U 25nd25
The U-25ND25 is a neutral density filter designed for use with Olympus microscopes. It reduces the intensity of light passing through the optical system, allowing for more precise control of illumination and improved image quality.
Lab products found in correlation
6 protocols using u 25nd25
Characterization of Tribofilm Nanostructures
Quantifying Autophagic Induction via MDC
Intracellular Nrf2 Localization by Immunofluorescence
Immunofluorescent Analysis of Lung Tissue
Intracellular Nrf2 Nucleoprotein Quantification
Optogenetic Neural Stimulation Protocol
Stimulation of optogenetic probes LED/mercury light stimulation Wide-field optical stimulation was achieved by high power LEDs (M470L2 and M590L3 for ReaChR and CsChrimson, respectively, Thorlabs) or filtered light from a mercury lamp (for Arch). An LED (M470L2) with peak output at wavelength of $470 nm was used for both the activation of ReaChR and excitation of Citrine tagged to ReaChR. Because ReaChR is sensitive to a broad spectrum of light (Lin et al., 2013) , blue light was sufficient to make the PNs expressing ReaChR fire at $200 Hz (Figure S3A). Light from an LED or a mercury lamp was collimated and delivered to an upright microscope (BX51Wl, Olympus) equipped with a 40x water-immersion objective lens (NA 0.80). LED light was pulsated at 80 Hz. Neutral-density filters (U-25ND25 or U-25ND6, Olympus) were used to stimulate the cells at lower intensities. All the reported optical intensity of LED light was measured at the back aperture of the objective lens (S120VC sensor, Thorlabs).
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