Anti rabbit igg

Manufactured by Bioss Antibodies

Sourced in Denmark, China

Anti-rabbit IgG is a laboratory reagent used to detect and quantify rabbit immunoglobulin G (IgG) in various immunoassays. It functions by specifically binding to rabbit IgG, facilitating the identification and measurement of rabbit IgG in samples.

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Lab products found in correlation

Cyclin d1, by Cell Signaling Technology (1 mentions) Fitc conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Hrp conjugated goat anti mouse igg, by Promega (1 mentions) Mouse monoclonal anti myc, by Santa Cruz Biotechnology (1 mentions) Fusion solo x, by Vilber (1 mentions) Ecl substrate, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Dojindo Laboratories (1 mentions) Can get signal a, by Toyobo (1 mentions) Anti mouse igg alexa flour 488, by Thermo Fisher Scientific (1 mentions) Block ace, by Sumitomo Pharma (1 mentions)

4 protocols using anti rabbit igg

PEDV Infection Dynamics in Vero Cells

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Vero cells (ATCC, CCL-33) were grown in Dulbecco Minimal Essential Medium (D-MEM) (Gibco BRL, MD, US) supplemented with 10% heat-inactivated fetal bovine serum (PAN), 1% penicillin/streptomycin (Invitrogen) at 37℃ in 5% CO₂ atmosphere incubator. In the present study, the CH/SXYL/2016 strain of PEDV (GenBank accession number is MF462814) was isolated from intestinal tract contents of PEDV infected piglets in Shaanxi province in China. 0.5 MOI PEDV (containing 10 μg/ml trypsin) infection solution was incubated for 1 h, then the virus was removed and washed 3 times with PBS, then DMEM containing 2% FBS was added, and cell samples were taken at the indicated time points for detection. Viral titers were determined by median tissue culture infectious dose(TCID₅₀) (Reed and Muench, 1938 ).
Antibodies against cdc2, cdk2, cdk4, cdk6, Cyclin B1, Cyclin D1, Phospho-p53 (Ser15 and Ser20), p21 Waf1/Cip1 and β-Actin were purchased from Cell Signaling Technology. Antibodies against Cyclin A, Cyclin E, p53 were purchased from Santa Cruz. Horseradish peroxidase (HRP)-conjugated anti-mouse IgG and anti-rabbit IgG were purchased from Bioss. FITC-conjugated antirabbit IgG was purchased from Molecular Probes.

Sun P., Wu H., Huang J., Xu Y., Yang F., Zhang Q, & Xu X. (2018). Porcine epidemic diarrhea virus through p53-dependent pathway causes cell cycle arrest in the G0/G1 phase. Virus Research, 253, 1-11.

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MERS-CoV Structural Protein Detection

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Transfected cells were lysed in Triton X-100 lysis buffer (1% Triton X-100, 50 mM Tris–Cl [pH 8.0], 150 mM NaCl, 1 mM EDTA) on ice and cleared by centrifugation at 1,000 × g for 10 min at 4 °C. The concentrated VLPs and transfected cell lysates were mixed with SDS solubilizer. Samples were heated at 95 °C for 10 min, separated in 8% or 15% (wt/vol) polyacrylamide-SDS gels, transferred to PVDF membranes, and probed with polyclonal mouse anti-MERS-CoV S (Sino Biological, 1:1000), polyclonal rabbit anti-MERS-CoV M (GeneTex, 1:1000), polyclonal rabbit anti-MERS-CoV E (GeneTex, 1:1000), or monoclonal mouse anti-myc (Santa Cruz, 1:1000) antibodies. The membranes were then probed with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Promega, 1:1000) or anti-rabbit IgG (Bioss, 1:1000) and incubated with ECL substrate (Thermo Fisher Scientific), and the signals were detected using a Fusion Solo X (Vilber). Band density on western blot membranes was analyzed using Evolution Capt software.

Park J.E., Kim J.H., Park J.Y., Jun S.H, & Shin H.J. (2022). A chimeric MERS-CoV virus-like particle vaccine protects mice against MERS-CoV challenge. Virology Journal, 19, 112.

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Dual Fluorescent Immunohistochemistry for NK-3R and CD68

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Double-fluorescent IHC for NK-3R-CD68 was performed using anti-NK-3R antibody (1:100) (#bs-0166R, anti-rabbit IgG, Bioss) and anti-CD68 (1:100) (#KP1, anti-mouse IgG, Dako, Glostrup, Denmark). The secondary antibodies applied were anti-rabbit IgG Alexa Flour 568 and anti-mouse IgG Alexa Flour 488 (Thermo Fisher, Tokyo, Japan). Antibodies were diluted with Can Get Signal A (TOYOBO, Osaka, Japan). After antigen retrieval, sections were treated with Block Ace (DS Pharma Bio-medical, Osaka, Japan) for 20 min at room temperature. Specimens were incubated with primary antibodies at 4 °C overnight and then incubated with secondary antibodies (1:200) for 1 h at room temperature. After the reaction, the specimens were stained with 1 mg/mL DAPI (Dojindo Laboratories, Kumamoto, Japan).

Yoshida S., Shimo T., Takabatake K., Murase Y., Obata K., Okui T., Kunisada Y., Ibaragi S., Nagatsuka H, & Sasaki A. (2021). Expression of Neurokinin B Receptor in the Gingival Squamous Cell Carcinoma Bone Microenvironment. Diagnostics, 11(6), 1044.

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Glioma Pathological Grading via ARSD Immunohistochemistry

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Paraffin sections from 12 glioma patients were obtained from surgically resected gliomas and classified by senior physicians in the department of pathology in our hospital according to WHO classification (WHO I: 3 samples, WHO II: 3 samples, WHO III: 3 samples, WHO IV: 3 samples). Paraffin sections were antigen-repaired using 1% sodium citrate (Bioss, Beijing, China), and incubated overnight with 0.6% ARSD antibody (SAB, China) at 4°C. The sections were incubated with Antirabbit IgG (Bioss, Beijing, China), and dripping with horseradish labeled chain enzyme ovalbumin working solution.

Song Z., Zhao Z., Zhu S., Jin Q., Zhang S., Wang Z., Shen B., Wang Z, & Zhao Z. (2023). Arylsulfatase D is a prognostic biomarker that promotes glioma cells progression through JAK2/STAT3 pathway and M2 macrophage infiltration. Frontiers in Oncology, 13, 1228426.

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