Total protein was isolated from macrophages were treated with EOC-derived exosomes (100 μg/ml) or miR-222-3p mimics, inhibitors and the miR-negative control for 96 or 48 hours. Antibodies against Mannose Receptor (Abcam, MA, USA), SOCS3 (Abcam, MA, USA), phospho-STAT3 (Tyr705) (Cell Signaling, MA, USA), and STAT3 (Cell Signaling, MA, USA) were used.
Mannose receptor
Manufactured by Abcam
Sourced in United States
The Mannose Receptor is a type I transmembrane glycoprotein that binds to mannose, N-acetylglucosamine, and fucose residues. It is primarily expressed on the surface of macrophages and dendritic cells, and plays a role in the innate immune response by facilitating the endocytosis and phagocytosis of pathogens and other foreign particles.
Automatically generated - may contain errors
Lab products found in correlation
Phospho stat3 tyr705, by Cell Signaling Technology (1 mentions)
Stat3, by Cell Signaling Technology (1 mentions)
Socs3, by Abcam (1 mentions)
Appropriate fluorescence conjugated secondary antibodies, by Jackson ImmunoResearch (1 mentions)
Ox 42, by Abcam (1 mentions)
Magnafire sp 2.1b software, by Olympus (1 mentions)
Bx51 microscope, by Olympus (1 mentions)
Porcine serum, by Thermo Fisher Scientific (1 mentions)
Prolong gold antifade mountant, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 546 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Nucblue fixed cell stain, by Thermo Fisher Scientific (1 mentions)
Ab150686, by Abcam (1 mentions)
Hrp dab detection ihc kit, by Abcam (1 mentions)
Pannoramic midi, by 3DHISTECH (1 mentions)
4 protocols using mannose receptor
1
Macrophage Exosome Response
2
Microglial response in intracerebral hemorrhage
3
Immunofluorescence Staining of Macrophages
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The CD14+ cells (1 × 106) were cultured on slides for 7 days at 37°C in a humidified atmosphere containing 5% CO2 to obtain MDM, and the cells were washed in PBS and fixed in 2% formaldehyde for 15 min. To avoid unspecific binding, the MDM were blocked for 30 min with 2% porcine serum (Gibco) in PBS (PBS-PS). Posteriorly, the cells were incubated 1 h with primary mAbs to Mannose Receptor (4 μg/mL, Abcam) TCRαβ (4 μg/mL, Thermo Fischer) and CD3 (1:100 cell signaling). The following were used as secondary mAbs (diluted in PBS-PS): donkey anti-rabbit IgG Alexa Fluor-488 (1:100) and donkey anti-rat IgG Alexa Fluor-647 (1:100) provided by Jackson ImmunoReseach and Goat anti-Mouse IgG Alexa Fluor-546 provided by Invitrogen, cells were incubated 1 h. Finally, the samples were washed and incubated with 4_,6-diamidino-2 phenylindole dihydrochloride (DAPI; NucBlue Fixed Cell Stain, Molecular Probes) for 10 min for nuclei labeling and mounting with ProLong Gold Antifade Mountant (Invitrogen). The slides were examined by confocal microscopy FV-1,000 Olympus, and FIJI software was used for the analysis.
4
Histopathological Analysis of Skin Tissue
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Removed skin tissues including incisions were immediately placed in 10% formalin solution. The fixed tissues were dehydrated using a series of ethanol solutions, and the dehydrated tissues were then embedded in paraffin for block preparation. A constant thickness of slide was sectioned (3 µm). As a control, the same thickness of paraffin slide using normal skin tissue was made. The tissue sections were treated with H&E and MT staining (Abcam: ab150686, Cambridge, UK). For immunostaining, deparaffinized tissue sections were treated using a microwave antigen-retrieval procedure with 0.01 M sodium citrate buffer. After blocking, the sections incubated with antibodies against iNOS (N-20, Santa Cruz Biochemical, Dallas, TX, USA) and mannose receptor (Abcam). For visualization, an HRP/DAB detection IHC kit (Abcam) was used according to the manufacturer’s instructions. These sections were observed using a slide scanner (Pannoramic MIDI; 3DHISTECH Ltd., Budapest, Hungary) and a panoramic viewer (Version 1.15.3; Pannoramic MIDI; 3DHISTECH Ltd., Budapest, Hungary) program.
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