Stat3fl fl, by Jackson ImmunoResearch - Product details

1

Genetically Modified Mouse Models for Immunological Studies

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C57BL/6J (B6),
Prdm1^fl/flFoxP3^YFP-Cre(FoxP3^Cre),
Rosa26^Cre-ERT2 (iCre⁺),
Bol6^fl/fl,
Stat3^fl/fl,
Tcrα^−/−(Jackson
Labs),
Rag₂^−/−,
B6SJL (CD45.1) (Taconic Farms), and B6.FoxP3-GFP reporter mice were housed
in pathogen-free conditions. Prdm1^fl/fl mice
were bred onto FoxP3^Cre or
Rosa26^Cre-ERT2 (iCre⁺) mice to
generate
Prdm1^fl/flFoxP3^Cre,
Prdm1^fl/+FoxP3^Cre,
or
Prdm1^fl/flRosa26^Cre-ERT2(Prdm1^fl/fliCre⁺)
mice, respectively. Bol6^fl/fl mice were bred
onto FoxP3^Cre to generate
Bol6^fl/flFoxP3^Cremice that were further crossed onto
Prdm1^fl/flFoxP3^Creto yield
Bol6^fl/flPrdm1^fl/flFoxP3^Cremice. Sfaf3^fl/fl mice were crossed onto
Prdm1^fl/flFoxP3^Creto yield
Stat3^fl/+Prdm1^fl/flFoxP3^Creor
Stat3^fl/flPrdm1^fl/flFoxP3^Cremice. All mice were used at the age of 6 to 9 weeks unless otherwise
specified. Both sexes (males or females) were randomly included for all
experiments in an unblinded fashion. Generally between 3 to 7 mice were used
per group, as indicated in each experiment. All experiments were performed
in compliance with federal laws and institutional guidelines as approved by
DFCI’s and UAB’s Animal Care and Use Committee.

Shen E., Rabe H., Luo L., Wang L., Wang Q., Yin J., Yang X., Liu W., Sido J.M., Nakagawa H., Ao L., Kim H.J., Cantor H, & Leavenworth J.W. (2019). Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell reports, 29(7), 1848-1861.e6.

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2

Conditional Genetic Modification of Mice

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C57BL/6 (B6), BALB/c, Cd4-Cre, Mtor^fl/fl, and Stat3^fl/fl mice were purchased from Jackson Laboratory (Bar Harbor, MA). A further description of these mouse strains is listed in Supplementary Table S1. Mtor^fl/fl and Stat3^fl/fl mice were crossed with Cd4-Cre mice to create Mtor^fl/flCd4-Cre, Stat3^fl/flCd4-Cre, and Mtor^fl/flStat3^fl/flCd4-Cre mice. Mice were housed in a specific pathogen free facility at Houston Methodist Research Institute in Houston, Texas. All animal experiments in this study were approved by the Houston Methodist Animal Care Committee in accordance with institutional animal care and use guidelines.

Xie A., Yan H., Fu J., He A., Xiao X., Li X.C, & Chen W. (2019). T follicular helper and memory cell responses and the mTOR pathway in murine heart transplantation. The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation, 39(2), 134-144.

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3

Mouse Models for Gut Microbiome Studies

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C57BL/6, 7B8Tg, zDC-DTR, CCR7^−/−, CCR2^−/−, BATF3^−/−, CX3CR1^gfp/gfp, IL-6^−/−, MHCII^fl/fl, STAT3^fl/fl, IL-6Rα^fl/fl, CD4^Cre, Lysm^Cre, CD11c^Cre mice were purchased from The Jackson Laboratory. Mice were bred and maintained at the specific pathogen free animal facilities of the Medical University of South Carolina and later at the Ohio State University. All experiments were performed under protocols approved by Institutional Animal Care and Use Committee at both Universities. Mouse colonies were screened regularly for the presence of fecal SFB. Mice contaminated with SFB was treated with ampicillin in drinking water (1g/ml) for 2 weeks and let recovered before being used for experiments. SFB donor mice include C57BL/6 mice sourced from Taconic and in house SFB⁺ mice.

Ngoi S., Yang Y., Iwanowycz S., Gutierrez J., Li Y., Williams C., Hill M., Chung D., Allen C, & Liu B. (2022). Migrating Type 2 Dendritic Cells Prime Mucosal Th17 Cells Specific to Small Intestinal Commensal Bacteria. Journal of immunology (Baltimore, Md. : 1950).

