Methanol (MEOH) MS grade and methyl tert-butyl ether (MTBE) were obtained from Sigma–Aldrich (Steinheim, Germany). Acetonitrile (ACN) MS grade (Fluka Chromassol, Spain), pyridine (Carlo Erba Reagents SAS, France), isopropanol (IPA) (Fischer, Austria), ammonium hydroxide 28% (VWR Collection Chemicals, USA) and formic acid 98% obtained from Sigma-Aldrich (St Louis, USA). Heptane MS grade, sodium hydroxide (NaOH) and C18: 0, N, O-Bis (trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane (TMCS) obtained from Sigma Aldrich, Spain. Ultra-pure water Milli-Q-Plus 185 (Millipore, USA). A mixture of standards of alkanes (Supelco, United States), methionine sulfone, O-methoxamine, a mixture of methyl acids and fatty acids (FAME C8–C22) and tricosane were obtained from Sigma Aldrich, Spain. 30 k/Daltons Centrifree protein cutoff cut filters were obtained from Millipore, United States.
Milli q plus 185
Manufactured by Merck Group
Sourced in United States, Germany, Sweden
The Milli-Q plus 185 is a water purification system designed to produce ultrapure water. It utilizes a combination of filtration, ion exchange, and UV treatment to remove contaminants and produce water of high purity.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Merck Group (7 mentions)
Hydrochloric acid (hcl), by Merck Group (4 mentions)
Trypsin, by Thermo Fisher Scientific (3 mentions)
Gfp n1, by Promega (3 mentions)
1 2 dioleoyl 3 trimetrylammonium propane chloride salt dope, by Merck Group (3 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Acetic anhydride, by Sinopharm (2 mentions)
Triethylamine, by Sinopharm (2 mentions)
Haucl4 4h2o, by Sinopharm (2 mentions)
Chloroform, by Merck Group (2 mentions)
Sylgard 184, by Dow (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Tannic acid, by Merck Group (2 mentions)
Calcium chloride dihydrate, by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Regenerated cellulose dialysis membranes, by Thermo Fisher Scientific (2 mentions)
Acetone, by Merck Group (2 mentions)
47 protocols using milli q plus 185
1
Metabolite Extraction and Derivatization
2
Metabolite Extraction and Derivatization
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Methanol (LC-MS grade), heptane (GC-MS grade), chloroform (MS grade), acetonitrile (LC-MS grade), isopropanol (LC-MS grade), formic acid (MS grade), pyridine (silylation grade), C18:0 methyl ester and O-methoxyamine hydrochloride were purchased from Sigma-Aldrich (Taufkirchen, Germany); N,O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) plus 1% trimethylchlorosilane (TMCS) was purchased from Pierce Chemical Co (Rockford, IL, USA) and references masses purine and HP-0921 (hexakis-(1H,1H,3Htetrafluoropentoxy)-phosphazene) were from Agilent (atmospheric pressure inlet – time of flight (API-TOF) reference mass solution kit). Milli-Qplus 185 was provided by Millipore (Billerica, MA, USA).
3
Bovine Lactoferrin Purification and Labeling
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The bioactive Lf from bovine whey was kindly supplied by the Tatua Cooperative Dairy Company Ltd (New Zealand). Poly-L-Lysine hydrochloride (PLL, Mw 15,000–30,000), BSA lyophilized powder (≥96%), TA (ACS grade), BSA conjugated with fluorescein isothiocyanate (BSA/FITC), bile salts (for microbiology), pancreatin from porcine pancreas (≥100 USP U/mg), pepsin from porcine gastric mucosa (3802 U/mg), mini protease inhibitor cocktail cOmplete™, hydrochloric acid, calcium chloride dehydrate, sodium bicarbonate, sodium chloride, sodium hydroxide, acetonitrile (for HPLC, ≥99.9%) were purchased from Sigma-Aldrich. Anhydrous sodium carbonate was purchased from Alfa Aesar. 0.1% aqueous solution of trifluoroacetic acid (TFA, LC/MS grade, ≥99.99%) was purchased from VWR Chemicals. DQ™ Red BSA was purchased from Molecular Probes Inc, USA. The bovine lactoferrin ELISA kit was purchased from Bethyl Laboratories, Inc., USA. Cyanine 7 N-hydroxysuccinimide ester (Cy7-NHS) was purchased from Lumiprobe Corporation, USA. Cy7-labelled Lf (Cy7-Lf) has been prepared according to a standard protocol provided by Lumiprobe Co and purified by dialysis. All chemicals were used as received without further purification. Deionized (DI) water (specific resistivity higher than 18.2 MΩcm) from Milli-Q plus 185 (Millipore) water purification system was used to make all solutions.
