40 μm pore size cell strainer

Manufactured by BD

Sourced in United States

The 40-μm-pore-size cell strainer is a laboratory equipment designed to filter and separate cells based on size. It features a mesh with pores measuring 40 microns in diameter, allowing for the efficient isolation and purification of cell samples.

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Lab products found in correlation

Rnalater solution, by Merck Group (2 mentions) Betaine, by Merck Group (2 mentions) Rnalater solution, by Thermo Fisher Scientific (2 mentions) Tween 20, by Merck Group (2 mentions) Balb c mice, by Jackson ImmunoResearch (1 mentions) Red blood cell lysis buffer, by BioLegend (1 mentions) Addavax adjuvant, by InvivoGen (1 mentions) Acid citrate dextrose, by Merck Group (1 mentions) Ack lysing buffer, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Cell strainer (717 citations) 40-μm cell strainer (575 citations) 40-μm strainer (62 citations) 40-μm pore cell strainer (7 citations) Pore size cell strainers (2 citations)

7 protocols using 40 μm pore size cell strainer

Isolation of Gut Microbiome Fractions

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A total of 30 fifth-instar larvae for each gut region, foregut and hindgut were dissected with sterile forceps and scissors in a sterile clean bench. Following dissection, the gut tissues were immediately submerged in 10 ml of RNAlater solution (Invitrogen, Vilnius, Lithuania). Tissues submerged in RNAlater solution were mixed with 2 ml of 6% (w/v) betaine (Sigma Aldrich, St.
Louis, MO, USA) and placed on ice prior to crushing with mortar and pestle until gut homogenates were formed. Thereafter, fluorescent E. mundtii were separated from the intestinal debris by filtration through 40 μm pore-size cell strainers (Falcon, NY, USA). The filtrates were then separated into aliquots of 600 µl each and kept at -80˚C for the sorting experiment.

Mazumdar T., Teh B.S., Murali A., Schmidt-Heck W., Schlenker Y., Vogel H, & Boland W. (2021). Transcriptomics Reveal the Survival Strategies of Enterococcus mundtii in the Gut of Spodoptera littoralis. Journal of chemical ecology, 47(2).

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Isolation of Gut Microbiome Fractions

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Mazumdar T., Teh B.S., Murali A., Schmidt‐Heck W., Schlenker Y., Vogel H., & Boland W. (2020). Survival strategies of Enterococcus mundtii in the gut of Spodoptera littoralis: a live report.

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Antigen-Specific Immune Response Study

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Six-week-old female BALB/c mice were purchased from The Jackson Laboratory. The mice were housed in ventilated cages in environmentally controlled rooms at the Scripps Research Institute (TSRI), in compliance with an approved IACUC protocol and AAALAC guidelines. At week 0, each mouse was immunized with 100 μl (50 μg) of antigen formulated in 50 μl AddaVax adjuvant (InvivoGen) per the instructions of the manufacturer via the subcutaneous route. At week 3 and week 6, the animals were boosted with 10 μg of antigen formulated in AddaVax adjuvant. At week 8, the animals were terminally bled by cardiac puncture and the anticoagulant acid citrate dextrose (Sigma-Aldrich) was added to the samples at a 1:10 ratio. Samples were spun at 1,000 rpm for 10 min at 4°C to separate plasma and cells. Red blood cell lysis buffer (BioLegend) was added to the cell fraction. After 2 rounds of washing with PBS, peripheral blood mononuclear cells (PBMCs) were resuspended in Bambanker freezing media (Lymphotec Inc.). Spleens were also harvested and ground against a 40-μm-pore-size cell strainer (BD Falcon) to release the splenocytes into a cell suspension. The cells were centrifuged, treated with 10 ml of red blood cell (RBC) lysis buffer per manufacturer specifications, and resuspended in Bambanker freezing media for cell freezing.

Morris C.D., Azadnia P., de Val N., Vora N., Honda A., Giang E., Saye-Francisco K., Cheng Y., Lin X., Mann C.J., Tang J., Sok D., Burton D.R., Law M., Ward A.B., He L, & Zhu J. (2017). Differential Antibody Responses to Conserved HIV-1 Neutralizing Epitopes in the Context of Multivalent Scaffolds and Native-Like gp140 Trimers. mBio, 8(1), e00036-17.

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Isolation of Mouse Splenic Cells

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Following euthanasia, spleens from naive mice were aseptically removed and transferred into individual tubes containing 5 ml of RPMI 1640 medium. The organ was macerated by pressing the spleens through a 40-μm pore-size cell strainer (BD Falcon, Franklin Lakes, NJ, USA) with the aid of a sterile syringe plunger. Cells were centrifuged at 300×g for 5 min at 4 °C, the supernatant was discarded and the red blood cells were lysed by ACK Lysing Buffer (Gibco Invitrogen). After further washings, the cells were resuspended in complete medium, diluted in Turk’s solution, counted in a Neubauer’s chamber and used as described below.