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4

Genetically Engineered Mouse Models for Immunology Research

Cited 1 time

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C57BL/6 WT, MyD88^−/− and Il1r1^−/− mice on C57BL/6 background were purchased from the Jackson Laboratory. IL-23R KO mice were imported from Genetech. hCD2-Cre mice (Jackson Laboratory) were crossed with Raptor^fl/fl mice, Rictor^fl/fl mice (Jackson Laboratory) and Stat3^fl/fl mice (Ding et al., 2016 (link)) to generate CD2-cre;Raptor^fl/fl, CD2-cre;Rictor^fl/fl and CD2-cre;Stat3^fl/fl conditional KO (cKO) mice. Male and female mice were used in all experiments (6–12 weeks old) and were housed in specific pathogen free conditions. All experiments were in accordance with institutional guidelines and approved by the IACUC at the University of Louisville.

Cai Y., Xue F., Qin H., Chen X., Liu N., Fleming C., Hu X., Zhang H.G., Chen F., Zheng J, & Yan J. (2019). Differential Roles of the mTOR-STAT3 Signaling in Dermal γδ T Cell Effector Function in Skin Inflammation. Cell reports, 27(10), 3034-3048.e5.

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5

Genetically Modified Mouse Models for Immunological Studies

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C57BL/6J (B6),
Prdm1^fl/flFoxP3^YFP-Cre(FoxP3^Cre),
Rosa26^Cre-ERT2 (iCre⁺),
Bol6^fl/fl,
Stat3^fl/fl,
Tcrα^−/−(Jackson
Labs),
Rag₂^−/−,
B6SJL (CD45.1) (Taconic Farms), and B6.FoxP3-GFP reporter mice were housed
in pathogen-free conditions. Prdm1^fl/fl mice
were bred onto FoxP3^Cre or
Rosa26^Cre-ERT2 (iCre⁺) mice to
generate
Prdm1^fl/flFoxP3^Cre,
Prdm1^fl/+FoxP3^Cre,
or
Prdm1^fl/flRosa26^Cre-ERT2(Prdm1^fl/fliCre⁺)
mice, respectively. Bol6^fl/fl mice were bred
onto FoxP3^Cre to generate
Bol6^fl/flFoxP3^Cremice that were further crossed onto
Prdm1^fl/flFoxP3^Creto yield
Bol6^fl/flPrdm1^fl/flFoxP3^Cremice. Sfaf3^fl/fl mice were crossed onto
Prdm1^fl/flFoxP3^Creto yield
Stat3^fl/+Prdm1^fl/flFoxP3^Creor
Stat3^fl/flPrdm1^fl/flFoxP3^Cremice. All mice were used at the age of 6 to 9 weeks unless otherwise
specified. Both sexes (males or females) were randomly included for all
experiments in an unblinded fashion. Generally between 3 to 7 mice were used
per group, as indicated in each experiment. All experiments were performed
in compliance with federal laws and institutional guidelines as approved by
DFCI’s and UAB’s Animal Care and Use Committee.

Shen E., Rabe H., Luo L., Wang L., Wang Q., Yin J., Yang X., Liu W., Sido J.M., Nakagawa H., Ao L., Kim H.J., Cantor H, & Leavenworth J.W. (2019). Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell reports, 29(7), 1848-1861.e6.

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6

Murine Models for Cancer Research

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Wild-type C57BL/6J mice and breeding pairs of Apc^Min^/+, Gfap-tk (thymidine kinase), Gfap-Cre, Plp1-CreER^T, inducible diphtheria toxin receptor (iDTR), Rag2^-/- γc^-/-, Myd88^fl/fl, Ifngr1^fl/fl, Rela^fl/fl, and Stat3^fl/fl mice were purchased from The Jackson Laboratory. All mice were housed in an American Association for the Accreditation of Laboratory Animal Care-accredited animal facility and maintained in specific pathogen-free conditions on wood-chip bedding and fed standard rodent chow ad libitum unless otherwise stated. Littermates were used for controls whenever possible; if different litters had to be combined, mice were age matched and non-littermates were co-housed to eliminate effects of the microbiome. Mice of both sexes were utilized in the experiments performed in this study, and experiments were carried out during the light cycle. All animal care and experimentation were approved by the Stanford University Institutional Animal Care and Use Committee.

