Amg9810

Manufactured by Merck Group

Sourced in United States

AMG9810 is a lab equipment product from Merck Group. It is a chemical compound used as a tool in scientific research and experimentation. The core function of AMG9810 is to serve as a research instrument, without further interpretation of its intended use.

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Lab products found in correlation

19 protocols using amg9810

Pharmacological Characterization of Neuroactive Compounds

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Histamine dihydrochloride, chloroquine diphosphate salt, VUF 8430 dihydrobromide, histamine trifluoromethyl toluidide (HTMT), osthole, capsaicin, and AMG9810 were obtained from the Sigma-Aldrich Corp. (St. Louis, MO, USA). With the exception of histamine and chloroquine, which were dissolved in water, all the other drugs were dissolved in DMSO. When the drugs were used in the behavior experiments, the drugs were diluted in saline, then the calcium imaging and the electrophysiological experiments, all the drugs were diluted in normal perfusion solution, the final concentration of DMSO or water did not exceed 0.5%.

Yang N.N., Shi H., Yu G., Wang C.M., Zhu C., Yang Y., Yuan X.L., Tang M., Wang Z.L., Gegen T., He Q., Tang K., Lan L., Wu G.Y, & Tang Z.X. (2016). Osthole inhibits histamine-dependent itch via modulating TRPV1 activity. Scientific Reports, 6, 25657.

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Niacin-induced TRPV1 Activation in Mice

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Wild-type C57BL/6 mice were obtained from Charles River Laboratories (Wilmington, MA). TRPV1^–/– (KO) mice were obtained from Jackson Laboratories (Sacramento, CA). All mice were adult males (3–6 months of age). For the experiments, mice were anesthetized using pentobarbital (Sigma-Aldrich, St. Louis, MO) at a dose of 60 mg/kg dissolved in 10% ethanol given by intraperitoneal (IP) injection. Niacin (Sigma-Aldrich) was administered by IP injection at a dose of 30 mg/kg dissolved in 0.9% saline (15 mg/mL). The TRPV1 antagonist AMG9810 [(E)-3-(4-t-Butylphenyl)-N-(2,3-dihydrobenzo[b][1,4] dioxin-6-yl)acrylamide] (Sigma-Aldrich) was administered by IP injection at doses of 20 and 40 mg/kg in PEG400 (20 mg/mL). The TRPV1 agonist capsaicin (Sigma-Aldrich) was administered topically at a dose of 0.2 mg in acetone (20 mg/mL).

Clifton H.L., Inceoglu B., Linlin M., Zheng J, & Schaefer S. (2015). TRPV1 Channels Are Involved in Niacin-induced Cutaneous Vasodilation in Mice. Journal of cardiovascular pharmacology, 65(2), 184-191.

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TRPV1 Antagonist Effects in CCI Mice

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Pirt^−/− or WT mice in CCI treated or untreated groups underwent intraperitoneal injection of AMG9810 (Sigma-Aldrich, St. Louis, Missouri, United States; TRPV1 antagonist, 3 mg/kg) or vehicle as before [21 (link), 22 (link)]. 30 minutes later, the mouse was used for behavior tests.

Wang C., Gu L., Ruan Y., Gegen T., Yu L., Zhu C., Yang Y., Zhou Y., Yu G, & Tang Z. (2018). Pirt Together with TRPV1 Is Involved in the Regulation of Neuropathic Pain. Neural Plasticity, 2018, 4861491.

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Pharmacological Characterization of ASIC Channels

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Drugs were obtained from Sigma Chemical Co. (St. Louis, MO, USA), including hydrochloric acid, ET-1, BQ-123, BQ-778, amiloride, APETx2, capsaicin, and AMG9810. Different pH values were configured with hydrochloric acid and external solution. Working ET-1 and other drugs were freshly prepared in normal external solution and held in a series of independent reservoirs. The pipette tips connecting the reservoirs were positioned ∼30 μm away from the recorded neurons. The application of each drug was driven by gravity and controlled by the corresponding valve. In some experiments where GDP-β-S (Sigma) or GF109203X (RBI) was applied for intracellular dialysis through recording patch pipettes, they were dissolved in the internal solution before use. To ensure that the cell interior was perfused with the dialysis drug, there was at least a 30 min interval between the establishment of whole-cell access and current measurement. To functionally characterize ASIC activity, we used AMG9810 (5 μM) to block TRPV1 in the extracellular solution [36 (link)].

