T sod a001 1

T-SOD (A001-1) and MDA (A003-1) kits were purchased from Jiancheng Bioengineering Institute (Nanjing, China). Briefly, kidney tissues were prepared into 10% homogenates which were centrifuged at 3,000 rpm for 10 min. The supernatants were collected to measure levels of T-SOD and MDA according to the instructions.

Eight rats in each group were collected in a metabolic cage for urine collection, then blood was collected through the abdominal aorta, and supernatant was obtained by centrifugation at 3000 rpm for 15 min. The right kidney of these eight rats was extracted to obtain kidney tissue homogenate according to the kit instructions. The levels of creatinine (Cr, C011‐2‐1, Nanjing Jiancheng), urea nitrogen (BUN, C013‐2‐1, Nanjing Jiancheng), urinary protein (UP, C035‐2‐1, Nanjing Jiancheng) in the serum and urine, superoxide dismutase (T‐SOD, A001‐1, Nanjing Jiancheng), glutathione peroxidase (GSH‐Px, A005, Nanjing Jiancheng) and micro malondialdehyde (MDA, A003‐1, Nanjing Jiancheng) in the kidney tissue were measured.