Ultrospec 2100 spectrophotometer

Manufactured by Cytiva

Sourced in United States

The UltroSpec 2100 is a spectrophotometer that measures the absorbance of light passing through a sample. It is designed to analyze the chemical composition of various substances by detecting the amount of light absorbed at specific wavelengths.

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Lab products found in correlation

Talos l120c microscope, by Thermo Fisher Scientific (1 mentions) Primerscript rt reagent kit, by Takara Bio (1 mentions) Easypure rna kit, by Transgene (1 mentions) Applied biosystems 7500 fast real time pcr system, by Thermo Fisher Scientific (1 mentions) Qiaprep spin miniprep kit, by Qiagen (1 mentions) Nanodrop one, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Ultrospec 2100 Pro spectrophotometer (30 citations) Ultrospec 2100 (14 citations) Ultrospec 2100 pro UV-Vis spectrophotometer (13 citations) Ultrospec 2100 pro UV/Visible Spectrophotometer (5 citations)

3 protocols using ultrospec 2100 spectrophotometer

Characterization of Nanodrug Formulations

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Visible spectra of the nanodrugs were recorded using an UltroSpec 2100 spectrophotometer (Amersham Biosciences) and a 1-cm-path-length quartz cuvette. The concentration of Nps was calculated by interpolating the A₅₃₀ nm values on a calibration curve obtained using uncoated nanogold in 5 mM sodium citrate buffer, pH 6.0 (stock solution: 3.3 × 10¹¹ Nps/ml; A₅₃₀ nm: 0.96 U/ml).
Dynamic light scattering (DLS) measurements were performed using a DLS DYNAPRO 99 instrument (Wyatt) operating with the laser intensity set to 20% power. The nanodrugs were diluted 1:10 in 5 mM sodium citrate buffer, pH 6.0 (1–3 × 10¹⁰ Nps/ml) and analyzed using 25–30 independent measurements of 10-s duration at 20 °C. The calculation of the hydrodynamic radius of the Nps was performed using DLS regularization analysis.
Transmission electron microscopy (TEM) analysis were performed using a TALOS L120C microscope (ThermoScientific) as described previously [23 (link)]. Morphometric analysis of nanoparticles shape and diameter was performed using the ImageJ software, essentially as previously reported [30 (link)].

Corti A., Sacchi A., Gasparri A.M., Monieri M., Anderluzzi G., Colombo B., Gori A., Mondino A, & Curnis F. (2021). Enhancement of doxorubicin anti-cancer activity by vascular targeting using IsoDGR/cytokine-coated nanogold. Journal of Nanobiotechnology, 19, 128.

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Quantitative Analysis of Stress Response Genes

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Total RNA was extracted with an EasyPure RNA Kit (TransGen Biotech, Beijing, China), according to the manufacturer’s protocol. The quality and quantity of the extracted RNA was measured using an Ultrospec 2100 spectrophotometer (Amersham Biosciences, Pittsburgh PA, USA) at 260 nm. cDNA synthesis was performed with a PrimerScript RT reagent Kit (TaKaRa, Dalian, China) according to the manufacturer’s instruction by using a 6-bp random primers set. Selected fragments of crdR, crdA, crdS, and crdC, which were amplified with primers qcrdR-1 & qcrdR-2, qcrdA-1 & qcrdA-2, qcrdS-1 & qcrdS-2, and qcrdC-1 & qcrdC-2 (Table 2), were ligated into pMD18-T vectors respectively. Then, using those constructs as standard copies, q-RT-PCR quantification was performed using an Applied Biosystems 7500 fast realtime PCR system (Applied Biosystems, Grand Island, NY ) with SYBR Premix E*TaqII (TaKaRa). All samples were run in triplicate [39 (link)].

Yu X., Zhang C., Yang L., Zhao L., Lin C., Liu Z, & Mao Z. (2015). CrdR function in a curdlan-producing Agrobacterium sp. ATCC31749 strain. BMC Microbiology, 15(1), 25.

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Bacterial Growth and Plasmid Quantification

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Cells were cultured in 100 mL LB supplemented with additional 10 g/L glucose and 50 μg/mL kanamycin in a 300 mL Erlenmeyer flask. Flasks were inoculated from overnight cultures to an OD of 0.1. The cultures were then sampled every 1–2 h by measuring OD as well as collecting 2 mL of culture in a microcentrifuge tube, followed by centrifugation, and transferring 800 μL of cell‐free supernatant to a fresh sample tube. Acetate concentration in the supernatant was measured using the Cedex Acetate V2 Bio HT kit on a Cedex HT system (Roche Custom Biotech). OD₆₀₀ was measured using a Ultrospec 2100 Spectrophotometer (Amersham Biosciences).
For plasmid concentration measurements, cells were inoculated from an overnight culture to an OD₆₀₀ of 0.1 in 10 mL LB with appropriate antibiotics and additional 10 g/L glucose in a 100 mL Erlenmeyer flask at 37°C and 220 rpm overnight. A cell mass of an equivalent of a OD₆₀₀ 3 was used for plasmid DNA purification using a QIAprep® Spin Miniprep Kit (Qiagen). DNA was eluted in 50 μL elution buffer and concentration was measured using a NanoDrop one (Thermo Fisher Scientific).

Mund M., Weber W., Degreif D, & Schiklenk C. (2023). A MAD7‐based genome editing system for Escherichia coli. Microbial Biotechnology, 16(5), 1000-1010.

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