Amazon sl

Manufactured by Bruker

Sourced in Germany, United States

The AmaZon SL is a high-performance liquid chromatography mass spectrometry (LC-MS) system designed for a wide range of analytical applications. It features a robust and reliable ion trap mass analyzer that provides accurate mass measurements and high-quality data. The AmaZon SL is capable of performing various types of mass spectrometry analyses, including full-scan, targeted, and data-dependent acquisition modes.

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Close spelling variants of this product

AmaZon SL ion trap mass spectrometer (43 citations) AmaZon SL mass spectrometer (16 citations) Model Amazon SL (11 citations) Amazon SL ion trap (9 citations) Amazon-SL quadrupole ion trap mass spectrometer (5 citations) AmaZon SL ion trap MS (3 citations) AmaZon SL spectrometer (3 citations) Amazon SL Ion Trap mass spectrometry (3 citations) AmaZon SL-NPC (2 citations) AmaZon SL system (2 citations)

62 protocols using amazon sl

LC-MS/MS Quantification of Drugs in Plasma and Tissue

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Chromatographic conditions. Plasma and tissue samples from all drug-treated animals at selected time points were analyzed using a previously developed nonvalidated LC–MS/MS method. A sensitive and highly selective liquid chromatography–tandem mass spectrometry (LC–MS) method was used to determine the drug concentration in mouse plasma samples or tissue homogenates. The LC–MS analysis was carried out on a Bruker amaZon SL mass spectrometer using the positive/negative ion ESI mode. Chromatographic separation was achieved on an Ascentis Express C18 column (5 cm × 2.1 mm, 2.7 μM, Supelco Technologies) at room temperature with a thermostatted column oven. A gradient elution of eluents A (acetonitrile (LiChrosolv, Reag. Ph Eur) + 0.1% formic acid (Sigma Aldrich, 98–100%)) and B (water + 0.1% formic acid) were used for separation. The flow rate was set at 1 mL/min. The injection volume was 20 μL, and the time of injection was 4 min.
Mass spectrometric conditions. An ion trap mass spectrometer (Bruker amaZon SL, Bruker, Bremen, Germany) was equipped with an electrospray source operating in the positive/negative ion mode. Data were collected and processed using Bruker Quant Analysis (Bruker, Bremen, Germany) software. A quantification of the analytes was performed in the SIM mode.

Kaczorowska K., Stankiewicz A., Bugno R., Paluchowska M.H., Burnat G., Brański P., Cieślik P., Wierońska J.M., Milik M., Nowak M., Przybyłowicz A., Kozioł A., Hogendorf A., Hogendorf A.S., Kalinowska-Tłuścik J., Duszyńska B., Pilc A, & Bojarski A.J. (2023). Design and Synthesis of New Quinazolin-4-one Derivatives with Negative mGlu7 Receptor Modulation Activity and Antipsychotic-Like Properties. International Journal of Molecular Sciences, 24(3), 1981.

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Characterization of Au(kin)(PPh3) Complex

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The [Au(kin)(PPh₃)] complex (1) was characterized using CHN elemental analyses (CHN analyser Flash Smart CHN, Thermo Scientific, Waltham, MA, USA), infrared spectroscopy (IR) performed using an ATR technique and in the range of 400–4000 cm⁻¹ (Nicolet iS5 FT–IR, Thermo Nicolet), TG/DSC thermogravimetric analyses in the temperature range of 23 °C to 900 °C (Netzsch STA 449 F1 Jupiter^®, Bayern, Germany), and mass spectrometry (MS) performed using the ESI+ technique (Bruker amaZon SL, Bruker, Billerica, MA, USA). ¹H, ¹³C, ¹⁵N, and ³¹P NMR spectra were recorded using a JEOL JNM-ECA 600II (Tokyo, Japan) device on the DMF-d₇ solutions at 300 K and single crystal X-ray analysis preformed using a D8 Quest diffractometer (Bruker, Billerica, MA, USA) at 293 K.

Trávníček Z., Vančo J., Belza J., Hošek J., Dvořák Z., Lenobel R., Popa I., Šmejkal K, & Uhrin P. (2023). The Gold(I) Complex with Plant Hormone Kinetin Shows Promising In Vitro Anticancer and PPARγ Properties. International Journal of Molecular Sciences, 24(3), 2293.

