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Anti nedd4 2

Manufactured by Cell Signaling Technology
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Anti-Nedd4-2 is a primary antibody that specifically recognizes the Nedd4-2 protein. Nedd4-2 is an E3 ubiquitin-protein ligase that plays a role in the regulation of ion channels and transporters. The Anti-Nedd4-2 antibody can be used to detect and study the Nedd4-2 protein in various experimental applications.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Tumor necrosis factor α, by R&D Systems (1 mentions) Interleukin il 1β, by R&D Systems (1 mentions) Myeloperoxidase mpo, by R&D Systems (1 mentions) Ly294002, by MedChemExpress (1 mentions) Rosuvastatin, by MedChemExpress (1 mentions) Anti cox 4, by Cell Signaling Technology (1 mentions) Anti xbp1, by Abcam (1 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) Salubrinal, by Merck Group (1 mentions) Anti atf4, by Cell Signaling Technology (1 mentions) Anti phospho eif2α, by Cell Signaling Technology (1 mentions) Anti psd95, by Cell Signaling Technology (1 mentions) Anti map2, by Cell Signaling Technology (1 mentions) Anti pdi, by Cell Signaling Technology (1 mentions) Anti synapsin 1, by Abcam (1 mentions) Kainic acid, by Cayman Chemical (1 mentions) Anti ire1α, by Cell Signaling Technology (1 mentions) Anti atf6, by Abcam (1 mentions)

Close spelling variants of this product

NEDD4 (7 citations) Nedd4-2 (3 citations) Anti-NEDD4 (3 citations)

3 protocols using anti nedd4 2

1

Rosuvastatin Regulates Epithelial Sodium Channel

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Rosuvastatin, LY294002 was purchased from MedChemExpress (MJ, USA), and LPS was obtained from Sigma (MO, USA). Tumor necrosis factor (TNF)-α, myeloperoxidase (MPO), interleukin (IL)-1β and IL-10 enzyme-linked immunosorbent assay (ELISA) kits were procured from R&D Systems (MN, USA). MK-2206 was purchased from Target Molecule Corp (BSN, USA). Anti-ENaC α, β, and γ antibodies were procured from Biorbyt (CS, UK). Anti-Na,K-ATPase α1 and β1 antibodies were from Abcam (MA, USA). Anti-total Akt (T-Akt), anti-phosphorylated Akt (P-Akt), and anti-Nedd4-2 antibodies were from CellSignalling Technology (MA, USA).
Xu H.R., Yang Q., Xiang S.Y., Zhang P.H., Ye Y., Chen Y., Xu K.W., Ren X.Y., Mei H.X., Shen C.X., Ma H.Y., Smith F.G., Jin S.W, & Wang Q. (2021). Rosuvastatin Enhances Alveolar Fluid Clearance in Lipopolysaccharide-Induced Acute Lung Injury by Activating the Expression of Sodium Channel and Na,K-ATPase via the PI3K/AKT/Nedd4-2 Pathway. Journal of Inflammation Research, 14, 1537-1549.
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2

Neuronal ER Stress Modulation Assay

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Bovine serum albumin (BSA) was from Fisher Scientific (catalog: BP9706-100). Dimethyl sulfoxide (DMSO) was from Fisher Scientific (catalog: BP231-100). Thapsigargin was from Adipogen (catalog: AG-CN2-0003). Salubrinal was from Sigma Aldrich (catalog: SML0951). Saline was from Hanna Pharmaceutical (catalog: NC9054335). Kainic acid was from Cayman Chemical Company (catalog: 78050). The antibodies used in this study were purchased from ProteinTech (anti-Gapdh, RRID: AB_2107436), AbClonal (anti-XBP1 [RRID: AB_2757016] and anti-ATF6 [RRID: AB_2801582]), Abcam (anti-Synapsin I [RRID: AB_2200097], anti-PSD-95 [RRID: AB_303248], and anti-Map2 [RRID: AB_2138147]) and Cell Signaling (anti-Nedd4-2 [RRID: AB_1904063], anti-eIF2α [RRID: AB_10692650], anti-phospho-eIF2α [RRID: AB_2096481], anti-PDI [RRID: AB_2156433], anti-COX IV [RRID: AB_2797784], anti-IRE1α [RRID: AB_823545], and anti-ATF4 [RRID: AB_2616025]).
Lodes D.E., Zhu J, & Tsai N.P. (2021). E3 Ubiquitin Ligase Nedd4-2 Exerts Neuroprotective Effects During Endoplasmic Reticulum Stress. Journal of neurochemistry, 160(6), 613-624.
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3

Investigating Protein Regulation in HEK293 Cells

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Dimethyl sulfoxide (DMSO) was from Thermo Fisher Scientific (catalog: BP231–100). Tetrodotoxin citrate (TTX) was from Cayman Chemicals (catalog: 14964). Ionomycin was from AdipoGen (catalog: AG-CN2–0416-M001). The antibodies used in this study were purchased from Cell Signaling (anti-Nedd4–2, RRID: AB_1904063; anti-pan-14-3-3, RRID: AB_10860606; anti-Myc, RRID: AB_490778; anti-N-Cadherin, RRID: AB_2798427; anti-HA, RRID: AB_10691311, and anti-Ub, RRID: AB_331292), Millipore (anti-GluA1-N-terminus, RRID: AB_1977459), Santa Cruz Biotechnology (anti-α-tubulin, RRID: AB_628411), and Proteintech (anti-Gapdh, RRID: AB_2107436), and ABclonal (anti-PPP3CA, RRID: AB_2758155). Human embryonic kidney (HEK 293) cells were from ATCC (ATCC #CRL-1573, RRID: CVCL_0045). HEK 293 cell line is not listed as a commonly misidentified cell line by the International Cell Line Authentication Committee (ICLAC) and was not authenticated in this study. Cells were cultured in Dulbecco’s Modified Eagle Medium (Sigma, catalog: 10017CV) with 10% Fetal Bovine Serum (JM Bioscience, catalog: 100–500). Cells were used between passages 4 to 25 and kept at 37°C in a humidified incubator containing 5% CO2.
Zhu J., Lee K.Y., Jong T.T, & Tsai N.P. (2019). C2-lacking isoform of Nedd4-2 regulates excitatory synaptic strength through GluA1 ubiquitination-independent mechanisms. Journal of neurochemistry, 151(3), 289-300.
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