4
RP-HPLC Analysis of BSA Concentration
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The concentration of the sample from the FDC experiment was analysed by RP-HPLC. The diode array detector (DAD) was set at 232 nm. A spectrophotometer (SHIMADZU UV mini 1240) was used to determine the specific wavelength when the light absorbance was at the maximum. A gradient method was adopted with eluent A: 0.1 % TFA in water and B: 0.08 % TFA in acetonitrile, with a mobile phase ratio of A: B, 95:05 to 20:80. The sample size of each injection was 10 μL. The temperature was set to 24 °C. The flow rate was set to 1 ml/min. A complete RP-HPLC run took approximately 20 min with a down time of 2 min between each run. An external standard approach was used for the standardisation for the analysis of the component sample BSA. External standard samples were prepared using pure chemicals of BSA purchased from Sigma-Aldrich and dissolved in deionised water, purified by Millipore Milli-Q Plus 185. Standards of concentration ranging 10–100 μg/ml BSA were prepared and analysed by HPLC to obtain the absorbance.
5
Cadmium Quantum Dots from Sugarcane
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Field samples were collected from different sugarcane varieties grown in the Fusui region, Guangxi province, China. Leaves from varying sugarcane plants were collected and stored at −20 °C until use. All chemicals, including cadmium chloride (CdCl2), mercaptoacetic acid (TGA), tellurium powder (Te), sodium borohydride (NaBH4), 1-(3-dimethyl aminopropyl)-3-ethyl carbodiimide hydrochloride (EDC), sodium citrate, urea, and thiourea, were purchased from Aladdin company (Shanghai, China). All used water was purified by an ultra-pure water purifier (Milli-Q plus 185, Millipore, Billerica, MA, USA).
All fluorometry studies were performed using a Tecan Infinite F200 micro-plate reader (Tecan, Mannedorf, Switzerland). All spectrophotometer analysis was performed using a Shimadzu UV-1800 Ultraviolet-visible spectroscopy spectrophotometer (Shimadzu, Kyoto, Japan). The transmission electron microscopy (TEM) images of the prepared QDs were acquired on a FEI Talos F200s (Hillsboro, OR, USA).
All fluorometry studies were performed using a Tecan Infinite F200 micro-plate reader (Tecan, Mannedorf, Switzerland). All spectrophotometer analysis was performed using a Shimadzu UV-1800 Ultraviolet-visible spectroscopy spectrophotometer (Shimadzu, Kyoto, Japan). The transmission electron microscopy (TEM) images of the prepared QDs were acquired on a FEI Talos F200s (Hillsboro, OR, USA).
6
Quantifying Sucralose in Water Samples
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The Sucralose concentrations were determined in duplicate samples representing controls and nominal concentrations of 5, 50, 500 and 5000 µgL−1; for logistical reasons, only 50% of all treatment samples and controls were analysed (Table S1 in File S1 ), which was considered acceptable given the low between-replicate variability in the analyzed samples. Sucralose (purity 98%) and Sucralose-d6 (surrogate standard, isotopic purity >98%) were purchased from Toronto Research Chemicals Inc. (North York, Canada). Ammonium hydroxide 25% (puriss) was purchased from Fluka (Buchs, Switzerland). Methanol (LichroSolve) was purchased from Merck (Darmstadt, Germany). Water was of milli-Q grade from a milli-Q ultrapure water system, MilliQ PLUS 185 from Millipore (Stockholm, Sweden). Sucralose was extracted from the water samples by solid phase extraction [2] . Briefly, surrogate standard was added to 5 mL of water sample and Sucralose was enriched on an Oasis HLB 60 mg 3 cc cartridge (Waters corp.). After elution with 2 mL methanol and change of solvent, the concentrations of Sucralose were determined by electrospray LC/MS/MS.