Yuan R., Bhattacharya N., Kenkel J.A., Shen J., DiMaio M.A., Bagchi S., Prestwood T.R., Habtezion A, & Engleman E.G. (2020). Enteric Glia Play a Critical Role in Promoting the Development of Colorectal Cancer. Frontiers in Oncology, 10, 595892.

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7

Genetically Engineered Mouse Models for Immunology Research

Cited 1 time

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C57BL/6 WT, MyD88^−/− and Il1r1^−/− mice on C57BL/6 background were purchased from the Jackson Laboratory. IL-23R KO mice were imported from Genetech. hCD2-Cre mice (Jackson Laboratory) were crossed with Raptor^fl/fl mice, Rictor^fl/fl mice (Jackson Laboratory) and Stat3^fl/fl mice (Ding et al., 2016 (link)) to generate CD2-cre;Raptor^fl/fl, CD2-cre;Rictor^fl/fl and CD2-cre;Stat3^fl/fl conditional KO (cKO) mice. Male and female mice were used in all experiments (6–12 weeks old) and were housed in specific pathogen free conditions. All experiments were in accordance with institutional guidelines and approved by the IACUC at the University of Louisville.

Cai Y., Xue F., Qin H., Chen X., Liu N., Fleming C., Hu X., Zhang H.G., Chen F., Zheng J, & Yan J. (2019). Differential Roles of the mTOR-STAT3 Signaling in Dermal γδ T Cell Effector Function in Skin Inflammation. Cell reports, 27(10), 3034-3048.e5.

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8

Intestinal STAT3 Knockout Mouse Model

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Mice with a floxed STAT3 allele (Stat3^fl/fl; Stock No. 016903) were purchased from the Jackson Laboratory (Bar Harbor, ME). Intestinal epithelial cell (IEC)-specific STAT3 (Stat3^IEC-/-) mice were generated by breeding corresponding floxed mice with Villin997-Cre mice (the Jackson Laboratory; Stock No. 004586). Wild type (WT) C57BL/6J mice (Stock No. 000664) were also obtained from the Jackson Laboratory. All mice were kept in ventilated cages under specific pathogen-free condition. Animal experiments were approved by the Institutional Animal Care and Use Committee of North Carolina Research Campus (Protocol #21-008).

Yue R., Wei X., Hao L., Dong H., Guo W., Sun X., Zhao J., Zhou Z, & Zhong W. (2023). Promoting intestinal antimicrobial defense and microbiome symbiosis contributes to IL-22-mediated protection against alcoholic hepatitis in mice. Frontiers in Immunology, 14, 1289356.

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9

Multiparametric Mouse Immune Phenotyping

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Itga3^fl/fl (#008818), Il17a^Cre/+ (#016879), CD4-Cre (#022071), Rosa26^{lsl-Zsgreen/WT} (#007906), 2D2 TCR transgenic (#006912), CD45.1⁺ congenic (#002014), Stat3^fl/fl (#016923), and Tcra KO (#002116) mice were obtained from the Jackson Laboratory. Mice were bred and maintained in specific-pathogen-free facilities at Duke University and used in accordance with the Duke Institutional Animal Care and Use Committee guidelines. Experimental mice were used between 8 and 14 weeks of age with no preference for sex, except for adoptive transfer of CD4⁺ T cells, where donors and recipients were sex-matched. Co-housed experimental mice and littermate controls were used.

Jüppner H., Schipani E., Bastepe M., Cole D.E., Lawson M.L., Mannstadt M., Hendy G.N., Plotkin H., Koshiyama H., Koh T., Crawford J.D., Olsen B.R, & Vikkula M. (1998). The gene responsible for pseudohypoparathyroidism type Ib is paternally imprinted and maps in four unrelated kindreds to chromosome 20q13.3. Proceedings of the National Academy of Sciences of the United States of America, 95(20), 11798-11803.

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Stat3fl fl

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9 protocols using stat3fl fl

Genetically Modified Mouse Models for Immunological Studies

Conditional Genetic Modification of Mice

Mouse Models for Gut Microbiome Studies

Genetically Engineered Mouse Models for Immunology Research

Genetically Modified Mouse Models for Immunological Studies

Murine Models for Cancer Research

Genetically Engineered Mouse Models for Immunology Research

Intestinal STAT3 Knockout Mouse Model

Multiparametric Mouse Immune Phenotyping

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