Wu L., Liu T.T., Jin Y., Wei S., Qiu C.Y, & Hu W.P. (2020). Endothelin-1 enhances acid-sensing ion channel currents in rat primary sensory neurons. Acta Pharmacologica Sinica, 41(8), 1049-1057.

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Mechanisms of Inflammatory Pain Modulation

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Carrageenan and PGE2 were purchased from Sigma-Aldrich (St. Louis, MO, United States). The PKCε agonist ψεRACK (peptide-sequence HDAPIGYD with a membrane permeable sequence) was synthesized by Bankpeptide (Hefei, China). The PKCε inhibitor PKCεV1-2 was purchased from Calbiochem, Milipore Sigma (Darmstadt, Germany). The TRPV1 antagonist capsazepine (CPZ) and AMG9810 were purchased from Sigma-Aldrich (St. Louis, MO, United States) and Abcam (United States). Morphine was purchased from Northeast Pharmaceutical Group Shenyang No.1 Pharmaceutical Co., Ltd. (Shenyang, China).
A stock solution of PGE2 (1 μg/μl) was prepared in 10% ethanol and dissolved in normal saline (NS) to a concentration of 100 ng/25 μl immediately before injection. Car was dissolved in NS to a concentration of 2% and stored. AMG9810 was prepared in Dimethyl sulfoxide as a stock and diluted in PBS to a concentration of 0.8 mg/25 μl before injection. The peptide ψεRACK, capsazepine, and PKCεV1-2 were prepared in NS to a concentration of 1 μg/25 μl immediately before injection. Morphine (10 mg/kg) was injected intraperitoneally.
The use of all the drugs in this study was based on previous studies.

Fang J., Wang S., Zhou J., Shao X., Sun H., Liang Y., He X., Jiang Y., Liu B., Jin X., Fang J, & Du J. (2021). Electroacupuncture Regulates Pain Transition Through Inhibiting PKCε and TRPV1 Expression in Dorsal Root Ganglion. Frontiers in Neuroscience, 15, 685715.

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Mechanical Hypersensitivity in Neuropathic Pain

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Mechanical hypersensitivity was tested in mice at 24 weeks after SNI or sham surgery or 24 h after injection of complete Freund’s adjuvant (CFA), before and 30 min after intraplantar application of the following drugs, each in a volume of 20 µl: diclofenac (D6899, Sigma Aldrich; 50 µg), celecoxib (PHR1683, Sigma Aldrich; 150 µg), AMG 9810 (A2731, Sigma Aldrich; 20 µg), AP-18 (A7232, Sigma Aldrich; 4.2 µg) and tanezumab (anti-NGF antibody; TAB-111, CreativeBiolabs; 30 µg).

Gangadharan V., Zheng H., Taberner F.J., Landry J., Nees T.A., Pistolic J., Agarwal N., Männich D., Benes V., Helmstaedter M., Ommer B., Lechner S.G., Kuner T, & Kuner R. (2022). Neuropathic pain caused by miswiring and abnormal end organ targeting. Nature, 606(7912), 137-145.

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Antagonizing TRPA1 and TRPV1 Receptors

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Complete Freund adjuvant (CFA; Sigma) was emulsified in an equal volume of isotonic saline. AP18 (40 nmol/μl; Sigma), a TRPA1 antagonist, was dissolved in PBS containing 1% DMSO and 10% Tween-80. AMG 9810 (10 nmol/μl; Sigma), a TRPV1 antagonist, was dissolved in PBS containing 5% DMSO and 10% Tween-80. Antibodies against 13(S)-hydroxyoctadecadienoic acid (HODE) and 9(S)-HODE (Oxford Biomedical Research; 1 mg/ml) or goat IgG (a negative control; Sigma; 1 mg/ml) were suspended in PBS. The specificity of these antibodies was previously validated (Spindler et al., 1996 (link)). Catalase (Sigma; bovine liver; 300 units in 20 μl) was dissolved in PBS. Heat-inactivated Catalase (denatured by boiling for 10 min) was used as a control. The concentration and dose of Catalase was determined previously (Trevisan et al., 2014 (link)). For intramuscular injection, mice were briefly anesthetized using 3% isoflurane.

