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Hrp conjugated goat anti rabbit igg sc 2054

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HRP-conjugated goat anti-rabbit IgG (sc-2054) is a secondary antibody produced in goat and conjugated with horseradish peroxidase (HRP). This antibody is designed to detect and bind to rabbit immunoglobulin G (IgG) in various immunoassays.

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Lab products found in correlation

Enhanced chemiluminescence detection system, by Cytiva (2 mentions) Anti bcl11b, by Cell Signaling Technology (2 mentions) Hrp conjugated rat anti mouse igg kappa light chain, by BD (2 mentions) Sc 7963, by Santa Cruz Biotechnology (1 mentions) Sc 5565, by Santa Cruz Biotechnology (1 mentions) Pa1 183, by Thermo Fisher Scientific (1 mentions)

3 protocols using hrp conjugated goat anti rabbit igg sc 2054

1

Western Blot Analysis of BCL11B, Tax, and HBZ

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The western blot analyses were carried out as described previously.24 (link) The primary antibodies used at the indicated dilutions were anti-BCL11B (1/1000),16 (link) anti-BCL11B (1/1000) (Cell Signaling Technology, Beverly, MA, USA), anti-Tax (1/2000) (Taxy7),25 (link) anti-Tax2,26 (link) anti-α-tubulin (DM1A) (1/1000) (Calbiochem, San Diego, CA, USA) and α-HBZ (1/1000).9 (link) Horseradish peroxidase (HRP)-conjugated rat anti-mouse IgG kappa light chain (BD Biosciences, San Jose, CA, USA) and HRP-conjugated goat anti-rabbit IgG (sc-2054; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used as secondary antibodies (1/10 000). Immunoreactive bands were visualized with the Enhanced Chemiluminescence Detection System (Amersham Pharmacia Biotech, Orsay, France).
Takachi T., Takahashi M., Takahashi-Yoshita M., Higuchi M., Obata M., Mishima Y., Okuda S., Tanaka Y., Matsuoka M., Saitoh A., Green P.L, & Fujii M. (2015). Human T-cell leukemia virus type 1 Tax oncoprotein represses the expression of the BCL11B tumor suppressor in T-cells. Cancer Science, 106(4), 461-465.
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2

Western Blot Analysis of HTLV-1 Proteins

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The western blot analyses were carried out as described previously.(24 (link)) The primary antibodies used at the indicated dilutions were anti-BCL11B (1/1000),(16 (link)) anti-BCL11B (1/1000) (Cell Signaling Technology, Beverly, MA, USA), anti-Tax (1/2000) (Taxy7),(25 (link)) anti-Tax2,(26 (link)) anti α-tubulin (DM1A) (1/1000) (Calbiochem, San Diego, CA, USA) and α-HBZ (1/1000).(9 (link)) Horseradish peroxidase (HRP)-conjugated rat anti-mouse IgG kappa light chain (BD Biosciences, San Jose, CA, USA) and HRP-conjugated goat anti-rabbit IgG (sc-2054; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used as secondary antibodies (1/10 000). Immunoreactive bands were visualized with the Enhanced Chemiluminescence Detection System (Amersham Pharmacia Biotech, Orsay, France).
Takachi T., Takahashi M., Takahashi-Yoshita M., Higuchi M., Obata M., Mishima Y., Okuda S., Tanaka Y., Matsuoka M., Saitoh A., Green P.L, & Fujii M. (2015). Human T-cell leukemia virus type 1 Tax oncoprotein represses the expression of the BCL11B tumor suppressor in T-cells. Cancer Science, 106(4), 461-465.
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3

Immunoblotting of β-catenin and Vimentin

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A mouse monoclonal antibody of β-catenin (sc-7963), rabbit polyclonal antibody of vimentin (sc-5565), HRP conjugated goat anti-rabbit IgG (sc-2054), and HRP conjugated goat anti-mouse IgG (sc-2055) were from Santa Cruz Biotech. A rabbit polyclonal antibody of β-actin (PA1183) and Texas Red-X conjugated goat anti-mouse IgG (T6390) were from Thermo Scientific.
Lichtenberger L.M., Fang D., Bick R.J., Poindexter B.J., Phan T., Bergeron A.L., Pradhan S., Dial E.J, & Vijayan K.V. (2016). Unlocking aspirin’s chemopreventive activity: Role of irreversibly inhibiting platelet cyclooxygenase-1. Cancer prevention research (Philadelphia, Pa.), 10(2), 142-152.
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