Barros M.S., Lara P.G., Fonseca M.T., Moretti E.H., Filgueiras L.R., Martins J.O., Capurro M.L., Steiner A.A, & Sá-Nunes A. (2019). Aedes aegypti saliva impairs M1-associated proinflammatory phenotype without promoting or affecting M2 polarization of murine macrophages. Parasites & Vectors, 12, 239.

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Aspergillus fumigatus Strain Generation

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The A1163 Δku80^{64 (link)} and B-5233^{65 (link)} strains of A. fumigatus were used as wild-type strains. The ΔrodA, ΔpksP, and ΔrodA/pksP deletion mutants^{43 (link),47 (link)} and the mutant strains in the DHN–melanin biosynthetic pathway (ΔpksP, Δayg1, Δabr1, Δabr2, Δarp1, and Δarp2) in the B-5233 background^{66 (link)} were generated previously. Wild-type CBS110.46 and CBS386.75 strains with albino conidia^{38 (link)} were also used, as indicated. All strains were grown on 2% malt extract agar or YAG agar for 7 days at 28 °C. The conidia were harvested from agar slants using phosphate buffer saline (PBS) (Gibco, Thermo Fisher Scientific) with 0.05% Tween 20 (Sigma-Aldrich), followed by gentle agitation and subsequent filtration through a 40-μm-pore size cell strainer (Falcon). The concentration of conidia/mL was determined by counting in a Neubauer chamber. Swollen conidia and germ tubes were obtained after incubation in Sabouraud liquid culture medium at 37 °C for 4 h and 6 h, respectively. Heat inactivation of conidia was performed by incubation for 30 min at 90 °C, whereas UV inactivation was performed by exposing conidia to UV light for 3 h.

Gonçalves S.M., Duarte-Oliveira C., Campos C.F., Aimanianda V., ter Horst R., Leite L., Mercier T., Pereira P., Fernández-García M., Antunes D., Rodrigues C.S., Barbosa-Matos C., Gaifem J., Mesquita I., Marques A., Osório N.S., Torrado E., Rodrigues F., Costa S., Joosten L.A., Lagrou K., Maertens J., Lacerda J.F., Campos A J.r., Brown G.D., Brakhage A.A., Barbas C., Silvestre R., van de Veerdonk F.L., Chamilos G., Netea M.G., Latgé J.P., Cunha C, & Carvalho A. (2020). Phagosomal removal of fungal melanin reprograms macrophage metabolism to promote antifungal immunity. Nature Communications, 11, 2282.

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Aspergillus fumigatus Conidia Harvesting

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A. fumigatus was grown on 2% malt extract agar for 7 days at 28°C. The conidia were harvested from agar plates using phosphate buffer saline (PBS) (Gibco) with 0.05% Tween 20 (Sigma-Aldrich), followed by gentle agitation and subsequent filtration through a 40-μm-pore size cell strainer (Falcon). The concentration of conidia/mL was determined by counting in a Neubauer chamber.

Antunes D., Gonçalves S.M., Matzaraki V., Rodrigues C.S., Gonçales R.A., Rocha J., Sáiz J., Marques A., Torrado E., Silvestre R., Rodrigues F., van de Veerdonk F.L., Barbas C., Netea M.G., Kumar V., Cunha C, & Carvalho A. (2022). Glutamine Metabolism Supports the Functional Activity of Immune Cells against Aspergillus fumigatus. Microbiology Spectrum, 11(1), e02256-22.

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Bronchoalveolar Lavage Fluid Collection

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Prior to each bronchoscopy, the bronchoscope was washed using Enzol enzymatic detergent (Johnson and Johnson Medical, New Brunswick, NJ), rinsed with water, and placed in a sterilizing bath of 2.5 % glutaraldehyde solution for at least 15 min. The bronchoscope was passed through the laryngeal folds and directed into the right main stem bronchus without suction. Once wedged, four aliquots of 10 ml sterile saline were lavaged. Bronchoalveolar lavage (BAL) fluid was immediately placed on ice and transported to the laboratory for processing. Unfractionated BAL fluid was filtered through a 40-μm-pore-size cell strainer (BD Falcon, Franklin Lakes, NJ). Lung cells were pelleted (5 min at 500g), and supernatant fluid was collected and stored at −80 °C until use.

Morris A., Paulson J.N., Talukder H., Tipton L., Kling H., Cui L., Fitch A., Pop M., Norris K.A, & Ghedin E. (2016). Longitudinal analysis of the lung microbiota of cynomolgous macaques during long-term SHIV infection. Microbiome, 4, 38.

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