Treadscan

Manufactured by CleverSys

Sourced in United States

TreadScan is a laboratory equipment designed to analyze the physical characteristics of treadmill surfaces. It utilizes advanced sensors to precisely measure and record various parameters, such as surface texture, friction, and elasticity. The core function of TreadScan is to provide accurate and objective data to support research, product development, and quality assurance in the field of treadmill and exercise equipment manufacturing.

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Lab products found in correlation

Neurodegenscan suite, by CleverSys (1 mentions)

13 protocols using treadscan

Gait Analysis of Mice Using TreadScan

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TreadScan (CleverSys Inc., Reston, VA) is an unbiased device used for gait analysis of animals. All mice were allowed to walk on the motor-driven treadmill belt at a speed of 11 cm/s for a period of 20 s as described previously (Beare et al., 2009 (link)). The digital data (footprints and body movement) were analyzed by a TreadScan software (CleverSys Inc., Reston, VA), and each of the parameters was compared between ipsilateral and contralateral sides and between the treatment and control groups.

Wang Y., Wu W., Wu X., Sun Y., Zhang Y.P., Deng L.X., Walker M.J., Qu W., Chen C., Liu N.K., Han Q., Dai H., Shields L.B., Shields C.B., Sengelaub D.R., Jones K.J., Smith G.M, & Xu X.M. (2018). Remodeling of lumbar motor circuitry remote to a thoracic spinal cord injury promotes locomotor recovery. eLife, 7, e39016.

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Gait Analysis of Transgenic Mice

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Gait testing was performed using a TreadScan (CleverSys, Reston, VA, USA) digital analysis system. Mice walked on a transparent treadmill belt kept at a speed of 5 cm/s and were imaged from below using a high‐speed digital camera. A total of five videos, 800 frames per video, were collected per day for each mouse and videos were analysed using TreadScan software so as to get the following gait characteristics: stance time [milliseconds (ms) that the is paw on the belt], swing time (ms that the paw is in the air), and total stride length [millimeters (mm)]. The tester was blinded to the genotype of the mice.

Svensson K., LaBarge S.A., Sathe A., Martins V.F., Tahvilian S., Cunliffe J.M., Sasik R., Mahata S.K., Meyer G.A., Philp A., David L.L., Ward S.R., McCurdy C.E., Aslan J.E, & Schenk S. (2020). p300 and cAMP response element‐binding protein‐binding protein in skeletal muscle homeostasis, contractile function, and survival. Journal of Cachexia, Sarcopenia and Muscle, 11(2), 464-477.

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Automated Gait Analysis in Mice

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Mouse gait was assessed using the CleverSys TreadScan for forced gait analysis. The TreadScan apparatus consists of a treadmill chamber placed above a transparent belt treadmill illuminated by external light sources. A high-speed digital camera is placed underneath the treadmill chamber to capture ventral videos of mice on the treadmill. Mice were placed into the treadmill chamber and allowed to freely explore for 1 minute. The treadmill was turned on and off at a speed of 20 cm/s until the test mouse was continuously running. A 20-second video was then captured for footprint placement and movement analysis. Video files were automatically segmented using TreadScan software to include only periods of continual running. Video files were additionally manually clipped to eliminate periods when the mouse slowed running before a stop or exhibited lateral movement. This list of segments was subjected to footprint analysis with paw identification training modules built in accordance with the manufacturer’s instructions. All analyzed gait metrics were weighted by number of counted steps per foot.

Collins B.E., Merritt J.K., Erickson K.R, & Neul J.L. (2021). Safety and efficacy of genetic MECP2 supplementation in the R294X mouse model of Rett syndrome. Genes, Brain, and Behavior, 21(1), e12739.

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Gait Analysis in Gfa2-CGG99 Mouse Model

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Gait abnormalities in Gfa2-CGG99 mice were analyzed and compared to WT mice using a motor-driven transparent treadmill affixed with high-speed digital video camera and computer-assisted software (TreadScan, CleverSys, Reston,VA). To ensure that all mice were walking, and not galloping, the treadmill speed was adjusted for each mouse but never exceeded 20 cm/s. When the mouse reached a steady gate, video of the underside was recorded and analyzed by the system’s software that identified each paw individually and calculated gait variables. Variables included stride time, defined as the time between two initial paw contacts of the same paw, stance time, the period of time the paw is in contact with the treadmill, and swing time, the portion of the stride in which the paw is not in contact with the treadmill.

Wenzel H.J., Murray K.D., Haify S.N., Hunsaker M.R., Schwartzer J.J., Kim K., La Spada A.R., Sopher B.L., Hagerman P.J., Raske C., Severijnen L.A., Willemsen R., Hukema R.K, & Berman R.F. (2019). Astroglial-targeted expression of the fragile X CGG repeat premutation in mice yields RAN translation, motor deficits and possible evidence for cell-to-cell propagation of FXTAS pathology. Acta Neuropathologica Communications, 7, 27.

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Automated Gait Analysis in Mice

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To assess motor function and coordination in walking mice, automated gait analysis was performed using Treadscan (Cleversys Inc, Reston, VA). Gait patterns of 3–6 mo mice were measured for 25 sec at a speed of 13 cm/sec on a transparent running belt illuminated by a LED light and reflecting footprints captured by a video camera positioned underneath the walkway.

