Universal atr spectrometer

Manufactured by PerkinElmer

Sourced in United States

The Universal ATR spectrometer is a versatile laboratory instrument designed for infrared (IR) spectroscopy analysis. It employs the attenuated total reflection (ATR) technique to collect infrared spectra of solid, liquid, and semi-solid samples without specialized sample preparation. The core function of the Universal ATR spectrometer is to provide users with a convenient and efficient way to obtain high-quality IR data for a wide range of materials.

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Lab products found in correlation

Fe sem, by Zeiss (3 mentions) Multspec 1501, by Shimadzu (3 mentions) Avance 3, by Bruker (2 mentions) Uv 3600 spectrometer, by Shimadzu (1 mentions) Avance 3 400 mhz, by Brucker (1 mentions) Cary uv vis spectrophotometer, by Agilent Technologies (1 mentions) Silica gel 60, by Merck Group (1 mentions) Microtome, by Leica (1 mentions) Cm 120 model, by Philips (1 mentions) Merck silica gel 60, by Merck Group (1 mentions)

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Universal ATR Spectrometers

10 protocols using universal atr spectrometer

Characterization of HAART-AgNPs Conjugates

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The characterisation of AgNPs and HAART-AgNPs was previously done (Lawal et al., 2021 (link)). Briefly, Fourier Transform Infrared (FTIR) spectroscopy (Perkin-Elmer Universal ATR spectrometer, USA) was used to identify the various functional groups in the HAART + AgNPs conjugates. The ultraviolet-visible (UV-Vis) spectroscopy (Shimadzu MultSpec-1501, Shimadzu Corporation, Tokyo, Japan) was used to confirm the absorption of the conjugated HAART-AgNPs. The size and morphology of the nanoparticles were examined by a high-resolution transmission electron microscope (HR-TEM, JEOL 2100, Japan) operated at a voltage of 200 kV.
The field emission scanning electron microscope (FESEM, Carl Zeiss, Germany) operated at a voltage of 5 kV with energy dispersive X-ray (EDX, Aztec Analysis Software, England) was used to determine the elemental components.

Lawal S.K., Olojede S.O., Dare A., Faborode O.S., Sulaiman S.O., Naidu E.C., Rennie C.O, & Azu O.O. (2022). Highly active antiretroviral therapy-silver nanoparticle conjugate interacts with neuronal and glial cells and alleviates anxiety-like behaviour in streptozotocin-induced diabetic rats. IBRO Neuroscience Reports, 13, 57-68.

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Comprehensive Characterization of Novel Compounds

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NMR spectra (1D and 2D) were recorded in deuterated MeOH (CD3OD) and deuterated chloroform (CDCl3) at room temperature using a Brucker Avance^III 400 MHz and 600 MHz spectrometer with tetramethylsilane (TMS) as an internal standard. IR spectra were recorded using a Perkin Elmer Universal ATR Spectrometer. UV spectra were recorded using the UV-Vis-NIR Shimadzu UV-3600 spectrometer with MeOH as a solvent. GC-MS data were recorded on an automated GC-MS (split-less mode) equipped with a DB-5SIL MS fused silica capillary column (30 m x 0.25 mm i.d., 0.25 μm film thickness). Helium (0.70 mL min^-1) was used as a carrier gas and acetonitrile (ACN) was used to dissolve the sample. 1 μL of each sample solution was injected into the GC-MS. The injector was kept at 250 °C whilst the transfer line was at 280 °C. The column temperature was held at 60 °C for 2 min, and then ramped to 280 °C at 20 °C min^-1 where it was held for 10 min. The MS was operated in the EI mode at 70 eV. Melting points (uncorrected) were recorded on an Ernst Leitz Wetzlar micro-hot stage melting point apparatus. All chemicals used were supplied by Merck and Sigma Chemical Companies and were of analytical-reagent grade. All spectral data obtained were compared with those reported in literature for identification of known compounds.

Hamza M.F., Shaik S, & Moodley R. (2016). PHYTOCHEMICAL, ELEMENTAL AND BIOTECHNOLOGICAL STUDY OF CRYPTOCARYA LATIFOLIA. African Journal of Traditional, Complementary, and Alternative Medicines, 13(4), 74-80.

