We purchased CGP 55845A, DNQX, and DL-AP5 from Tocris Biosciences (Ellisville, MI, USA), recombinant mouse IL-1β from Biolegend (San Diego, CA, USA), recombinant human IL-1ra from Peprotech (Rocky Hill, NJ, USA), and TTX from Calbiochem (San Diego, CA, USA). We obtained ethanol from Remet (La Mirada, CA, USA).
Recombinant mouse il 1β
Manufactured by BioLegend
Sourced in United States
Recombinant mouse IL-1β is a cytokine that is produced and secreted by various cell types, including monocytes, macrophages, and dendritic cells. It plays a crucial role in the regulation of immune and inflammatory responses.
Automatically generated - may contain errors
Lab products found in correlation
Cgp 55845a, by Bio-Techne (3 mentions)
Dl ap5, by Bio-Techne (2 mentions)
Recombinant human il 1ra, by Thermo Fisher Scientific (2 mentions)
Rat anti mouse cd 11b antibody, by Bio-Rad (1 mentions)
Anti pro il 1β, by Abcam (1 mentions)
Anti 3 nitrotyrosine 3 nt, by Abcam (1 mentions)
Z yvad, by R&D Systems (1 mentions)
Il 1β elisa kit, by R&D Systems (1 mentions)
Cell culture ingredients, by Thermo Fisher Scientific (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Anti iba1, by Merck Group (1 mentions)
Mouse il 17a elisa kit, by BioLegend (1 mentions)
Cd11b pe m1 70, by BioLegend (1 mentions)
Fitc tnf α mp6 xt22, by BioLegend (1 mentions)
Tnf α, by BioLegend (1 mentions)
Interleukin 6 (il 6), by BioLegend (1 mentions)
Il 17a, by BioLegend (1 mentions)
6 protocols using recombinant mouse il 1β
1
Neurotransmission Modulation Assay
2
Neuroinflammation and α-Synuclein Pathology
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LPS (Escherichia coil O111:B4) used for cell culture studies was purchased from Calbiochem (San Diego, CA; cat# 437627) and for animal studies was purchased from Sigma-Aldrich (St. Louis, Mo; cat# L3012). Anti-tyrosine hydroxylase (TH) and anti-Iba-1 were purchased form EMD Millipore (Burlington, MA) and Wako (Richmond, VA), respectively. The secondary antibodies were purchased from Vector Laboratories (Burlingame, CA). The rat anti-mouse CD-11b antibody was purchased from abD Serotec (Raleigh, NC, cat# MCA711G). Anti-pro-IL-1β, anti-alpha-synuclein (α-synuclein), and anti-3-Nitrotyrosine (3-NT) antibodies were purchased from Abcam (Cambridge, MA). Mouse interleukin-1 receptor antagonist (IL-1Ra), NLRP3 inhibitor MCC950, caspase-1 inhibitor Z-YVAD, IRE1α (inositol-requiring enzyme 1α) inhibitor 4μ8C, TNF-α, and IL-1β ELISA kits were purchased from R&D Systems (Minneapolis, MN). Tauroursodeoxycholic acid (TUDC) was from Selleckchem (Houston, TX). Mouse IL-1β pro-form ELISA kit was from eBioScience (San Diego, California). Recombinant mouse IL-1β was from BioLegend (San Diego, CA). Cell culture ingredients were obtained from Invitrogen (San Diego, CA). All other reagents came from Sigma Chemical Co. (St. Louis, MO).
3
Pharmacological Manipulation of Neuroinflammation
4
Flow Cytometry and ELISA Immunoassays
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Fluorochrome-conjugated monoclonal antibodies (mAbs), including PE-CD11b (M1/70, 101207, 1:400), PerCP/Cy5.5-IL-17A (TC11-18H10.1, 506919, 1:100), PE/Cy5-Gr-1 (RB6-8C5, 108409, 1:600), APC-CD4 (GK1.5, 100412, 1:400), FITC-TNF-α (MP6-XT22, 506304, 1:100), and APC/Fire750-CD4 (GK1.5, 100460, 1:100) were purchased from BioLegend (San Diego, CA), while PE-IL-17A (eBio17B7, 12-7177-81, 1:100) was purchased from eBioscience (San Diego, CA). Anti-mouse IL-17A neutralizing monoclonal antibody (17F3, BP0173) was purchased from BioXCell (West Lebanon, NH). Recombinant mouse IL-1β, IL-6, IL-17A, and TNF-α were purchased from BioLegend. Mouse IL-17A ELISA kit was purchased from BioLegend. Mouse IL-17A ELISPOT kit was purchased from R & D Systems (Minneapolis, MN). Native chicken and bovine collagen type II were purchased from Sigma-Aldrich (St. Louis, MO) and Chondrex (Redmond, WA), respectively.
5
MyD88-Targeting Compound Synthesis
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We purchased CGP 55845A, DNQX, and DL-AP5 from Tocris Biosciences (Ellisville, MI, USA) and recombinant mouse IL-1β from BioLegend (San Diego, CA, USA). We obtained ethanol from Remet (La Mirada, CA, USA). Hydrocinnamoyl-L-valyl-pyrrolidine (AS-1)—a low-molecular-weight MyD88 mimetic—was synthesized as previously described [42 (link)].
6
Isolation and Culture of Murine Abdominal Stromal Cells
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Primary cultures of abdominal stromal cells were prepared from peritoneal wall biopsies and mesentery of adult mice. The tissues were washed in PBS and digested in 0.25% trypsin-EDTA solution for 50 min at 37°C with gentle agitation. The mixture was filtered on a 70-µm cell strainer and centrifuged at 1,500 rpm for 5 min. Cells were cultured in DMEM supplemented with 15% heat-inactivated FBS, penicillin (100 U/ml), streptomycin (100 µg/ml) and amphotericin B (250 ng/ml) until confluence. Cells were subcultured in DMEM containing 10% FBS and used at passage 3. For experimentation, 1.5×105 cells were seeded in 6-well plates, starved for 24 h and incubated for 3 h with 100 µg/ml PTX, 10 ng/ml recombinant mouse IL-1β (BioLegend) or PBS as control.
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