The miR-18a mimic, inhibitor, and negative control miRNA were purchased from RiboBio (Guangzhou China) and transfected into cells at 100 nM concentrations via Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. KLF4 plasmid (1ug; Origene, Rockville, MD, USA) was also transfected into cells in the presence or absence of the miR-18a mimic by Lipofectamine 2000. After transfection twice in 48 h, cells were used in the subsequent experiments. RT-PCR and Western blotting were used to evaluate the transfection efficacy.
Mir 18a mimic
Manufactured by OriGene
Sourced in United States, China
The MiR-18a mimic is a synthetic double-stranded RNA molecule designed to mimic the function of the endogenous microRNA miR-18a. MicroRNAs are small, non-coding RNA molecules that play a role in the regulation of gene expression. The MiR-18a mimic can be used in laboratory research settings to study the biological functions of miR-18a.
Automatically generated - may contain errors
2 protocols using mir 18a mimic
1
Transfection Protocol for miR-18a Mimic and KLF4 Plasmid
2
Oligonucleotide Transfection with Lipofectamine
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Lipofectamine 2000 (Invitrogen) was used for oligonucleotide transfection in this study per the manufacturer’s recommendations. The miR-18a mimic, miR-18a inhibitor, and negative control (NC) miRNA were designed and synthesized by RiboBio (Guangzhou, P.R. China). IRF2 or CBX7 expression constructs (1 μg; OriGene, Beijing, P.R. China) were transfected into cells with or without miR-18a mimic. After transfection for 48 h, the cells were collected and analyzed by quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot to evaluate the transfection efficacy.
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