Uvgl 58

The fluorescent spots of PlAMV-GFP infection in N. benthamiana plants were visualized with a hand-held UV lamp (UVGL-58; Funakoshi, Japan) in a dark room and photographed with a digital camera EOS90D (Canon, Tokyo). PlAMV-GFP infection in protoplasts was observed using a BX53 microscope (Olympus, Tokyo, Japan) and a U-HGLPS light source (Olympus) as described previously (Saito et al., 2021 (link)), and virus infection in A. thaliana was observed as described previously (Matsuo et al., 2019 (link)). The number of fluorescent spots in the observed images was quantified using ImageJ software (https://imagej.nih.gov/ij/download.html).

Photo-crosslinking of a recombinant GST (50 μM) with the caged binder (0.50 mM) was carried out with the irradiation of a 365 nm light by using a handheld UV lamp (6 W, UVGL-58, 100 V; Funakoshi, Japan) for 5, 10, or 30 sec, or 1–20 min in a solution containing D-PBS (pH 7.4)/DMSO (v/v, 4:1, total volume 10 μL) at room temperature. A small portion of the resulting product was then subjected to 15% SDS-PAGE (shown in Figure 3). After the electrophoresis, the crosslinked protein was visualized by in-gel fluorescence imaging using a fluoroimager (FMBIO III-SC01, Hitachi, Japan). More conveniently, it could be also visualized by the naked eye or through a smartphone using the handheld UV lamp as an excitation source. GST was also visualized by a rapid stain CBB kit (Nacalai, Japan), and gel imaging was performed by a conventional gel imager (Gel Doc XRS+; Bio-Rad, Hercules, CA, USA).