Blood samples were collected into 1.5 Eppendorf tubes or BD Vacutainer® SST™ serum separation tubes (BD, Franklin Lakes, New Jersey), by cardiac puncture from adults (p=70 ±5 days) ephrin-A5−/− (n=13), and wild-type (n=15) male mice and allowed to clot. The blood was then centrifuged to isolate the serum, which was then extracted using diethyl ether (Sigma, St. Louis, MO). Testosterone concentrations were measured using a commercially available competitive ELISA kit (Cayman Chemical Ann Arbor, MI, Cat No.582701).
Vacutainer sst serum separation tubes
Manufactured by BD
Sourced in United States, United Kingdom
The BD Vacutainer SST Serum Separation Tubes are laboratory collection tubes designed to facilitate the separation of serum from whole blood samples. These tubes contain a gel barrier that helps isolate the serum portion of the blood sample during centrifugation.
Automatically generated - may contain errors
Lab products found in correlation
Nacitrate cpt tubes, by BD (2 mentions)
Diethyl ether, by Merck Group (1 mentions)
Competitive elisa kit, by Cayman Chemical (1 mentions)
Sepmate tube, by STEMCELL (1 mentions)
Hs crp elisa kit, by Biomatik (1 mentions)
Bioplex 2200 system, by Bio-Rad (1 mentions)
Eba 200, by Hettich (1 mentions)
Multiplex assay, by Merck Group (1 mentions)
Luminex analyses, by R&D Systems (1 mentions)
22 protocols using vacutainer sst serum separation tubes
1
Testosterone Quantification in Ephrin-A5 Knockout Mice
2
Serum Carotenoid Analysis by HPLC
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Fasting (overnight fast > 9 h) blood samples were collected at 0, 3, and 6 months for XC serum analysis. Blood samples were collected by standard venepuncture techniques in 9 mL blood collection tubes (BD Vacutainer SST Serum Separation Tubes) containing a “Z Serum Sep Clot Activator”. Collection tubes underwent thorough mixing of the clot activator. The blood samples were left for 30 min at room temperature to clot and then centrifuged at 725 g for 10 min in a GruppeGC12 centrifuge (Desaga Sarstedt) to separate the serum from the whole blood. Following centrifugation, serum was transferred to light-resistant microtubes and stored at circa −80 °C until the time of batch analysis. Serum carotenoid analysis was performed by high performance liquid chromatography (HPLC), using a method previously described by our laboratory [31 (link)]. The calibration lines used, as well as the lower and upper limits of quantification (LLOQ and ULOQ respectively), were as in the cited work. Serum carotenoid analysis was completed in sixteen independent batches, with a maximum intra-day precision of 7.28%, measured as RSD, and an inter-day precision of 3.16% (RSD).
3
Serum Isolation from Venous Blood
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For experiments requiring use of fresh serum, venous blood (5–15 ml) was collected in BD Vacutainer SST serum separation tubes (BD Biosciences, 368013) at the time of blood collection for PBMCs from either the same blood donor (autologous) or a separate donor (non-autologous). Serum was isolated according to the manufacturer’s protocol in which collected blood was mixed with clotting agents by inversion of the tubes and incubated at RT for 30 min before centrifugation at 1200 × g for 10 min at RT. Serum was collected from above the polymer gel plug. An additional centrifugation at 1200 × g for 10 min at RT was used as necessary to remove any remaining RBCs.
4
Serum Isolation from Venous Blood
5
Longitudinal SARS-CoV-2 Antibody Levels
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Venous blood was drawn from healthy individuals using standard phlebotomy techniques with approval of the UofL Institutional Review Board, IRB number 14.0661. Consented donors, recently immunized with Pfizer/BioNTech COVID vaccine BNT162b2 as part of a campus-wide vaccination drive, volunteered to provide 4–5 mL peripheral blood at various times following immunization through 240 days (8 months). Blood samples were collected from all individuals (N = 5) into BD Vacutainer SST serum separation tubes (BD Biosciences, Cat #368013), inverted, then incubated at room temperature for 30 minutes prior to centrifugation at 1200 × g for 10 minutes (room temperature). The sera were transferred to fresh tubes and centrifuged again to remove residual red blood cells. Coded serum samples were aliquoted, 0.2 mL per 1.5 mL Eppendorf tubes (Eppendorf®, 0.5 mL, Cat #022363611), and stored at -80°C until use.
