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Egfr chr7 probe egfr chr07 20 orgr

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The EGFR-Chr7 probe (EGFR-CHR07–20-ORGR) is a DNA probe designed to detect the epidermal growth factor receptor (EGFR) gene located on chromosome 7. This probe can be used in fluorescence in situ hybridization (FISH) techniques to visualize and analyze the EGFR gene.

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Lab products found in correlation

Vectashield mounting medium with dapi, by Vector Laboratories (2 mentions) Hybridization buffer, by Empire Genomics (2 mentions) Sted 3x dls confocal, by Leica (2 mentions)

2 protocols using egfr chr7 probe egfr chr07 20 orgr

1

EGFR FISH Tissue Touch Prep Protocol

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Tissue slides were prepared by tumor touch prep method46 (link). Positively charged glass slides were pressed against the surface of thawed frozen tissues. The slides were then immediately fixed by cold Carnoy’s fixative (3:1 methanol:glacial acetic acid, v/v) for 30 minutes and then air-dried. Slides were denatured in hybridization buffer (Empire Genomics) mixed with EGFR-Chr7 probe (EGFR-CHR07–20-ORGR, Empire Genomics) at 75°C for 5 minutes and then incubated at 37°C overnight. The post-hybridization wash was with 0.4x SSC at 75°C for 3 minutes followed by a second wash with 2x SSC/0.05% Tween20 for 1 minute. The slides were then briefly rinsed by water and air-dried. The VECTASHIELD mounting medium with DAPI (Vector Laboratories) was applied and the coverslip was mounted onto a glass slide. Tissue images were scanned under Leica STED 3X/DLS Confocal with 100x magnification.
Johnson K.C., Anderson K.J., Courtois E.T., Gujar A.D., Barthel F.P., Varn F.S., Luo D., Seignon M., Yi E., Kim H., Estecio M.R., Zhao D., Tang M., Navin N.E., Maurya R., Ngan C.Y., Verburg N., de Witt Hamer P.C., Bulsara K., Samuels M.L., Das S., Robson P, & Verhaak R.G. (2021). Single-cell multimodal glioma analyses identify epigenetic regulators of cellular plasticity and environmental stress response. Nature genetics, 53(10), 1456-1468.
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2

EGFR FISH Tissue Touch Prep Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue slides were prepared by tumor touch prep method46 (link). Positively charged glass slides were pressed against the surface of thawed frozen tissues. The slides were then immediately fixed by cold Carnoy’s fixative (3:1 methanol:glacial acetic acid, v/v) for 30 minutes and then air-dried. Slides were denatured in hybridization buffer (Empire Genomics) mixed with EGFR-Chr7 probe (EGFR-CHR07–20-ORGR, Empire Genomics) at 75°C for 5 minutes and then incubated at 37°C overnight. The post-hybridization wash was with 0.4x SSC at 75°C for 3 minutes followed by a second wash with 2x SSC/0.05% Tween20 for 1 minute. The slides were then briefly rinsed by water and air-dried. The VECTASHIELD mounting medium with DAPI (Vector Laboratories) was applied and the coverslip was mounted onto a glass slide. Tissue images were scanned under Leica STED 3X/DLS Confocal with 100x magnification.
Johnson K.C., Anderson K.J., Courtois E.T., Gujar A.D., Barthel F.P., Varn F.S., Luo D., Seignon M., Yi E., Kim H., Estecio M.R., Zhao D., Tang M., Navin N.E., Maurya R., Ngan C.Y., Verburg N., de Witt Hamer P.C., Bulsara K., Samuels M.L., Das S., Robson P, & Verhaak R.G. (2021). Single-cell multimodal glioma analyses identify epigenetic regulators of cellular plasticity and environmental stress response. Nature genetics, 53(10), 1456-1468.
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