7
Oligonucleotide Characterization Protocol
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Hombikat UV100 (>99% anatase, 5 nm particle size, supplied by Sachtleben Chemie); luminol, hemin, DMSO (99.9%, anhydrous (max. 0.005% H2O)) and sodium hydroxide (NaOH) were acquired from Sigma Aldrich. Millipore water (Milli-Q Plus 185) was used for preparation of aqueous solutions and sample washing. RNAse-free water was purchased from Fisher Scientific, Inc. (Pittsburgh, PA) and used for all buffers and for the stock solutions of oligonucleotides. The oligonucleotides were obtained from Integrated DNA Technologies, Inc. (Coralville, IA). The oligonucleotides were dissolved in water and stored at −20 °C until needed. Stock concentrations of oligonucleotides were calculated by measuring the absorption of the solutions at 260 nm by using a Perkin-Elmer Lambda 35 UV/Vis spectrometer (San Jose, CA). Extinction coefficients of oligonucleotides were calculated by using OligoAnalyzer 3.1 software (Integrated DNA Technologies, Inc.). All experiments were carried out in the buffer, containing 50 mM HEPES pH 6.6., 50 mM MgCl2, 20 mM K+, 120 mM NaCl, 1% DMSO v/v, 0.03% w/v Triton X 100, 38% w/v acrylamide, 2% w/v bisacrylamide, 127 mM acetylacetone.
8
DOTA-Dendrimer-Hyaluronic Acid Nanoplatform
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Ethylenediamine core amine-terminated G5.NH2 poly(amidoamine) dendrimers with a polydispersity index (PDI) less than 1.08 were purchased from Dendritech (Midland, MI). DOTA-NHS was purchased from CheMatech (Dijon, France). HA with molecular weight of 6 k Da, sodium hydroxide, EDC, and N-hydroxysuccinimide (NHS) were purchased from J&K Chemical Ltd. (Shanghai, China). HCCLM3 cells (an HCC cell line) were obtained from the Liver Cancer Institute, Zhongshan Hospital, Fudan University (Shanghai, China). Fetal bovine serum (FBS), streptomycin, penicillin, and Dulbecco’s modified Eagle medium (DMEM) were obtained from Hangzhou Jinuo Biomedical Technology (Hangzhou, China). Triethylamine, acetic anhydride, HAuCl4·4H2O, and all other chemicals and solvents were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). The water used in all experiments was purified by a Milli-Q Plus 185 water purification system (Millipore, Bedford, MA) with a resistivity greater than 18 MΩ cm. Regenerated cellulose dialysis membranes (MWCO = 14,000 or 1,000) were acquired from Thermo Fisher Scientific (Pittsburgh, PA).
9
Synthesis of Calcium Acetate Hydrogels
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All chemical reagents are commercially available. Calcium acetate monohydrate (Ca(OAc)2H2O) and poly(acrylic) acid sodium salt (average MW = 8000 Daltons) were purchased from Sigma Aldrich (USA). The water used in all experiments was prepared in a three-stage Millipore MilliQ plus 185 purification system, and had a resistivity of 18.2 mΩ cm.
10
Synthesis and Characterization of Silica-Based Photocatalysts
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Sodium hydroxide (NaOH)
(99% purity) and sodium sulfide (Na2S) were used for alkaline
treatment; sodium chlorite (NaClO2), glacial acetic acid,
and sodium hydroxide (buffer solution) were used as bleaching agents,
while sulfuric acid (95–98% purity) was used for acid hydrolysis.
Dialysis membranes (cellulose membranes with an average flat width
of 10 mm and molecular-weight cutoff: 6000–8000 Da) were used
as received, tetraethoxysilane (TEOS, >95%) and ethanol (EtOH,
>99.7%)
were used during the silica preparation, MB dye (C16H18ClN3S, >95%) was used as a model pollutant
in
the photocatalysis study. Pure water from a Millipore Milli-Q Plus 185 purification system (resistivity 18.2 MU cm)
was used for all experiments. All the chemicals purchased from VWR
Chemicals and Sigma-Aldrich were of reagent grade and were used as
received without any further purification.
(99% purity) and sodium sulfide (Na2S) were used for alkaline
treatment; sodium chlorite (NaClO2), glacial acetic acid,
and sodium hydroxide (buffer solution) were used as bleaching agents,
while sulfuric acid (95–98% purity) was used for acid hydrolysis.
Dialysis membranes (cellulose membranes with an average flat width
of 10 mm and molecular-weight cutoff: 6000–8000 Da) were used
as received, tetraethoxysilane (TEOS, >95%) and ethanol (EtOH,
>99.7%)
were used during the silica preparation, MB dye (C16H18ClN3S, >95%) was used as a model pollutant
in
the photocatalysis study. Pure water from a Millipore Milli-Q Plus 185 purification system (resistivity 18.2 MU cm)
was used for all experiments. All the chemicals purchased from VWR
Chemicals and Sigma-Aldrich were of reagent grade and were used as
received without any further purification.
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