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Quantification of Phytochemicals in Plant Extracts

Cited 1 time

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Chemical analyses were done as previously described [12 (link),13 (link)]. Phenolic compounds were analysed by high pressure liquid chromatography (HPLC) (Agilent HPLC series 1100, Agilent Technologies Sales & Services GmbH & Co. KG, Waldbronn, Germany) coupled to an ion trap mass spectrometer (Bruker Amazon SL, Bruker, Bremen, Germany). Quantification of phenolics was done using standards of caffeoylquinic acid [chlorogenic acid], quercetin 3-O-glucoside, kaempferol 3-O glucoside and isorhamnetin-3-O-glucoside (Carl Roth, Karlsruhe, Germany), which were used for external calibration curves. Carotenoids and chlorophylls were analysed using ultra-high-pressure liquid chromatography (UHPLC) (Agilent Technologies 1290 Infinity II) coupled with time of flight mass spectrometry (ToF-MS) (Agilent Technologies 6230 TOF LC/MS) equipped with an atmospheric pressure chemical ionization source (Agilent Technologies). External standard calibration curves of each compound were used for quantification. Compounds below the quantification limit were indicated as not detected (n.d.).

Odongo G.A., Schlotz N., Baldermann S., Neugart S., Huyskens-Keil S., Ngwene B., Trierweiler B., Schreiner M, & Lamy E. (2018). African Nightshade (Solanum scabrum Mill.): Impact of Cultivation and Plant Processing on Its Health Promoting Potential as Determined in a Human Liver Cell Model. Nutrients, 10(10), 1532.

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Synthesis and Evaluation of Sulfonamide Inhibitors

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Commercially available reagents were used without additional purification unless otherwise stated. 4,5-dichlorophthalic anhydride, 4-aminobenzenesulfonamide, and 4-(2-aminoethyl)benzenesulfonamide were purchased from Sigma-Aldrich (Sydney, Australia). ¹H-NMR (400 MHz) and ¹³C-NMR (101 MHz) spectroscopy was obtained using a Gemini 400-MR NMR spectrometer (Varian, Palo Alto, CA, USA), using DMSO-d₆ as NMR solvent. Low-resolution ESI-MS was measured on an amaZon SL (Bruker Daltonics, Bremen, Germany). High resolution ESI-MS was performed on an Apex Ultra Fourier Transform Ion Cyclotron Resonance 7 T Mass Spectrometer (Bruker Daltonics, Bremen, Germany). The mass spectra were obtained by direct infusion electrospray ionization and are reported as mass to charge ratio (m/z) and relative intensity (%). The estimated purity for the synthesised inhibitors is > 93%, with the final yield approximately 70%. HPLC was performed using a CBM-20A Shimadzu HPLC system (Kyoto, Japan), with an Ascentis C18 HPLC column (particle size: 10 μm, length: 25 cm, internal diameter: 4.6 mm) purchased from Sigma-Aldrich (Sydney, Australia). The inhibition assay data was modelled with a nonlinear regression fit of the log[inhibitor] vs. normalised response (using a variable slope) in GraphPad Prism v7.02 software [40 ], and the IC₅₀ is reported with 95% confidence intervals.

Jayawickrama G.S., Nematollahi A., Sun G, & Church W.B. (2018). Improvement of kynurenine aminotransferase-II inhibitors guided by mimicking sulfate esters. PLoS ONE, 13(4), e0196404.

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Identification of Antioxidant Peptides

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The principal subfractions (enriched peptides) obtained from the RP-HPLC fractionation that showed a marked NO radical scavenging activity and a sufficient yield (F_2-1 to F_2-3 and F_2-5) were identified by amino acid sequencing using quadrupole time-of-flight (Q-TOF) liquid chromatography-tandem mass spectrometry (Q-TOF LC-MS/MS) coupled with electrospray ionization (ESI; model Amazon SL, Bruker, Bremen, Germany). The MS/MS data were searched against the Swiss-Prot database with the MASCOT package (www.matrixscience.com) (23 (link)).

Inkanuwat A., Sukaboon R., Reamtong O., Asawanonda P., Pattaratanakun A., Saisavoey T., Sangtanoo P, & Karnchanatat A. (2019). Nitric Oxide Synthesis Inhibition and Anti-Inflammatory Effect of Polypeptide Isolated from Chicken Feather Meal in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages. Food Technology and Biotechnology, 57(2), 200-212.

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Compound Identification Using UFLC-MS

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To suggest the identification of compounds related to the major peaks, UFLC (Shimadzu, Japan) coupled with a photodiode array detector (SPD-M20A) and equipped with an amaZon SL (Bruker Daltonics, Germany) mass spectrometer with an electron spray ionization and ion trap instrument was employed. The column used was a C18 Hypersil GOLD (5 μm)—Thermo Scientific, 4.5 x 250 mm, and the guard cartridge consisted of the same stationary phase. The mobile phase consisted of solvent A (HPLC grade water and 1% formic acid) and solvent B (HPLC grade acetonitrile and 0.1% formic acid) and was run under gradient conditions following the program: 7, 15, 20, 20, 35, and 7% solvent B at 0, 7, 25, 35, 42, and 45 min, respectively. The flow rate was 1 mL/min. The run time was 50 min, and the detection was carried out at 320 nm.

