Anti mouse rabbit secondary antibody for western blot

Anti-GAPDH (6c5), and anti-AR antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-MMP1 and Anti-MMP13 antibodies were purchased from Bioss (bs-4597R, bs-0575R, Woburn, MA). Anti-Human IL-8 antibody was purchased from Peprotech (500-p28, Rocky Hill, NJ). Recombinant Human IL-8 was purchased from R and D Systems (208-IL-010, Minneapolis, MN). MMP1 inhibitor was purchased from EMD Millipore (#444250, Gibbstown, NJ) and MMP13 inhibitor was purchased from Sigma (CL-82198, St Louis, MO). Polyvinylidene defluoride membrane (PVDF) was from Thermo Fisher Scientific (Rochester, NY). Anti-mouse/rabbit secondary antibody for Western blot was from Invitrogen (Grand Island, NY).

GAPDH (6c5), β-actin (I-19), and AR (N-20) antibodies were purchased from Santa Cruz Biotechnology (Paso Robles, CA). MMP9 (ab38898) was from Abcam (San Diego, CA). SUZ12 (3737) was from Cell Signaling (Boston, MA). Human IL6 protein was from PROSPEC Corp (Houston, TX). IL6 and IL8 neutralizing antibody was from R&D (Minneapolis, MN). Crystal violet was from Fisher Scientific Company (Grand Island, NY). Anti-mouse/rabbit secondary antibody for Western Blot was from Invitrogen (Grand Island, NY).

GAPDH (6c5), AR (N-20) antibodies, and PPARγ-Antagonist III were purchased from Santa Cruz Biotechnology. PPARγ, PDK1, PGC-1β, and MMP-9 antibody were purchased from One World Lab. Anti-mouse/rabbit secondary antibody for Western Blot was from Invitrogen. Normal rabbit IgG was also from Santa Cruz Biotechnology. The PPARγ-Antagonist III and Enz stock concentrations were 10 mM.