Ps473

Manufactured by Cell Signaling Technology

Sourced in United States

PS473 is a laboratory instrument designed for the detection and quantification of phosphorylated proteins. It utilizes fluorescence-based detection methods to measure the levels of specific phosphorylated residues within a sample.

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Lab products found in correlation

Pan akt, by Cell Signaling Technology (1 mentions) Pfoxo1 3a, by Cell Signaling Technology (1 mentions) Alexa fluor 647 coupled antibody against γh2a x protein, by BD (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Hyperfilm ecl film, by GE Healthcare (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)

2 protocols using ps473

Akt Inhibitor MK-2206 Protocol

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The MK-2206 Akt-inhibitor was purchased from Selleckchem (Houston, TX, USA). The mouse monoclonal ß-Actin antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA). panAkt, pS473, pT308, panMERIT40, pMERIT40, pFOXO1/3a, panFOXO1, eGFP and ß-Actin antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Alexa Fluor 647-coupled antibody against γH2A.X protein was obtained from Becton Dickinson (Heidelberg, Germany). Hoechst33342 was purchased from Invitrogen (Eugene, OR, USA). DAKO Fluorescent mounting medium from Dako North America Inc. (Carpinteria, CA, USA) was used. All other chemicals were acquired from Sigma-Aldrich (St. Louis, MO, USA).

Szymonowicz K., Oeck S., Krysztofiak A., van der Linden J., Iliakis G, & Jendrossek V. (2018). Restraining Akt1 Phosphorylation Attenuates the Repair of Radiation-Induced DNA Double-Strand Breaks and Reduces the Survival of Irradiated Cancer Cells. International Journal of Molecular Sciences, 19(8), 2233.

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Protein Detection via Chemiluminescence

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Protein detection was performed using the SuperSignal West Pico Chemiluminescent substrate (Thermo Scientific) and Hyperfilm ECL films (GE Healthcare). Antibodies against Akt and phospho-Akt (pS473) were purchased from Cell Signaling Technology.

Kern D., Regl G., Hofbauer S.W., Altenhofer P., Achatz G., Dlugosz A., Schnidar H., Greil R., Hartmann T.N, & Aberger F. (2015). Hedgehog/GLI and PI3K signaling in the initiation and maintenance of chronic lymphocytic leukemia. Oncogene, 34(42), 5341-5351.

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