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Observer z1 inverted microscope

Manufactured by Yokogawa
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Sourced in Japan

The Observer.Z1 is an inverted microscope designed for a wide range of research and analysis applications. Its core function is to provide high-quality imaging and observation capabilities for specimens located below the objective lens.

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Lab products found in correlation

Csu x1 spinning disk unit, by Yokogawa (2 mentions) Nis element, by Nikon (1 mentions)

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Observer Z1 microscope (3 citations)

5 protocols using observer z1 inverted microscope

1

Multimodal Imaging of Live Specimens

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Imaging was performed on a Zeiss Observer.Z1 inverted microscope equipped with a Yokogawa CSU-X1 spinning disk unit; 405 nm/50 mW, 445nm/40 mW, 488 nm/40 mW, 515 nm/50 mW, 561 nm/50 mW and 640 nm/50 mW lasers; an EC Plan-Neofluar 40x (1.30 NA) oil immersion objective lens; an mSAC unit for correction of spherical ablation (Intelligent Imaging Innovations); a Vector live specimen scanner (Intelligent Imaging Innovations) for positioning of the photo-uncaging laser; a Photometrics PVCAM (Dynamic) EMCCD camera with 16-bit dynamic range and an mSwitcher ms optical switching unit (Intelligent Imaging Innovations) that enabled simultaneous use of the CSU-X 1 and Vector units. Instrumentation was controlled by SlideBook 6.0 software (Intelligent Imaging Innovations). Data acquisition and analysis were performed with Slidebook 6.0 software and ImageJ software.
Wild A.R., Sinnen B.L., Dittmer P.J., Kennedy M.J., Sather W.A, & Dell’Acqua M.L. (2019). Synapse-to-Nucleus Communication through NFAT Is Mediated by L-type Ca2+ Channel Ca2+ Spike Propagation to the Soma. Cell reports, 26(13), 3537-3550.e4.
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2

Multimodal Imaging of Live Specimens

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Imaging was performed on a Zeiss Observer.Z1 inverted microscope equipped with a Yokogawa CSU-X1 spinning disk unit; 405 nm/50 mW, 445nm/40 mW, 488 nm/40 mW, 515 nm/50 mW, 561 nm/50 mW and 640 nm/50 mW lasers; an EC Plan-Neofluar 40x (1.30 NA) oil immersion objective lens; an mSAC unit for correction of spherical ablation (Intelligent Imaging Innovations); a Vector live specimen scanner (Intelligent Imaging Innovations) for positioning of the photo-uncaging laser; a Photometrics PVCAM (Dynamic) EMCCD camera with 16-bit dynamic range and an mSwitcher ms optical switching unit (Intelligent Imaging Innovations) that enabled simultaneous use of the CSU-X 1 and Vector units. Instrumentation was controlled by SlideBook 6.0 software (Intelligent Imaging Innovations). Data acquisition and analysis were performed with Slidebook 6.0 software and ImageJ software.
Wild A.R., Sinnen B.L., Dittmer P.J., Kennedy M.J., Sather W.A, & Dell’Acqua M.L. (2019). Synapse-to-Nucleus Communication through NFAT Is Mediated by L-type Ca2+ Channel Ca2+ Spike Propagation to the Soma. Cell reports, 26(13), 3537-3550.e4.
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3

Imaging Nuclear NFAT Dynamics

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Hippocampal neurons expressing GFP-NFATc3 were imaged pre-stimulation and 10 minutes post-iLTP induction. Live imaging was performed on a Zeiss Observer.Z1 inverted microscope equipped with a Yokogawa CSU-X1 spinning disk unit; an EC Plan- Neofluar 40x (1.30 NA) oil immersion objective lens; a Photometrics PVCAM (Dynamic) EMCCD camera with 16-bit dynamic range; and SlideBook 6.0 software. Images were acquired using 2 μm Z stacks of xy planes at 0.2 mm intervals. Nuclear GFP-NFATc3 levels were normalized to cytoplasmic levels in ImageJ. Five independent experiments were performed, and statistical significance was determined using paired t tests.
Rajgor D., Purkey A.M., Sanderson J.L., Welle T.M., Garcia J.D., Dell’Acqua M.L, & Smith K.R. (2020). Local miRNA-Dependent Translational Control of GABAAR Synthesis during Inhibitory Long-Term Potentiation. Cell reports, 31(12), 107785.
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4

Imaging Nuclear NFAT Dynamics

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Hippocampal neurons expressing GFP-NFATc3 were imaged pre-stimulation and 10 minutes post-iLTP induction. Live imaging was performed on a Zeiss Observer.Z1 inverted microscope equipped with a Yokogawa CSU-X1 spinning disk unit; an EC Plan- Neofluar 40x (1.30 NA) oil immersion objective lens; a Photometrics PVCAM (Dynamic) EMCCD camera with 16-bit dynamic range; and SlideBook 6.0 software. Images were acquired using 2 μm Z stacks of xy planes at 0.2 mm intervals. Nuclear GFP-NFATc3 levels were normalized to cytoplasmic levels in ImageJ. Five independent experiments were performed, and statistical significance was determined using paired t tests.
Rajgor D., Purkey A.M., Sanderson J.L., Welle T.M., Garcia J.D., Dell’Acqua M.L, & Smith K.R. (2020). Local miRNA-Dependent Translational Control of GABAAR Synthesis during Inhibitory Long-Term Potentiation. Cell reports, 31(12), 107785.
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5

Automated Mitochondrial Morphology Analysis

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z-stacks of mitochondria with 0.2-µm increments were imaged with a Zeiss Observer Z.1 inverted microscope equipped with a Yokogawa CSU-X1 Nipkow spinning disk system, a piezoelectric z-axes motorized stage (CRWG3-200; Nippon Thompson Co., Ltd., Tokyo, Japan), and a CoolSNAP HQ2 CCD Camera (Photometrics). The image stacks were deconvoluted using blind deconvolution (NIS-Elements, Nikon, Austria). Morphology parameters were measured automatically with a custom made ImageJ macro using the following procedure. An additional background subtraction using the rolling ball method was used to further increase contrast for later analysis. Both a global auto Otsu threshold using the stack histogram as well as a local auto Otsu threshold (radium of 640 nm) using the single slice histogram were applied to the stack and merged. The ImageJ plugin 3D manager was used to segment the binarized mitochondria. With the plugin 3D Geometrical Measure the mitochondrial volume and surface were determined. The Plugin 3D Ellipsoid Fitting generated an ellipsoid fit of the mitochondria to measure elongation and flatness parameters.
Gottschalk B., Klec C., Leitinger G., Bernhart E., Rost R., Bischof H., Madreiter-Sokolowski C.T., Radulović S., Eroglu E., Sattler W., Waldeck-Weiermair M., Malli R, & Graier W.F. (2019). MICU1 controls cristae junction and spatially anchors mitochondrial Ca2+ uniporter complex. Nature Communications, 10, 3732.
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