MKN28, AGS, MGC-803, SGC-7901, BGC-823, and MKN45 human gastric cancer cell lines and a normal gastric cell line GES-1 were obtained from Life Technologies (Gaithersburg, MD, USA) and were maintained in a humidified incubator at 37°C and 5% CO2. MGC-803, MKN45, and AGS cells were cultivated in RPMI-1640 medium (Life Technologies), and BGC-823, MKN28, and SGC-7901 cells were cultivated in DMEM medium (Life Technologies) plus 10 mM glucose, containing 10% fetal bovine serum, 100 μg/ml streptomycin, and 100 U/ml penicillin (all from Gibco, Grand Island, NY, USA).
Ges 1
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
The GES-1 is a laboratory instrument designed for gas exchange measurements. It provides accurate and reliable data on the exchange of gases, such as oxygen and carbon dioxide, between a sample and its surrounding environment.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (58 mentions)
Sgc 7901, by Thermo Fisher Scientific (49 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (46 mentions)
Mgc 803, by Thermo Fisher Scientific (37 mentions)
Hgc 27, by Thermo Fisher Scientific (34 mentions)
Mkn45, by Thermo Fisher Scientific (34 mentions)
Bgc 823, by Thermo Fisher Scientific (33 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (27 mentions)
Rpmi 1640, by Thermo Fisher Scientific (20 mentions)
Mkn28, by Thermo Fisher Scientific (17 mentions)
Penicillin, by Thermo Fisher Scientific (17 mentions)
Streptomycin, by Thermo Fisher Scientific (16 mentions)
Ges 1, by National Collection of Authenticated Cell Cultures (12 mentions)
Hgc 27, by National Collection of Authenticated Cell Cultures (11 mentions)
Nci n87, by Thermo Fisher Scientific (11 mentions)
Bgc823, by National Collection of Authenticated Cell Cultures (10 mentions)
Mgc 803, by National Collection of Authenticated Cell Cultures (10 mentions)
Sgc 7901, by National Collection of Authenticated Cell Cultures (10 mentions)
Mkn45, by American Type Culture Collection (9 mentions)
Dmem medium, by Thermo Fisher Scientific (8 mentions)
100 protocols using ges 1
1
Cultivation of Gastric Cancer Cell Lines
2
Culturing Gastric Cell Lines
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Normal gastric mucosal cells GES-1 (CC-Y1572) and gastric carcinoma cells SGC-7901 (CC-Y1456), BGC-823 (CC-Y1071), MKN-45 (CC-Y1358), and HGC27 (CC-Y1228) were purchased from EK-Bioscience (China). All cells were identified by STR. GES-1 and HGC27 needed to be cultured in DMEM (11965118, Life Technologies, USA) containing 10% fetal bovine serum (FBS, C0251, Beyotime, China), while SGC-7901, MKN-45, and BGC-823 cells were cultured in RPMI-1640 medium (SH30809.01, HyClone, USA) containing 10% fetal bovine serum. The above cells were cultured in a Thermo Scientific CO2 cell culture incubator containing 5% CO2 at 37°C.
3
Culturing Human Cell Lines
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Human gastric epithelial cell line, GES-1, was obtained from Cancer Institute and Hospital, Chinese Academy of Medical Sciences (Beijing, China). Human gastric cancer cell lines, AGS, MGC-803 and SGC-7901, were obtained from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China). Human embryonic kidney cell line, HEK 293T, was obtained from GeneChem Co., Ltd. (Shanghai, China). GES-1, AGS, MGC-803 and SGC-7901 were grown in RPMI Medium 1640 (Life Technologies) plus 10% fetal bovine serum (FBS). HEK 293T was grown in DMEM (Life Technologies) plus 10% FBS. All cell lines were grown at 37°C in a humidified atmosphere with 5% CO2. Cells were counted using a TC10 Automated Cell Counter (Bio-Rad).
4
Gastric Epithelial Cell Culture Protocols
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Human normal gastric mucosa epithelial cell line GES-1 was obtained from Gefan Biological Technology (Shanghai, China). Human gastric cancer cell lines HGC-27, AGS, SGC-7901 were purchased from the Institutes for Biological Sciences at the Chinese Academy of Sciences (Shanghai, China). Human gastric cancer cell line MGC-803 was obtained from the Cell Resource Center, Institute of Basic Medicine, Chinese Academy of Medical Sciences (Beijing, China). GES-1, HGC-27 and SGC-7901 cells were cultured in RPMI 1640 medium (Life Technologies, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS; Life Technologies) at 37 °C in humidified air with 5% CO2. The other cell lines were cultured in high-glucose DMEM (Life Technologies) supplemented with 10% FBS. Cells have been regularly tested for Mycoplasma and are free of contamination.
5
Cell Culture Protocols for Gastric and Stem Cells
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The gastric cell lines GES-1, MGC-803, MKN-45, SGC-7901, HGC-27, AGS and embryonic kidney cell line HEK-293T were obtained from the Cell Storage Center of Wuhan University (Wuhan, China). Human umbilical cord-derived adipose-derived mesenchymal stem cells (hUC-MSCs) were derived from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in the following media: GES-1 and HEK-293T in DMEM supplemented with 10% FBS (Gibco, USA). MGC-803, MKN-45, SGC-7901 and HGC-27 in RPMI-1640 with 10% FBS. AGS cells were cultured in DMEM/F12 with 10% FBS, hUC-MSCs were cultured in Human umbilical cord blood mesenchymal stem cell complete medium (Pricella, China), supplemented with 1% penicillin/streptomycin (Meilunbio, China). Cell lines were tested and verified to be mycoplasma negative using MycoAlert™ PLUS (Lonza, Switzerland). All cells were incubated at 37°C in a humidified atmosphere of 5% CO2.
