Cyclophosphamide

A myeloablative protocol containing 30 mg/m² per day fludarabine (i.v. once daily, day −13 to day −9) (Guangdong South of the Five Ridges, Guangzhou, China) and 2 g/m² per day cytarabine (i.v. once daily, day −13 to day −9) (Pfizer), followed by 1.8 g/m² per day cyclophosphamide (i.v. once daily, day −8 to day −7) (Baxter, Halle, Germany) as well as 0.8 mg/kg busulfan (i.v. four times daily, day −6 to day −4) (Kyowa Hakko Kylin, Beijing, China) (FA5-BUCY) was used to condition patients with acute leukaemia.
A non-myeloablative protocol containing 30 mg/m² per day fludarabine (i.v. once daily, day −5 to day −2) (Guangdong South of the Five Ridges) and 30 mg/kg per day cyclophosphamide (i.v. once daily, day –5 to day −2) (Baxter) and 10 mg/kg ATG (Sanofi-Aventis; 2.5 mg/kg i.v. once daily from day −4 to day −1) (FC protocol) was used to condition patients with aplastic anaemia.

Cyclophosphamide was obtained from Pfizer International (NY, USA). Lactate dehydrogenase (LDH), total protein, cholesterol, and triacylglycerol kits were products of Randox Laboratories Limited (Admore, Crumlin, Co-Antrim, UK). Griess reagent kit was purchased from Cayman Chemical (Ann Arbor, Michigan, USA). Sodium nitroprusside (C₅FeN₆Na₂O), hydrogen peroxide (H₂O₂), butylated hydroxytoluene (BHT), vitamin C, 2- thiobarbituric acid (2, 6-dihydroxypyrimidine-2-thiol; TBA), dipotassium hydrogen phosphate (K₂HPO₄), potassium dihydrogen phosphate (KH₂PO₄), potassium chloride (KCl), reduced glutathione (GSH), oxidized glutathione (GSSG), 1-chloro-2, 4-dinitrobenzene (CDNB), Ellman’s reagent DTNB [5,5ˈ-dithiobis(2-nitrobenzoic acid)], pyrogallol, reduced nicotinamide adenine dinucleotide phosphate (NADPH), and xylenol tetrasodium (C₃₁H₂₈N₂Na₄O₁₃S), chloroform, isopropanol, triton X-100, ethanol, ammonium ferrothiocyanate, copper II nitrate (Cu(NO₃)₂.3H₂O), triethanolamine, oxalic acid bis(cyclohexylidenehydrazide), and ammonium hydroxide (NH₄OH) were procured from Aldrich Sigma Chemical Company (St. Louis, MO, USA). Iron (II) sulfate heptahydrate (FeSO₄.7H₂O) was imported from Merck KGaA, Darmstadt, Germany. Sulfuric acid was supplied by Ajax Finechem, Australia. All other chemicals and reagents were of analytical grade and were procured from British Drug Houses (Poole, UK).