Squalene oil was purchased from Alfa Aesar (Seoul, Korea), Span 80 from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) and Cremophor ELP from BASF (Ludwigshafen, Germany). Two different grades of carbomers, C-971P NF and C-940, Montanide ISATM 201 and an antigen solution incorporating a PCV type 2 antigen and inactivated Mycoplasma hyopneumoniae (J101 strain, phosphatase (−), arginine hydrolysis (−), urease (−)) antigen were supplied from Komipharm Int’l Co., Ltd. (Siheung, Korea). DNA extraction kit and PCR premix were purchased from iNtron Biotechnology (Seongnam, Korea). Rabbit anti-Mouse IgG2a secondary antibody and horseradish peroxidase (HRP) was purchased from Thermofisher (Waltham, MA, USA), mouse IgG1 antibody (also known as goat anti-Mouse IgG1, HRP) from GeneTex (Hsinchu City, Taiwan) and goat anti-Mouse IgG (H+L) secondary antibody was purchased from Thermofisher (Waltham, MA, USA). The Mycoplasma hyopneumoniae antibody test kit was purchased from IDEXX (Westbrook, ME, USA). Other used reagents were of analytical grade.
Span 80
Manufactured by Tokyo Chemical Industry
Sourced in Japan
Span 80 is a nonionic surfactant. It is a viscous, colorless liquid that is soluble in organic solvents. Span 80 acts as an emulsifier, dispersant, and wetting agent.
Automatically generated - may contain errors
Lab products found in correlation
Tween 80, by Tokyo Chemical Industry (3 mentions)
α tocopherol, by Merck Group (2 mentions)
Tricaprylin, by Merck Group (2 mentions)
Goat anti mouse igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions)
Cremophor elp, by BASF (1 mentions)
Pcr premix, by iNtRON Biotechnology (1 mentions)
Dna extraction kit, by iNtRON Biotechnology (1 mentions)
Ammonium persulfate, by Fujifilm (1 mentions)
Chondroitin sulfate c sodium salt, by Fujifilm (1 mentions)
N n dimethylacrylamide, by Fujifilm (1 mentions)
N n dimethylaminoethyl methacrylate, by Fujifilm (1 mentions)
Milli q water, by Merck Group (1 mentions)
α hemolysin, by Merck Group (1 mentions)
Staphylococcus aureus, by Merck Group (1 mentions)
Rho pe, by Avanti Polar Lipids (1 mentions)
Su 8 3025, by MicroChem (1 mentions)
Tween 20, by Merck Group (1 mentions)
Phosal 50 pg, by Lipoid (1 mentions)
Nerolidol, by Thermo Fisher Scientific (1 mentions)
Luteolin, by Tokyo Chemical Industry (1 mentions)
7 protocols using span 80
1
Swine Mycoplasma Vaccine Development
2
Synthesis of DMAA-DMAEMA Copolymers
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Monomers, N,N-dimethylacrylamide (DMAA) (049-19185), N,N-dimethylaminoethyl methacrylate (DMAEMA) (044-16276), and the cross-linker N,N′-methylenebisacrylamide (M0506) were obtained from Wako Pure Chemical Industries Ltd. (Osaka, Japan) and Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), respectively. The stabilizers Span 80 and Tween 80 used during polymerization were purchased from Tokyo Chemical Industry Co., Ltd. and Sigma-Aldrich Co. (St. Louis, USA), respectively. The initiator ammonium persulfate (018-03282) was obtained from Wako Pure Chemical Industries, Ltd. The column (I.D. 5.0 mm, length 10 cm) used for gel packing was obtained from Bio-Rad (Hercules, USA). Sodium glucuronic acid monohydrate and chondroitin sulfate C sodium salt used for adsorption were purchased from Wako Pure Chemical Industries, Ltd. Other chemicals were of analytical grade or higher.
3
Droplet-Interface Bilayer Microfluidics
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A zwitterionic lipid, PC (Avanti Polar Lipids, Alabaster, AL, USA), and a nonionic surfactant, sorbitan monooleate (Span80, Tokyo Chemical Industry Co., Tokyo, Japan), were dissolved in hexadecane (purity > 98%; Tokyo Chemical Industry Co., Tokyo, Japan) and used as the continuous oil phase. Milli-Q water (H20MB0124, Merck KGaA) was used as the aqueous phase. To visualize a lipid membrane covering a droplet, a fluorescent-labeled lipid, 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt; Rho-PE, Avanti Polar Lipids) was used. Calcein (Sigma-AldrichSt. Louis, MO, USA), a water-soluble fluorescein, was used to analyze the function of α-hemolysin isolated from Staphylococcus aureus (Sigma-Aldrich) reconstituted in a DIB. The microfluidic device was developed using a photoresist (SU-8 3025, MicroChem Corp., Westborough, MA, USA) and a polydimethylsiloxane (PDMS) package (Sylgard 184, Dow Corning Toray Corp., Tokyo, Japan).
