Anhydrous dichloromethane dcm

Distearoylphosphatidylethanolamine–polyethylene glycol (DSPE–PEG, PEG Mw of 550 and 2000 Da) were purchased from Avanti polar lipids (Alabama, USA). Distearoylphosphatidylethanolamine (DSPE) and hydrogenated soy phosphatidylcholine (HSPC) were purchased from Lipoid (Ludwigshafen, Germany). Triethylamine (99%), 2-(methacryloyloxy)ethyl phosphorylcholine (MPC), copper(i) bromide (CuBr, 99.999%), 2-bromoisobutyryl bromide (BIBB, 98%), N,N,N′,N′′,N′′-pentamethyldiethylenetriamine (PMDETA, 99%), bovine serum albumin (BSA), anhydrous dichloromethane (DCM), and anhydrous chloroform were purchased from Sigma-Aldrich (Israel). DiR (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide), DiI (1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate), fetal bovine serum (FBS), and RPMI-1640 medium were purchased from Thermo Fisher Scientific (UK). Hyaluronate-DyLight 755 (2.5 MDa) was purchased from Creative PEG Works (US).

Camptothecin (CPT), IR-820, 2-hydroxyethyl disulfide (HEDS), 1, 6-hexanediol, triphosgene, 4-nitrophenyl chloroformate, 4-dimethylaminopyridine (DMAP), and other reagents were purchased from Sigma-Aldrich (USA). The solvents anhydrous dimethylformamide (DMF), and anhydrous dichloromethane (DCM) were also acquired from Sigma-Aldrich (USA). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco. All other reagents and solvents were of analytical or chromatographic grade.

The following materials were used as received: methoxy polyethylene glycol 1000 Da (PEG₁₀₀₀) (Sigma-Aldrich), triethylamine (TEA) (99.5%, Sigma-Aldrich), pyridine (99.8%, Sigma-Aldrich) p-nitrophenyl chloroformate (pNPCF) (98%, Sigma-Aldrich), 2,5-dihydroxybenzoic acid (2,5-DHB) (Sigma-Aldrich), anhydrous dichloromethane (DCM) (Sigma-Aldrich), dimethyl sulfoxide (DMSO) (Acros Geel), K₂PtCl₄ (min. 42.4% Pt, Alfa Aesar), n-octanethiol (C8) (98%, Sigma-Aldrich), deuterium oxide (D, 99.96%, Cambridge Isotope Laboratories), and phosphate buffer (10x, Lief Technologies). Fourth generation amine terminated PAMAM dendrimers were purchased as 10% by weight solutions in methanol (Sigma-Aldrich, St. Louis, MO). Silica gel 60 A 230–400 mesh ATSM (Whatman Inc) and silica gel 60 F₂₅₄ plastic sheets (TLC) (Merck KGaA) were used for column and thin layer chromatography, respectively. Ultrapure water (≥18 MΩ·cm, Millipore Milli-Q) and 200 proof ethanol (Gold Shield Chemical Co.) were used for dilution and washing. Ultrapure N₂ (98%, Air Gas Co.) and H₂ (99.99%, Praxair, Inc.) were used for drying and flaming, respectively. Tungsten wire (d = 0.010 in., 99.95%, California Fine Wire Co.) was used to make STM tips. Au slugs (99.99%, Alpha Aesar Premion Co.) and mica (clear ruby muscovite, Mica New York Corp.) were used for Au thin film preparation.