6
Gastric Cancer Cell Line Cultivation
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The GC cell lines SGC7901, BGC803, AGS, MKN45, BGC823, MGC803, HGC27 and the gastric mucosal epithelial cell lines GES-1 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). SGC7901, AGS, BGC823, MKN45, BGC803, MGC803, HGC27, and GES-1 cells were cultured in RPMI-1640 medium (Gibco, Grand Island, NY, USA). All media were supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin (Sigma-Aldrich, St Louis, MO, USA), and 100 mg/mL streptomycin (Sigma-Aldrich). Cycloheximide (CHX), proteasome inhibitor MG132, chloroquine diphosphate (CQ), and GSK-3β inhibitor CHIR-99021 were bought from Selleck Chemicals (Houston, TX, USA). Alkaline phosphatase (CIP) was obtained from Roche (Roche Applied Science, Indianapolis, IN, USA). The GSK-3β inhibitor LiCl was obtained from Sigma Aldrich.
7
Culturing Human Gastric Cell Lines
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Human gastric cell lines SGC-7901, BGC-823, MGC-803, AGS, and GES1 were obtained from the Committee of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and cultured in RPMI-1640 (Gibco, USA) medium supplemented with 10% fetal bovine serum (BI, USA) in a humidified incubator (Thermal, USA) at 37.0°C with 5% CO2.
8
Cultivation of Gastric Cell Lines
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Human normal gastric epithelial cells (Ges-1) were obtained from Guangdong Hybribio Biotech Co., Ltd (Guangdong, China). And GC cell lines AGS, MNK-45, HGC-27, SGC-7901 were obtained from Hunan Fenghui Biotechnology Co., Ltd (Hunan, China). Ges-1, HGC-27, MNK-45 were cultured in RPMI-1640 medium (Gibco, USA) plus 10% fetal bovine serum (Procell, Wuhan, Hubei, China) and 1% penicillin and streptomycin (ABT920; G-clone, Beijing, China). The basal medium for AGS and SGC-7901 were Ham’s F12 (Procell, Las Vegas, NV, USA) and Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Waltham, MA, USA), respectively, and the rest of the culture conditions were the same. All were incubated at 37 °C in a humidified incubator with 5% CO2.
9
Gastric Cancer Cell Lines and Animal Model
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A total of 6 human gastric cancer cell lines including AGS, MGC-803, SGC-7901, BGC-823, MKN-45, and MKN-28 were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The human gastric epithelial cell line GES-1 was purchased from the Beijing Institute of Cancer Research (Beijing, China). Notably, the use of human gastric epithelial cell line GES-1 was approved by the Ethics Committee of Hangzhou Medical College. SGC-7901, BGC-823, MKN-45, MKN-28, and GES-1 cell lines were cultured in RPMI-1640 medium (Gibco), AGS cell line was cultured in DMEM culture medium (Gibco), and MGC-803 cell line was cultured in DF12 culture medium (Gibco). The concentration of fetal bovine serum (Gibco) in all culture media was 10%.
BALB/c nude mice aged 5 weeks were purchased from Shanghai Slack Laboratory Animal Co., Ltd., China, and raised under standard conditions. All the experiments performed on animals were approved by the Animal Care and Use Committee of Hangzhou Medical College and implemented following the Guide for the Care and Use of Laboratory Animals (GB/T 35892–2018).
BALB/c nude mice aged 5 weeks were purchased from Shanghai Slack Laboratory Animal Co., Ltd., China, and raised under standard conditions. All the experiments performed on animals were approved by the Animal Care and Use Committee of Hangzhou Medical College and implemented following the Guide for the Care and Use of Laboratory Animals (GB/T 35892–2018).
10
Gastric Cancer Cell Line Characterization
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GC cells (AGS, HGC-27, BGC-823, MKN-45, MKN-74, and SGC-7901) and human gastric mucosal epithelial cells (GES-1) were purchased from the Beijing Institute of Cancer Research (Beijing, China). HEK-293T cells were purchased from Procell Biotech (Wuhan, China). GC cells and HEK-293T cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco Company, USA) containing 10% fetal bovine serum (FBS) 100 unit/mL penicillin and 100 g/mL streptomycin in a humidified atmosphere at 37 °C and 5% CO2. The GES-1 cell was cultured in RPMI-1640 medium (Gibco Company, USA) mixed with 10% FBS. In this experiment, antibodies used as follows, PSMA1(ab109530, Abcam), PSMA1(sc-166073, Santa Cruz), YAP (#4912, CST), YAP (13584-1-AP, Proteintech), TAZ (#83669, CST), TAZ (23306-1-AP, Proteintech), Ki67 (ab1667, Abcam), ACTIN (20536-1-AP, Proteintech), FLAG (20543-1-AP, Proteintech), MYC (#2276, CST), HA (51064-2-AP, Proteintech), PCNA (10205-2-AP, Proteintech), and C-Myc (10828-1-AP, Proteintech).
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