PLP, l -Orn, α-KG, 2-aminobenzaldehyde, dimethyl sulphoxide (DMSO), isopropyl-β-d -thiogalactoside (IPTG), phenylhydrazine hydrochloride and phenylmethylsulfonyl fluoride (PMSF) were purchased from Sigma. 1,8-Anilino-naphthalene sulfonic acid (ANS) was purchased from Molecular Probes. All other chemicals were of the highest purity available. The pYES2.1::hOAT expression plasmid coding for the complete cDNA of hOAT [22 (link)] was kindly provided by Prof. Leonardo Salviati of the University of Padova (Italy).
1 8 anilino naphthalene sulfonic acid ans
Manufactured by Thermo Fisher Scientific
Sourced in United States
1,8-anilino-naphthalene sulfonic acid (ANS) is a fluorescent dye used as a probe in various biochemical and biophysical studies. It is a small molecule that exhibits a significant increase in fluorescence upon binding to hydrophobic environments, such as the interior of proteins. ANS can be used to study protein folding, ligand binding, and membrane interactions.
Automatically generated - may contain errors
Lab products found in correlation
2 aminobenzaldehyde, by Merck Group (3 mentions)
L orn, by Merck Group (2 mentions)
Phenylmethylsulfonyl fluoride, by Merck Group (2 mentions)
Dimethyl sulphoxide, by Merck Group (2 mentions)
Isopropyl β d thiogalactoside, by Merck Group (2 mentions)
Phenylhydrazine hydrochloride, by Merck Group (1 mentions)
Isopropyl β d thiogalactoside iptg, by Merck Group (1 mentions)
Pyridoxine, by Merck Group (1 mentions)
Pyridoxamine, by Merck Group (1 mentions)
Pyridoxal, by Merck Group (1 mentions)
3 protocols using 1 8 anilino naphthalene sulfonic acid ans
1
Heterologous Expression of Human Organic Anion Transporter
2
Protein Labeling and Characterization
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L-Orn, α-KG, 2-aminobenzaldehyde, dimethyl sulphoxide, isopropyl-β-D-thiogalactoside, pyridoxine (PN), pyridoxamine (PM), pyridoxal (PL) were purchased from Sigma-Aldrich. 1,8-anilino-naphthalene sulfonic acid (ANS) was purchased from Molecular Probes. Growth media and additives were purchased from Gibco. PEGylated bis (sulfosuccinimidyl) suberate [BS (PEG) 5] was purchased from ThermoFisher. All other chemicals were of the highest purity available.
3
Enzymatic Assay Protocol
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L-Orn, α-KG, 2-aminobenzaldehyde, dimethyl sulphoxide, isopropyl-β-D-thiogalactoside, PLP, phenylmethylsulfonyl fluoride (PMSF) were purchased from Merck & Co (Rahway, NJ, USA), while 1,8-anilino-naphthalene sulfonic acid (ANS) was purchased from Molecular Probes (Eugene, OR, USA). All other chemicals were of the highest purity available.
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