4
Luteolin-Enriched Topical Formulation
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Luteolin [2-(3,4-dihydroxyphenyl)-5,7-dihydroxychromen-4-one, CAS#491-70-3)] with a purity >98%, Tween® 80 (Polyoxyethylene 20 sorbitan monooleate, CAS#9005-65-6), and Span® 80 [(Z)-Sorbitan mono-9-octadecenoate, CAS#1338-43-8] were purchased from Tokyo Chemical Industry (Tokyo, Japan). Castor oil, jojoba oil, and shea butter were obtained from Magie Fairy (Taichung city, Taiwan). PHOSAL® 50 PG (Phosphatidylcholine in propylene glycol, content ≥ 50.0%) was obtained from LIPOID® GmbH (Ludwigshafen, Germany). Kolliphor® EL (Polyethoxylated castor oil, CAS#61791-12-6) was supplied by BASF (Ludwigshafen, Germany) and Tween® 20 (Polyoxyethylene 20 sorbitan monolaurate, CAS#9005-64-5) was from Sigma-Aldrich Co. (St. Louis, MO, USA). 1,8-cineole (1,3,3-trimethyl-2-oxabicyclo [2.2.2]octane, CAS# 470-82-6) and nerolidol [(6E)-3,7,11-trimethyldodeca-1,6,10-trien-3-ol, CAS# 7212-44-4)] were purchased from Alfa Aesar (Haverhill, MA, USA). The MultiScreen® Permeability Filter Plate, 0.4 µm, non-sterile (The skin PAMPA kit) was from Merck (Darmstadt, Germany). All solvents used in this study were of analytical grade.
5
Quantitative Analysis of Bioactive Compounds
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AG (purity: ≥98.0%), sorbitan monooleate (Span® 80), polysorbate 80 (Tween® 80), polysorbate 20 (Tween® 20), dimethyl sulfoxide (DMSO), and fisetin as the internal standard (ISTD; purity: >96.0%) were obtained from Tokyo Chemical Industry (Tokyo, Japan). Polyoxyl 35 castor oil (Cremophor EL®; BASF Corporation, Ludwigshafen, Germany), α-tocopherol, tricaprylin, and INDO were supplied by Sigma-Aldrich Co. (St Louis, MO, USA). (+)-Limonene was purchased from Acros Organics (Morris, NJ, USA). Acetonitrile for high performance liquid chromatography (HPLC) and methyl tert-butyl ether (purity: ≥99.8%) were obtained from Tedia Company, Inc. (Fairfield, OH, USA).
6
Fabrication of Polyvinylidene Fluoride Membrane
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Non-woven polypropylene support (PP, Novatexx 2471) was purchased from Freudenberg-Filter (Weinheim, Germany). Polyvinylidene fluoride (PVDF, KYNAR HSV900) was provided by Arkema (Colombes, France) which was used as the membrane polymer. N, N-Dimethylacetamide (DMAc) (Purity ≥ 99.9%, Sigma-Aldrich, St. Louis, MO, USA) was utilized as the organic solvent for the preparation of the PVDF dope. The dopamine hydrochloride (DA, 98%), Oil red O, and Tris (hydroxymethyl) aminomethane hydrochloride (Tris-HCl) were supplied by Aladdin Reagent Co., Ltd. (Shanghai, China). Sodium dodecyl sulfate (SDS) was provided by Sigma-Aldrich. Span-80 was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Deionized water was produced from a Milli-Q® water purification system (Merck Millipore, Burlington, MA, USA) and used in all the experiments.
7
Formulation of Lipid-Based Drug Delivery Systems
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RES (purity > 98%), Span 80, Tween 80, and Tween 20 were obtained from Tokyo Chemical Industry (Tokyo, Japan). Capryol 90 (propylene glycol monocaprylate) was purchased from Gattefosse (Saint-Priest Cedex, France). Tricaprylin (glycerol trioctanoate), Cremophor EL (polyoxyl 35 hydrogenated castor oil) and α-tocopherol were supplied by Sigma-Aldrich (St. Louis, MO, USA). Triacetin (glyceryl triacetate) was obtained from Alfa Aesar (Ward Hill, MA, USA). Span 65 was purchased from Merck (Darmstadt, Germany). Acetonitrile for HPLC were obtained from Tedia (Fairfield, OH, USA).
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