, Japan). Lactate dehydrogenase (LDH) cytotoxicity assay kit and adenosine 5′-triphosphate (ATP) detection kit were obtained from Beyotime (Shanghai, China).
Lactate dehydrogenase ldh cytotoxicity assay kit
Manufactured by Beyotime
Sourced in China
The Lactate dehydrogenase (LDH) cytotoxicity assay kit is a colorimetric method for the quantitative determination of lactate dehydrogenase, an enzyme released upon cell death or damage. The assay measures the activity of LDH in the culture medium, which is proportional to the number of lysed or damaged cells.
Automatically generated - may contain errors
Lab products found in correlation
Cell counting kit 8 cck 8, by Dojindo Laboratories (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Cell counting kit 8 cck 8, by Beyotime (3 mentions)
Caspase 3 activity assay kit, by Beyotime (2 mentions)
Lipopolysaccharide lps, by Merck Group (2 mentions)
Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Ribofect cp transfection kit, by RiboBio (2 mentions)
Il 1β elisa kit, by R&D Systems (2 mentions)
Trypsin, by Thermo Fisher Scientific (2 mentions)
Lipid peroxidation malondialdehyde mda assay kit, by Beyotime (2 mentions)
Sh sy5y cell, by American Type Culture Collection (1 mentions)
Sk n sh cell line, by American Type Culture Collection (1 mentions)
N acetylcysteine, by Merck Group (1 mentions)
Tert butyl hydroperoxide tbhp, by Merck Group (1 mentions)
Fluo 3 am, by Dojindo Laboratories (1 mentions)
Tdt mediated dutp nick end labeling tunel apoptosis assay kit, by Beyotime (1 mentions)
Alexa fluor 488 dextran, by Thermo Fisher Scientific (1 mentions)
Jc 1 mitochondrial membrane potential assay kit, by Beyotime (1 mentions)
Hoechst staining kit, by Beyotime (1 mentions)
28 protocols using lactate dehydrogenase ldh cytotoxicity assay kit
1
Polystyrene Nanoparticle Toxicity in Neuroblastoma Cells
2
Multifunctional Nanoparticles for Targeted Drug Delivery
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Cetyltrimethylammonium chloride (CTAC), folic acid (FA), 3-aminopropyltriethoxysilane (APTES), tetraethyl orthosilicate (TEOS), dopamine hydrochloride, triethanolamine (TEA) and N,N-dimethylformamide (DMF) were purchased from Aladdin Reagent (Shanghai, China). Human serum albumin (HSA) was supplied by Biosynthesis Biotechnology Co. Ltd (Beijing, China). The cell counting kit-8 (CCK-8) was acquired from Dojindo Laboratories (Kumamoto, Japan). Caspase-3 activity assay kit, lactate dehydrogenase (LDH) cytotoxicity assay kit, adenosine triphosphate (ATP) assay kit, 4′,6-diamidino-2-phenylindole (DAPI), Hoechst staining kit, TdT-mediated dUTP Nick-End Labeling (TUNEL) apoptosis assay kit and mitochondrial membrane potential assay kit (JC-1) were purchased from Beyotime Institute of Biotechnology (Shanghai, China). LysoTracker Green was purchased from Yeasen Biotech Co. Ltd (Shanghai, China). MitoLite Blue was purchased from AAT Bioquest Inc. (CA, USA). Alexa Fluor 488-dextran (10 000 MW) was purchased from Thermo Fisher Scientific Inc. (MA, USA). All other reagents were of analytical grade. Ultrapure water (18.2 MΩ) was prepared with a Milli-Q water purification system (Merck Millipore, USA) and used in all experiments.
3
Cysltr1 Expression in APAP-Induced Injury
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APAP and dimethylsulfoxide (DMSO) was purchased from Sigma-Aldrich (St. Louis, USA). Montelukast and zafirlukast were purchased from meilunbio (Dalian, China). GSH/glutathionedisulfide(GSSG)assaykit was obtained from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The lactate dehydrogenase (LDH) cytotoxicity assay kit and the mitochondrial membrane potential assay kit with JC-1 were offered by the Beyotime Institute of Biotechnology (Shanghai, China). Primary antibodies according Cysltr1 and β-actin were purchased from Abclonal (Wuhan, China), and the secondary antibody was purchased from Jackson ImmunoResearch (PA, USA).
4
In Vitro Cytotoxicity of Terpene Alcohols on HUVECs
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TA was purchased from Wuhan Lullaby Pharmaceutical Chemical Co., Ltd. (Wuhan, China). The chemicals 1-hexanol, 1-octanol, and 1-nonanol were bought from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Dulbecco’s Modified Eagle’s Medium (DMEM) and cell-culture Petri dishes were obtained from Corning (New York, NY, USA). Penicillin–streptomycin was obtained from Gibco (Waltham, MA, USA). Trypsin was obtained from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) was obtained from Zhejiang Tianhang Biotechnology Co., Ltd. (Hangzhou, China). A Cell Counting Kit-8 (CCK-8) and a Lactate Dehydrogenase (LDH) Cytotoxicity Assay Kit were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China). All of the other reagents were of analytical grade and used without further purification. Human umbilical vein endothelial cells (HUVECs) were provided by ATCC (Rockefeller, MD, USA). Fluorescent Cy7 was obtainedd from Beijing Solarbio Life Sciences Co., Ltd. (Beijing, China). Kunming (KM) mice and New Zealand rabbits were provided by the Animal Center of Shenyang Pharmaceutical University. All animal experiments and procedures were reviewed and approved by the Animal Care and Use Committee at the Shenyang Pharmaceutical University (Ethical Committee approval number: SYPU-IACUC-C2019-12-14-105; date of approval: 14 December 2019).