Nuber S., Rajsombath M., Minakaki G., Winkler J., Müller C.P., Ericsson M., Caldarone B., Dettmer U, & Selkoe D.J. (2018). Abrogating native α-synuclein tetramers in mice causes a L-DOPA responsive motor syndrome closely resembling Parkinson’s disease. Neuron, 100(1), 75-90.e5.

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Quantitative Analysis of Mouse Gait

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Mouse gait was assessed using the CleverSys TreadScan for forced gait analysis. The TreadScan apparatus consists of a treadmill chamber placed above a transparent belt treadmill illuminated by external light sources. A high‐speed digital camera is placed underneath the treadmill chamber to capture ventral videos of mice on the treadmill. Mice were placed into the treadmill chamber and allowed to freely explore for 1 min. The treadmill was turned on and off at a speed of 20 cm/s until the test mouse was continuously running. A 20‐s video was then captured for footprint placement and movement analysis. Video files were automatically segmented using TreadScan software to include only periods of continual running. Video files were additionally manually clipped to eliminate periods when the mouse slowed running before a stop or exhibited lateral movement. This list of segments was subjected to footprint analysis with paw identification training modules built in accordance with the manufacturer's instructions. All analyzed gait metrics were weighted by number of counted steps per foot.

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Automated Gait Analysis in Mice

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To assess motor function and coordination in walking mice, automated gait analysis was performed using Treadscan (Cleversys Inc, Reston, VA). Gait patterns of 3–6 mo mice were measured for 25 s at a speed of 13 cm/s on a transparent running belt illuminated by a LED light and reflecting footprints captured by a video camera positioned underneath the walkway.

Nuber S., Zhang X., McCaffery T.D., Moors T.E., Adom M.A., Hahn W.N., Martin D., Ericsson M., Tripathi A., Dettmer U., Svenningsson P, & Selkoe D.J. (2024). Generation of G51D and 3D mice reveals decreased α-synuclein tetramer-monomer ratios promote Parkinson’s disease phenotypes. NPJ Parkinson's Disease, 10, 47.

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Gait Cycle Analysis in Mice

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When mice spontaneously walked at a velocity of 20 cm/sec their motion was recorded by BCamCapture Version (Clever Sys Inc). TreadScan (Clever Sys Inc) was then used to analysis the standing and swing phases during gait cycles. The stand time is the time elapsed while the foot is in contact with the tread, in its stance phase. The swing time is the time elapsed while the foot is in the air, in its swing phase.

Huang G.J., Edwards A., Tsai C.Y., Lee Y.S., Peng L., Era T., Hirabayashi Y., Tsai C.Y., Nishikawa S.I., Iwakura Y., Chen S.J, & Flint J. (2014). Ectopic Cerebellar Cell Migration Causes Maldevelopment of Purkinje Cells and Abnormal Motor Behaviour in Cxcr4 Null Mice. PLoS ONE, 9(2), e86471.

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Treadmill Gait Analysis in Mice

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Gait analysis was performed using the TreadScan apparatus (CleverSys, Reston, VA). Mice were trained at a treadmill speed of 9 cm/ s, during 10 min on two consecutive days. Recordings at 63 frames/s for 30 s were acquired with the TreadScan software. Segments where the animal displayed continuous locomotion were automatically selected and analyzed using the TreadScan software. Gait analysis was performed at 9 cm/s. The gait parameters of stride, stance, and swing time were automatically calculated and average values for each hindlimb and trial were used for statistical analysis. The limb coordination parameters (phase coupling) were automatically calculated, averaging the values for each hindlimb and trial for the homologous coupling, and all four limbs for the homolateral and diagonal coupling.

Paixão S., Loschek L., Gaitanos L., Morales P.A., Goulding M, & Klein R. (2019). Identification of Spinal Neurons Contributing to the Dorsal Column Projection Mediating Fine Touch and Corrective Motor Movements. Neuron, 104(4), 749-764.e6.

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Automated Gait Analysis for Neurodegeneration

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Gait analyses were performed using the TreadScan software from the CleverSys NeurodegenScan Suite. The BcamCap image capture system records the footprints of the mice walking/running across a transparent treadmill using a high-speed camera at 100 frames per second. The track speed was started at 5.0 cm/s and slowly increased until the mouse can no longer keep pace or until the track reached 18.0 cm/s. By tracking the footprints of the animals, the system identified which foot was placed on the track and for how long by frame. The software’s video analyses gave parameters such as bilateral stance time and stride frequency, and instantaneous running speed. These data were then exported from the software into Microsoft Excel for statistical analyses by group and across time.

Spiller K.J., Khan T., Dominique M.A., Restrepo C.R., Cotton-Samuel D., Levitan M., Jafar-Nejad P., Zhang B., Soriano A., Rigo F., Trojanowski J.Q, & Lee V.M. (2018). Reduction of Matrix Metalloproteinase 9 (MMP-9) protects motor neurons from TDP-43-triggered death in rNLS8 mice. Neurobiology of disease, 124, 133-140.

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