6
Blood Serum Collection and Storage
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Blood was drawn after overnight fasting using BD Vacutainer®SST Serum Separation Tubes (BD, Plymouth, UK). After collection, tubes were inverted five times, allowed 30 min clotting time, and centrifuged at room temperature for 15 min at 1300× g in a fixed-angle centrifuge. The serum was removed, aliquoted and stored at −80 °C for further use.
7
Serum Biomarker Profiling in Rheumatoid Arthritis
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Venous blood was collected into BD Vacutainer SST™ serum separation tubes (BD Biosciences, US) by a trained phlebotomist or study nurse, allowed to clot for 35 minutes and then centrifuged to separate the serum. Aliquots of sera were stored at −20°C until required for serology assays. C-reactive protein (CRP) levels were measured using a human high-sensitivity CRP (hs-CRP) ELISA kit (Biomatik, Canada) as per the manufacturer’s instructions. ACPA titer was determined using either the anti-CCP2 on a BioPlex® 2200 System (Bio-Rad, US) or anti-CCP3 kits (Inova Diagnostics Inc, San Diego, CA). ACPA seropositivity status was considered negative if the titer was below manufacturer’s standardized assay cutoff (< 20U/mL, also known as upper limit normal or ULN) (13 (link)). For peripheral blood mononuclear cells (PBMC), venous blood was collected into heparinized vacutainers (BD) and isolation was performed using SepMate™ tubes (StemCell Technologies) as per the manufacturer’s protocol. Isolated PBMCs were cryopreserved in a freezing medium (90% fetal bovine serum and 10% DMSO) and stored in liquid nitrogen.
8
Serum Carotenoid Analysis by HPLC
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Blood samples were collected by standard venipuncture technique in 9-mL blood collection tubes (BD Vacutainer SST Serum Separation Tubes) containing a “Z Serum Sep Clot Activator.” Collection tubes underwent thorough mixing of the clot activator. The blood samples were left for 30 minutes at room temperature to clot and then centrifuged at 725g for 10 minutes in a Hettich EBA 200S centrifuge (Andreas Hettich GmbH & Co. KG, Tuttlingen, Germany) to separate the serum from the whole blood. Following centrifugation, serum was transferred to light-resistant microtubes and stored at circa −80°C until the time of batch analysis. Serum carotenoid analysis was performed by high-performance liquid chromatography, using a method previously described by our laboratory.28 Calibration lines used, as well as lower and upper limits of quantification are as in the cited work. Serum carotenoid analysis was completed in 16 independent batches, with a maximum intraday precision of 7.28%, measured as the Residual Standard Deviation (RSD), and an interday precision of 3.16% (RSD).
9
Serum Testosterone Quantification Protocol
10
Rat Renal Function Evaluation
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At the end‐point of the experiment, all the rats were kept in metabolic cages for 24‐hrs urine collection. Rat blood samples were collected from abdominal aorta and moved into BD Vacutainer® SST™ Serum Separation Tubes (Becton‐Dickinson, Franklin Lakes, NJ, USA). After centrifugation, the serum was then separated and stored at −80°C. Serum and urine creatinine were measured by an automatic biochemical analyser (Hitachi 7600, Tokyo, Japan) to assess the alteration of renal function. Creatinine clearance (Clcr) was calculated by the formula ‘24‐hr creatinine clearance = (urine creatinine concentration × urine flow rate)/serum creatinine concentration and expressed as ml/min.
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