Shyap1 2

Manufactured by Addgene

Sourced in United States

ShYAP1/2 is a plasmid that can be used to knockdown the expression of the YAP1 and YAP2 genes. It contains short hairpin RNA (shRNA) sequences targeting the YAP1 and YAP2 transcripts.

Automatically generated - may contain errors

Lab products found in correlation

Pcdna3, by Addgene (3 mentions) Secondary fluorescent antibodies, by Abcam (3 mentions) H e staining kit, by Abcam (2 mentions) Rock inhibitor y2763, by Merck Group (2 mentions) Tead1, by Santa Cruz Biotechnology (2 mentions) Gna13, by Santa Cruz Biotechnology (2 mentions) Rgs12 antibody ab1, by Merck Group (2 mentions) Pdz domain peptides of rgs12, by GenScript (2 mentions) Gpcr activator lpa, by Merck Group (2 mentions) Gpcr inhibitor ki6425, by Merck Group (2 mentions) Rho gtpases inhibitor c3, by Merck Group (2 mentions) Tead1 sirna and controls, by Santa Cruz Biotechnology (2 mentions) Pcdna3.1 gfp yap, by Addgene (2 mentions) Pet gst yap, by Addgene (2 mentions) Pcdna3.1 rhoa, by Addgene (2 mentions) Shrgs12 1, by Applied Biological Materials (2 mentions) Shrgs12 2, by Applied Biological Materials (2 mentions) Shrgs12 3, by Applied Biological Materials (2 mentions) Edta free cocktail inhibitor tablets, by Thermo Fisher Scientific (2 mentions) Fugene hd transfection reagent, by Promega (2 mentions)

4 protocols using shyap1 2

Investigating YAP Signaling Pathway

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Antibody against pYAP127 (D9W2I), YAP (D8H1X) was purchased from Cell Signaling Technology. The secondary fluorescent antibodies and H&E staining kit were from Abcam. Glut1 inhibitor Bay‐876 was obtained from MCE Med Chem Express. The fluorescent glucose analog 2‐(N‐(7‐nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl) amino)‐2‐deoxyglucose (2‐NBDG) was from Cayman Chemical Company. TEAD1 siRNAs were purchased from Santa Cruz Biotechnology. TRAP staining kit was purchased from Sigma. Plasmids pRL‐TK, pcDNA3.1, pcDNA3.1‐flag‐YAP, and shYAP1/2 were obtained from Addgene. The transfection was performed as we previously reported.¹, ⁵⁴, ⁵⁵

Li Y., Yang S., Liu Y, & Yang S. (2022). Deletion of Trp53 and Rb1 in Ctsk‐expressing cells drives osteosarcoma progression by activating glucose metabolism and YAP signaling. MedComm, 3(2), e131.

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Hippo Signaling Pathway Characterization

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Trp53 (1C12), YAP (D8H1X), GAPDH antibodies, and Hippo Signaling Antibody Sampler Kit were purchased from Cell Signaling Technology. Antibody against Rb1 was purchased from Santa Cruz Biotechnology. The secondary fluorescent antibodies and H&E staining kit were from Abcam. DAB Substrate Kit was ordered from Vector Laboratories Inc. Plasmids pRL-TK, 8xGTIIC-luciferase, and shYAP1/2 were obtained from Addgene. The transfection reagents (FuGENE^® HD) were obtained from Promega Corporation.

Li Y., Yang S., Liu Y, & Yang S. (2022). Mice with Trp53 and Rb1 deficiency in chondrocytes spontaneously develop chondrosarcoma via overactivation of YAP signaling. Cell Death & Disease, 13(6), 570.

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Dissecting YAP/TEAD Signaling Pathway

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Antibodies against p127YAP (D9W2I), YAP (D8H1X), MST1, pMST (E7UD1), LATS1 (C66B5), pLATS1, Lamin B1 (D9V6H) and GAPDH were from CST. Antibodies of Ezrin, GNA12, GNA13, RhoA, flag, TEAD1, GST and GFP were from Santa Cruz Biotechnology. RGS12 antibody (ab1) was from Sigma. The secondary fluorescent antibodies were from Abcam. PDZ domain peptides of RGS12 were purchased from GenScript. GPCR activator LPA, GPCR inhibitor Ki6425, Rho GTPases inhibitor C3 and Rock inhibitor ‎Y2763 were obtained from Sigma. Doxorubicin hydrochloride (DOX), methotrexate 4-Amino-10-methylfolic acid hydrate (MTX) and EDTA-free cocktail inhibitor tablets were all obtained from Fisher Scientific^™. TEAD1 siRNA and controls were from Santa Cruz Biotechnology. FuGENE^® HD Transfection Reagent was purchased from Promega Corporation. The primers used for the quantification are listed in Supplementary Table 1.
The following plasmids: pRL-TK was generously provided by Dr. Zhen Zhang (University of Pennsylvania, Philadelphia, PA, USA); pcDNA3.1, shYAP1/2, pcDNA3.1-GFP-YAP, PET-GST-YAP and pcDNA3.1-RhoA were obtained from Addgene. Three human shRGS12 lentivectors (shRGS12–1, shRGS12–2 and shRGS12–3; Catalog # i019000) were ordered from ABM. pcDNA3.1-flag-RGS12 (flag-RGS12) and the pcDNA3.1-flag-RGS12 mutant with the deletion of the PDZ domain vectors (flag-RGS12ΔPDZ) were constructed in our lab.

Li Y., Liu M., Yang S., Fuller A.M., Eisinger T.S, & Yang S. (2021). RGS12 is a novel tumor suppressor in osteosarcoma that inhibits YAP-TEAD1-Ezrin signaling. Oncogene, 40(14), 2553-2566.

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Dissecting YAP/TEAD Signaling Pathway

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Li Y., Liu M., Yang S., Fuller A.M., Eisinger T.S, & Yang S. (2021). RGS12 is a novel tumor suppressor in osteosarcoma that inhibits YAP-TEAD1-Ezrin signaling. Oncogene, 40(14), 2553-2566.

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