Rabbit anti fgf2 antibody

For FGF2 immunostaining, brain sections were prepared as described ^{34 (link)}. The brains of 12 NF-α1-KO mice and 12 WT littermates at 6-, 14-, and 30-wk of age (2 male and 2 female mice of each genotype, sex, and age) were dissected from perfused mice and embedded (24 mouse brains per block) in a gelatin matrix using MultiBrain™ Technology (NeuroScience Associates, Knoxville, TN). The MultiBrain™ block was sectioned in the coronal plane at 35 μm on an AO 860 sliding microtome. Every 8^th free-floating section was used for FGF2 immunohistochemistry and was stained as described ^{34 (link)}, using a rabbit anti-FGF2 antibody (1:1,500 dilution, LifeSpan Biosciences). For DCX staining, 35 μm brain sections from perfused FGF2 treated or naïve WT and CPE-KO mice were cut under contract by NeuroScience Associates. The sections were then stained for DCX using polyclonal rabbit anti-doublecortin (1:2,000, Cell Signaling, Danvers, MA) followed by Alexa Fluor 594 goat anti-rabbit secondary antibody (Invitrogen, Carlsbad, CA). Every 10^th free-floating section was used for DCX immunostaining.