Alves G.D., Oliveira de Souza R., Ghislain Rogez H.L., Masaki H, & Fonseca M.J. (2019). Cecropia obtusa extract and chlorogenic acid exhibit anti aging effect in human fibroblasts and keratinocytes cells exposed to UV radiation. PLoS ONE, 14(5), e0216501.

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Spectroscopic Characterization of Organic Compounds

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The chemicals and instruments Reagents were purchased from Aldrich unless specified otherwise. All solvents were purified and degassed prior to use. NMR spectra were recorded on a Bruker FT-NMR Avance III 500 MHz instrument at 500.32 (1H). Chemical shifts were referenced relative to the solvent signal for 1H. ESI mass spectra were recorded on an LC/MSn ion trap mass spectrometer amaZon SL (Bruker, Bremen, Germany) with MeOH as a solvent. Elemental analysis was performed at Moscow State University with MicroCube Elementar analyzer.

Kasparkova J., Kostrhunova H., Novohradsky V., Ma L., Zhu G., Milaeva E.R., Shtill A.A., Vinck R., Gasser G., Brabec V, & Nazarov A.A. (2022). Is antitumor Pt(IV) complex containing two axial lonidamine ligands a true dual- or multi-action prodrug?. Metallomics : integrated biometal science, 14(7).

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HPLC-MS Analysis of Phytochemicals

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The ROe was analyzed according to a previously described method (Couto et al. 2011) , in which a HPLC Prominence Shimadzu ® coupled to a mass spectrometer amaZon SL Bruker Daltonics ® was used. The HPLC system comprised a LC-20AD pump, DGU 20-A3R online degassing unit, SPD-M20A diode array detector, CTO-20A column oven, SIL-20AHT automatic injector and CBM-20 communication module. The dried extracts were solubilized in ACN:H 2 O (8:2, v/v) at a concentration of 1.0 mg/mL, centrifuged for 3 min at 10000 g in an Eppendorf ® Minispin centrifuge. Thereafter, the supernatant was analyzed. Chromatographic separations were performed in a Phenomenex ® C 18 -Luna column (250 x 4.6 mm i.d., 5 μm). The injection volume was 2 µL. The eluent system consisted in H 2 O (A) and ACN (B), both acidified with 0.1% v/v formic acid, and we used a linear gradient from 5 to 100% B in 40 min at a flow rate of 1.0 mL/ min. The oven temperature was set at 25 ˚C. The mass spectra were obtained separately in both positive and negative mode, in a mass range of 50-1200 Da and applying auto-MSn (n = 3) mode. The mass spectrometer source parameters were set as follows: capillary voltage at 4.5 V, nitrogen used as the nebulizing and drying gas (50 psi, 10 L min -1 , 300 °C). The data was processed through Bruker Compass Data Analysis 4.3 ® software.

Takayama K.S., Monteiro M.C., Saito P., Pinto I.C., Nakano C.T., Martinez R.M., Thomaz D.V., Verri WA J.r., Baracat M.M., Arakawa N.S., Russo H.M., Zeraik M.L., Casagrande R., Couto R.O.D, & Georgetti S.R. (2022). Rosmarinus officinalis extract-loaded emulgel prevents UVB irradiation damage to the skin. Anais da Academia Brasileira de Ciencias, 94(4).

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Ion trap mass spectrometry protocol

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An ion trap mass spectrometer (Bruker amaZon SL) was equipped with an electrospray source operating in positive/negative ion mode. Data were collected and processed using Bruker Quant Analysis software. Quantification of the analytes was performed in SIM mode.

Stankiewicz A., Kaczorowska K., Bugno R., Kozioł A., Paluchowska M.H., Burnat G., Chruścicka B., Chorobik P., Brański P., Wierońska J.M., Duszyńska B., Pilc A, & Bojarski A.J. (2021). New 1,2,4-oxadiazole derivatives with positive mGlu4 receptor modulation activity and antipsychotic-like properties. Journal of Enzyme Inhibition and Medicinal Chemistry, 37(1), 211-225.

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Sequencing Antioxidant Peptide Fractions

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The peptide fraction isolated from RP-HPLC that showed the highest antioxidant activity (F₄) was subjected to Q-TOF- -MS/MS coupled with electrospray ionization (ESI; model Amazon SL, Bruker, Germany) in order to sequence the main components de novo. The MS/MS data were submitted for a database search against the Swiss-Prot database using the Mascot package (17 (link)).

Suttisuwan R., Phunpruch S., Saisavoey T., Sangtanoo P., Thongchul N, & Karnchanatat A. (2019). Free Radical Scavenging Properties and Induction of Apoptotic Effects of Fa Fraction Obtained after Proteolysis of Bioactive Peptides from Microalgae Synechococcus sp. VDW. Food Technology and Biotechnology, 57(3), 358-368.

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