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Characterization of HAART-AgNPs Conjugates

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The shape, size, morphology, functional group and elemental composition of the conjugates were examined. The absorption of the conjugates was measured by the Ultraviolet-visible (UV-Vis) spectroscopy (Shimadzu MultSpec-1501, Shimadzu Corporation, Tokyo, Japan). The size and morphology of the nanoparticles were examined by a high-resolution transmission electron microscope (HR-TEM, JEOL 2100, Japan) operated at a voltage of 200 kV, and field emission scanning electron microscope (FESEM, Carl Zeiss, Germany) operated at a voltage of 5 kV with energy dispersive x-ray (EDX, Aztec Analysis Software, England). The high-resolution transmission electron microscope (HTEM) and the field emission scanning electron microscope (SEM) confirmed the shape and the size of the AgNPs. Also, the Fourier transform infrared (FTIR) spectroscopy (PerkinElmer Universal ATR spectrometer, USA) confirmed the conjugation of HAART to AgNPs while Energy-dispersive X-ray spectroscopy (EDX) confirmed the presence of elemental constituents of the HAART-AgNPs (Lawal et al., 2021 (link)).

Olojede S.O., Lawal S.K., Dare A., Moodley R., Rennie C.O., Naidu E.C, & Azu O.O. (2021). Highly active antiretroviral therapy conjugated silver nanoparticle ameliorates testicular injury in type-2 diabetic rats. Heliyon, 7(12), e08580.

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Characterization of Nanoconjugates for HAART

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The formulated nanoconjugates (AgNPs and HAART-AgNPs) were confirmed by ultraviolet-visible (UV-Vis) spectroscopy (Shimadzu MultSpec-1501, Shimadzu Corporation, Tokyo, Japan) and Fourier transform infrared (FTIR) spectroscopy (Perkin-Elmer Universal ATR spectrometer, USA). The morphology and size of the nanoparticles were evaluated by a high-resolution transmission electron microscope (HR-TEM, JEOL 2100, Japan) operated at a voltage of 200 Kv. Field emission scanning electron microscope (FESEM, Carl Zeiss, Germany) performed at a voltage of 5 keV with energy dispersive X-ray (EDX, Aztec Analysis Software, England).

Lawal S.K., Olojede S.O., Dare A., Faborode O.S., Naidu E.C., Rennie C.O, & Azu O.O. (2021). Silver Nanoparticles Conjugate Attenuates Highly Active Antiretroviral Therapy-Induced Hippocampal Nissl Substance and Cognitive Deficits in Diabetic Rats. Journal of Diabetes Research, 2021, 2118538.

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Infrared Analysis of TiO2–HAP and HAP

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To analyze the chemical components of samples, the infrared spectra were measured using a Perkin Elmer Universal ATR spectrometer to identify the functional group constituents of TiO₂–HAP and HAP.

Yin L., Xu X., Chu C., Lin P., Huang H., Luo B, & Yang C. (2022). In-vitro characterization and evaluation of mesoporous titanium dioxide composite hydroxyapatite and its effectiveness in occluding dentine tubules. BMC Oral Health, 22, 43.

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Spectroscopic Characterization of Organic Compounds

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Solvents used for the extraction and purification were reagent grade and distilled prior to being used. 1 H, 13 C and 2D NMR spectra were recorded on Bruker Avance III 400 and 600 MHz spectrometers. The spectra were referenced according to the deuteriochloroform signals at δ H 7.24 and δ C 77.0 and dimethyl sulfoxide δ H 2.50 and δc 39.51 (for 1 H NMR and 13 C NMR spectra, respectively). The EIMS was measured on a ThermoFinnigan trace 132 GC, coupled with a Polaris Q mass spectrometer. IR spectra were recorded using a Perkin Elmer Universal ATR spectrometer. UV spectra were obtained on a Varian Cary UV-VIS Spectrophotometer. The melting points were determined on an Ernst Leitz Wetziar micro-hot stage melting point apparatus. Merck silica gel 60 (0.040-0.063 mm) was used for column chromatography and Merck 20 cm x 20 cm silica gel 60 F 254 aluminium sheets were used for TLC. The TLC plates were analyzed under UV (254 and 366 nm) before being sprayed and developed with anisaldehyde: concentrated sulfuric acid: methanol spray reagent.

Aliyu A.B., Koorbanally N.A., Moodley B, & Chenia H.Y. (2021). Sesquiterpene lactones from Polydora serratuloides and their quorum sensing inhibitory activity. Natural product research, 35(22).