Wang S., Brigoli B., Lim J., Karley A, & Chung M.K. (2018). Roles of TRPV1 and TRPA1 in Spontaneous Pain from Inflamed Masseter Muscle. Neuroscience, 384, 290-299.

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Masseter Muscle Hypersensitivity Model

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Mechanical hypersensitivity in the masseter muscle was assessed under CFA-induced inflammatory condition as described previously.^{7 (link)} A series of calibrated Von Frey filaments (1–125 g) were applied to the region over the masseter muscle. An active withdrawal of the head from the filament application was defined as a positive response. Each VF filament was applied five times and the response frequencies ((number of responses/number of stimuli) ×100%) to a range of filament forces were determined. After a non-linear regression analysis, an EF50 value, the filament force (g) necessary to produce a 50% response frequency, was determined. Mechanical sensitivity of the masseter muscle was determined before and three days after the CFA injection in the masseter muscle. The effect of a TRPV1 antagonist, AMG9810 (Sigma-Aldrich, St. Louis, MO), on mechanical sensitivity was examined on three days following CFA injection. On test day, AMG9810 (1 µmol in 50 µl) or the same volume of vehicle was administered directly in the masseter muscle under anesthesia using isoflurane. The post AMG9810 or vehicle effect was measured 30 min, 1 h, and 24 h after the drug injection.

Chung M.K., Park J., Asgar J, & Ro J.Y. (2016). Transcriptome analysis of trigeminal ganglia following masseter muscle inflammation in rats. Molecular Pain, 12, 1744806916668526.

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Pharmacological Agents for ESCC Research

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Capsaicin, AMG9810, and ruthenium red were obtained from Sigma‐Aldrich (St. Louis, MO, USA); O1821 and tranilast were purchased from Cayman (Ann Arbor, Michigan, USA) and TargetMol Chemical (Boston, MA, USA), respectively. The chemicals were dissolved in DMSO (the maximal final concentration of DMSO was never exceeded 0.1% throughout the study) and diluted in PBS or extracellular solutions (pH 7.4) to obtain the desired concentrations. Agonists and antagonists were used at the concentrations based on our pre‐experiments on ESCC cells and referred to the EC₅₀ or IC₅₀ as recommended by the suppliers (Table 1). Matching volumes of DMSO were used as controls.

Huang R., Wang F., Yang Y., Ma W., Lin Z., Cheng N., Long Y., Deng S, & Li Z. (2019). Recurrent activations of transient receptor potential vanilloid‐1 and vanilloid‐4 promote cellular proliferation and migration in esophageal squamous cell carcinoma cells. FEBS Open Bio, 9(2), 206-225.

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Osthole's Neuromodulatory Mechanisms

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Osthole was obtained from Shanghai YuanYe Biotechnology Co., Ltd. (Shanghai, China). Histamine, Histamine trifluoromethyl toluidide (HTMT), capsaicin, VUF 8430 dihydrobromide, and AMG9810 were obtained from Sigma-Aldrich Corp. (St. Louis, MO, USA). All drugs were dissolved in dimethyl sulfoxide (DMSO) or saline. The drugs were diluted in saline used in the scratching experiments or normal perfusion solution in the calcium imaging experiments. The final concentrations of DMSO or water did not exceed 0.5%.

Yang N., Ju Y., Huang D., Ling K., Jin H., Liu J., Ma J., Chen Y., Zhang Y., Zhu C., Yang Y., Tang Z., Chen X, & Wu G. (2021). Desensitization of TRPV1 Involved in the Antipruritic Effect of Osthole on Histamine-Induced Scratching Behavior in Mice. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 4012812.

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