Anti bruchpilot

Manufactured by Developmental Studies Hybridoma Bank

Anti-Bruchpilot is a monoclonal antibody produced by the Developmental Studies Hybridoma Bank. It is designed to detect the Bruchpilot protein, which is a component of the active zone in synapses of Drosophila melanogaster.

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Lab products found in correlation

Cy3 conjugated anti hrp, by Jackson ImmunoResearch (1 mentions) Alexa fluor 647 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti chicken, by Abcam (1 mentions) Alexa fluor 568 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Chicken polyclonal anti gfp antibody, by Abcam (1 mentions) Anti rfp, by Thermo Fisher Scientific (1 mentions) Lsm 700, by Zeiss (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Alexa 568, by Thermo Fisher Scientific (1 mentions) Anti rab11, by Abcam (1 mentions) Anti rab5, by Abcam (1 mentions) Anti rab11, by BD (1 mentions) Lamp1, by Abcam (1 mentions) Anti gfp, by Developmental Studies Hybridoma Bank (1 mentions)

Close spelling variants of this product

Mouse anti-Bruchpilot (7 citations) Mouse anti-bruchpilot (NC82), (4 citations) Mouse anti-Bruchpilot antibody (2 citations) Mouse anti-Bruchpilot Brp (nc82, (2 citations)

4 protocols using anti bruchpilot

Neuromuscular Junction Analysis in Aging Flies

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For neuromuscular junction (NMJ) analysis, ventral abdominal body-wall muscle preparations were dissected in Ca²⁺ free saline from 3, 7, 15, 20 and 30-day old adult males [37 (link)]. For assessing Aβ synaptotoxicity, we selected the ventral abdominal NMJ in the third abdominal hemisegment (Fig 1A). Samples were fixed in 4% formaldehyde in PBS, and immunostained with monoclonal antibody nc82 (anti-Bruchpilot 1:20, Developmental Studies Hybridoma Bank) visualized with α-mouse Alexa-488 (1:500, Invitrogen) and with Cy3-conjugated anti-HRP (1:200, Jackson Immuno Research). Anti-HRP signal reveals neuronal membranes, delimitating the motor neuron terminals. Bruchpilot is a CAST (CD3E-associated protein) homolog localized to the presynaptic specialization [38 (link)], and thus that was used to reveal active zones in the motor neuron terminal. Each Aβ-expressing genotype was processed simultaneously with its respective age-matched controls, to control for quantitative differences due to culture conditions.

López-Arias B., Turiégano E., Monedero I., Canal I, & Torroja L. (2017). Presynaptic Aβ40 prevents synapse addition in the adult Drosophila neuromuscular junction. PLoS ONE, 12(5), e0177541.

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Visualizing Drosophila Lobula Anatomy

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We used 3–5 days old female flies to visualize LC anatomy (Fig S1B) and the distribution of presynaptic and postsynaptic sites in the lobula (Fig 2A). Primary antibodies were anti-Bruchpilot (mouse monoclonal antibody Nc82, supernatant, Developmental Studies Hybridoma Bank, 1:10 dilution), anti-DsRed rabbit polyclonal antibody (Takara, 632496, 1:200 dilution), and anti-GFP chicken polyclonal antibody (abcam, 13970, 1:1000 dilution). Secondary antibodies used were Alexa Fluor 488 goat anti-chicken (abcam, ab150169, 1:1000 dilution), Alexa Fluor 568 goat anti-rabbit (ThermoFisher Scientific, A-11036, 1:200 dilution), and Alexa Fluor 647 goat anti-mouse (ThermoFisher Scientific, A-21236, 1:200 dilution).

Städele C., Keleş M.F., Mongeau J.M, & Frye M.A. (2020). Non-canonical receptive field properties and neuromodulation of feature detecting neurons in flies. Current biology : CB, 30(13), 2508-2519.e6.

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Multimodal Neuroanatomical Profiling of Drosophila Larvae

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Larvae were dissected in cold phosphate-buffered saline (PBS), fixed in 4% formaldehyde in PBS, washed with PBST (0.1% Triton X-100), and incubated with primary antibodies overnight at 4°C. Antibodies used include anti-Bruchpilot (Developmental Studies Hybridoma Bank (DSHB), antibody nc82, used at 1:50), anti-HRP (Jackson Immunoresearch, 123-605-021, used at 1:1000), anti-RFP (ThermoFisher # R10367, used at 1:2000), anti-Repo (DSHB, used at 1:20), anti-Synaptotagmin [57 (link)], anti-discs-large (DLG) (DSHB, used at 1:2000), anti-Futsch (DSHB antibody 22C10, used at 1:20), and anti-HP1 (DSHB C1A9, used at 1:20). Affinity purified rabbit anti-Tmep was used at 1:20.

Cho T.S., Beigaitė E., Klein N.E., Sweeney S.T, & Bhattacharya M.R. (2022). The putative Drosophila TMEM184B ortholog Tmep ensures proper locomotion by restraining ectopic firing at the neuromuscular junction. Molecular neurobiology, 59(4), 2605-2619.

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Immunohistochemical Analysis of Olfactory Receptors

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The following primary mouse antibodies were used: anti-GFP (1:100), anti-Bruchpilot (1:50, nc82, supernatant, Developmental Studies Hybridoma Bank [DSHB] ), and anti Rab11 (1:100, BD Biosciences). The primary rabbit antibodies, anti-GFP (1:2,000, TP-401, Torrey Pines), Lamp1, anti-Rab5, anti-Rab11 (1:100, Abcam), anti-Or22a (1:10,000) and anti-Orco (1:10,000) were gifts from Richard Benton. The secondary antibodies were conjugated to Alexa 488 or Alexa 568 (1:500, Molecular Probes). Antenna immunohistochemistry was performed as described previously (Couto et al., ). Or22a images were captured from a subset of stereotypic Or22a sensilla opposite to the arista. The confocal microscopy images were collected on an LSM 700 (Zeiss) and analyzed on a Zen image browser.

Sanchez G.M., Alkhori L., Hatano E., Schultz S.W., Kuzhandaivel A., Jafari S., Granseth B, & Alenius M. (2016). Hedgehog Signaling Regulates the Ciliary Transport of Odorant Receptors in Drosophila. Cell reports, 14(3).

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