Mbs726298

The fasting glucose and insulin levels were assessed using commercially available kits formulated specifically for rats (#90010, Crystal Chem, Elk Grove Village, IL, USA and #DIGL-100, BioAssay Systems, Hayward, CA, USA) following each supplier’s instructions. In addition, we calculated the values from the homeostatic model assessment of β-cell function (HOMA-β) for each animal using the previously published equation [30 (link)]: HOMA-β = (Fasting insulin (ng/mL) × 20)/(Fasting glucose (mg/dL) − 3.5). Levels of total cholesterol (CHOL) and triglyceride (TG) serum levels were determined using multi-enzyme-based kits (#MBS726298, MyBioSource, San Diego, CA, USA, and #ECCH-100, BioAssay Systems, Hayward, CA, USA, respectively). Serum and hepatic levels of high-/low-density lipoprotein cholesterol (LDL-c/HDL-c were assayed using a special colorimetric kit (#E2HL-100 BioAssay Systems, CA, USA). Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase were analyzed using assay kits (#EALT-100 and EASTR-100, BioAssay Systems, CA, USA). All procedures were performed according to the manufacturer’s instructions for each kit (n = 8 samples/group).

As previously described, an ELISA kit for triglycerides (TGs) with an established protocol by (MyBioSource, USA) was used to quantitatively assess the amount of rat TG (lipid profile marker) (TG; cat. no. MBS726298; MyBioSource, Inc.). The detection antibody used was a (biotinylated polyclonal) anti-rat TG antibody, whereas the pre-coated antibody was a (polyclonal anti-TG antibody). After adding samples and biotinylated antibodies to corresponding wells of an ELISA plate, the reaction will occur, and the color will develop. Then the stop solution will be added to measure the developed color at (450 nm).