KF was obtained from Kwangmyungdang Medical Herbs (Ulsan, Republic of Korea). Ground KF (100 g) was soaked in 1 L of methanol. After sonication for 30 min, the mixture was kept at room temperature for 48 h. The supernatant was collected and filtered (No. 20 Whatman; GE Healthcare, Chalfont, UK). To derive a lyophilized powder from the filtrate, evaporation using a vacuum evaporator (Eyela; Tokyo Rikakikai, Tokyo, Japan) and lyophilization using a freezer dryer (FreeZone 6 Liter Console Freeze Dry System; Labconco, Kansas City, MO, USA) were performed sequentially. Finally, 4.5 g of powder was obtained for a yield of 4.45%. The powder was dissolved in dimethyl sulfoxide (DMSO) and applied to the cells.
Freezone 6 liter console freeze dry system
Manufactured by Labconco
Sourced in United States
The FreeZone 6 Liter Console Freeze Dry System is a compact, self-contained freeze dryer designed for laboratory use. It features a 6-liter collector capacity and can achieve temperatures down to -50°C. The system is equipped with a control panel for monitoring and adjusting the freeze drying process.
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Lab products found in correlation
2 protocols using freezone 6 liter console freeze dry system
1
KF Extract Preparation and Application
2
Antifungal Assay on Apple Agar
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For assays in solid media, apple agar was prepared using lyophilized apple powder (30 g/L) and bacteriological agar (20 g/L). Apple powder was achieved by cutting apples in pieces, froze them at -80 C for at least 3h and lyophilizing the pieces in a Bulk Tray Dryer with a 6-Port Manifold coupled to a FreeZone 6 Liter Console Freeze Dry System (Labconco, USA). The lyophilized pieces were then pulverized in a crusher. After dissolving these ingredients by stirring, the medium was sterilised at 121 ºC for 16 min.
To obtain the three batches with different PgAFP concentrations 0, 10 and 40 µg/mL, melted apple agar (45 ºC) was supplemented with a sterile solution of PgAFP prior to being poured into Petri plates. They were stored at room temperature until agar solidification. Then, plates were centrally inoculated with 2 µL from a spore suspension of P. expansum CMP-1 (10 6 conidia/mL) and incubated for 15 days at 25 ºC.
To obtain the three batches with different PgAFP concentrations 0, 10 and 40 µg/mL, melted apple agar (45 ºC) was supplemented with a sterile solution of PgAFP prior to being poured into Petri plates. They were stored at room temperature until agar solidification. Then, plates were centrally inoculated with 2 µL from a spore suspension of P. expansum CMP-1 (10 6 conidia/mL) and incubated for 15 days at 25 ºC.
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