5
Autophagy Modulation by Epirubicin and BAF1
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Epirubicin was from Pfizer Pharmaceuticals (Dalian, China). Bafilomycin A1 (BAF1) was from Sigma‐Aldrich (Shanghai) Trading (Shanghai, China). The annexin V‐PE/7‐AAD Apoptosis Assay Kit was from Nanjing KeyGen Biotech (Nanjing, China). The caspase‐9 activity assay kit, the lactate dehydrogenase (LDH) cytotoxicity assay kit, and the Cell Counting Kit‐8 (CCK‐8) were from the Beyotime Institute of Biotechnology (Shanghai, China). Anti‐MAPLC3β (H50) and anti‐Ambra1 antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti‐GAPDH antibody was from MultiSciences (Lianke) Biotech (Shanghai, China). Anti‐p62, ATG12, and ATG5 were from Abcam (HongKong, China). Anti‐Beclin 1 was from CST (Danvers, MA, USA).
6
Dendritic Cell Differentiation and Activation
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Roswell Park Memorial Institute (RPMI) 1640 culture medium and fetal bovine serum (FBS) were purchased from HyClone (Logan, UT, USA). Recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF) and recombinant human interleukin-4 (rhIL-4) were purchased from PeproTech (Rocky Hill, CT, USA). Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich Co (St Louis, MO, USA). Fluorescein isothiocyanate (FITC)-conjugated anti-CD80, anti-CD83, and phycoerythrin (PE)-conjugated anti-CD86, anti-HLA-DR were purchased from BioLegend (San Diego, CA, USA). Anti-B7-H1 polyclonal antibody was purchased from Santa Cruz Biotech (Dallas, TX, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Tokyo, Japan). IL-10 enzyme-linked immunosorbent assay (ELISA) kit, IL-12 ELISA kit, and IFN-γ ELISA kit were purchased from R&D Systems (Minneapolis, MN, USA). Lactate dehydrogenase (LDH) cytotoxicity assay kit was purchased from Beyotime (Nanjing, People’s Republic of China).
7
RNase A Cytotoxicity Assays
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Bovine pancreatic RNase A, zinc acetate, 2-methylimidazole, and fluorescein isothiocyanate (FITC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Enzymatic activity was detected by RNase Alert® kit, which was obtained from Integrated DNA Technologies, Inc. (Coralville, IA, USA). BCA protein assay kit was obtained from BioTeke (Beijing, China). The LIVE/DEAD® Viability/Cytotoxicity kit was provided by ThermoFisher (Grand Island, NE, USA). One-step TUNEL cell apoptosis detection kit (green fluorescence) and lactate dehydrogenase (LDH) cytotoxicity assay kit were purchased from Beyotime (Jiangsu, China). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Kangyuan Co. (Beijing, China) and Gibco (Grand Island, NE, USA), respectively. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Amersco (Solon, OH, USA). All other reagents were of the highest grade available and used as received.
8
NK Cell-Mediated Cytotoxicity Assay
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The transfected cells were cocultured with NK cells at different effector-to-target (E:T) cell ratios as indicated for 4 h. The cytotoxicity of NK cells was assessed using a Lactate Dehydrogenase (LDH) Cytotoxicity Assay Kit (Beyotime, Shanghai, China) following the manufacturer’s instructions.
9
Biosynthesis and Cytotoxicity of Gold Nanoparticles
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Bovine serum albumin was purchased from Sigma. Au(iii ) chloride trihydrate, sodium citrate, NaCl, NaH2PO4·3H2O, and Na2HPO4·12H2O were all analytical grade and purchased from Chinese Sinopharm Chemical Reagent Co., Ltd. Double distilled water was used. 3-(4,5-Dimethyl-2-thiazolyl)-2-5-diphenyltetrazolium bromide (MTT) assay, Roswell Park Memorial Institute (RPMI) 1640 with 1% l -glutamine, phosphate buffered saline (0.01 M, pH 7.2) and fetal bovine serum were purchased form Gibco (Life Technologies, Thermo Fisher Scientific, Waltham, MA). Lactate dehydrogenase (LDH) cytotoxicity assay kit were purchased form Beyotime Institute of Biotechnology (Shanghai, China). FITC were purchased from Lumiprobe-Life Science.
10
LDH Cytotoxicity Assay for Cell Death
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Cell death was evaluated using the Lactate Dehydrogenase (LDH) Cytotoxicity Assay Kit (Beyotime, Shanghai, China). The HIEC-6 cells were grown in 96-well plates for 24 h and subsequently subjected to various treatments. The level of LDH in the cell culture supernatant was measured according to the manufacturer’s instruction.
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