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Multi-Technique Characterization of EB-TiO2

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Fourier Transform Infrared Spectroscopy analysis. The infrared spectra were measured using a Perkin Elmer Universal ATR spectrometer to identify the functional group constituents of EB-TiO 2 . A very small amount of sample was placed in the sample holder. An initial background check was performed before scanning in the range of 400-4500 cm -1 .
X-Ray Diffraction analysis. The crystallinity of the modified Eb-TiO 2 was assessed using X-Ray Diffraction (XRD). The XRD diffractometer (PANalytical-Empyrean instrument; Co radiation 1.54056 A o ) was calibrated with a voltage of 40 kV, current of 40 mA, and time of 1 s and analyzed between 0° and 90 o (2 theta).
Transmission Electron Microscopy analysis. Transmission Electron Microscopy (TEM) was used to observe the particle size, shape, and distribution of EB-TiO 2 . Some amounts of the samples were dispersed in 5 ml ethanol and sonicated at 10 kV for 20 min. Then, thin cross-sections of cryo-microtomed specimens were prepared using a Leica microtome (South Africa) and placed on carbon copper grids. Analysis was conducted using a transmission electron microscope (TEM-Philips CM 120 model) at 120 kV.

Onwubu S.C., Mdluli P.S, & Singh S. (2019). Evaluating the buffering and acid-resistant properties of eggshell-titanium dioxide composite against erosive acids. Journal of applied biomaterials & functional materials, 17(1).

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FTIR Spectroscopy of EPAM

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The infrared spectra for Fourier transform infrared (FTIR) spectroscopy were measured using a Perkin Elmer Universal ATR spectrometer to identify the structure and the functional group constituents of EPAM.

Onwubu S.C., Vahed A., Singh S, & Kanny K.M. (2017). Physicochemical characterization of a dental eggshell powder abrasive material. Journal of applied biomaterials & functional materials, 15(4).

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Spectroscopic Characterization of Compounds

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Ultraviolet (UV) spectra were obtained on a Hewlett Packard UV-3600 spectrophotometer. Infrared (IR) spectra were recorded using a Perkin-Elmer Universal ATR spectrometer. The 1 H, 13 (link) C and two-dimensional nuclear magnetic resonance (2D-NMR) spectra were recorded in deuterated CDCl 3 and DMSO (Merck, Darmstadt, Germany) using a 400-MHz spectrometer (Avance III, Bruker, Rheinstetten, Germany). High-resolution mass spectra were recorded using a time-of-flight mass spectrometer (LCT Premier TOF-MS, Waters Micro-mass, Milford, MA, USA). Column chromatography was performed with Merck silica gel 60 (0.040-0.063 mm) and Sephadex LH-20 (25-100 µm bead size, Sigma-Aldrich, Germany). Thin-layer chromatography was performed on Merck 20 × 20 cm silica gel 60, F 254 aluminium sheets. The spots were analysed under UV (254 nm and 366 nm), visualised using 10% H 2 SO 4 in MeOH, followed by heating. Solvents (analytical grade) and other chemicals used were supplied by either Merck (Darmstadt, Germany) or Sigma (St. Louis, MO, USA) chemical companies.

Ogunlaja O.O., Moodley R., Baijnath H., & Jonnalagadda S.B. (2022). Antioxidant activity of the bioactive compounds from the edible fruits and leaves of Ficus sur Forssk. (Moraceae). South African Journal of Science, 118(3/4).

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Structural Characterization of Eggshell-Derived Biomaterials

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Fourier transform infrared spectroscopy. The Fourier transform infrared (FTIR) spectra were measured using a Perkin Elmer Universal ATR spectrometer to identify the functional group constituents of eggshell powder, the calcined eggshell powder, and EnHAp. A very small amount of each sample was placed in the sample holder. An initial background check was performed before scanning in the range 400-4500 cm -1 at a resolution of 4 cm -1 .
X-ray diffraction. X-ray diffraction (XRD) analysis was performed to observe the possible changes in crystallinity between the eggshell powder, calcined eggshell powder, and EnHAp. The XRD patterns were recorded using a diffractometer (PANalytical-Empyrean instrument; Co radiation 1.54056 Å) and analyzed between 0 and 90° (2θ). The voltage, current, and pass time used were 40 kV, 40 mA, and 1 s, respectively.
Field emission scanning electron microscopy. An SEM (field emission, Carl Zeiss) operating in controlled atmospheric conditions at 20 kV was used to examine the surface morphology of the samples. Prior to SEM observation, the surface was coated with a thin, electric conductive gold film to prevent a build-up of electrostatic charge.

Onwubu S.C., Mhlungu S, & Mdluli P.S. (2019). In vitro evaluation of nanohydroxyapatite synthesized from eggshell waste in occluding dentin tubules. Journal of applied biomaterials & functional materials, 17(2).

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