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Alexa fluor 488 goat anti rabbit immunoglobulin g h l

Manufactured by Thermo Fisher Scientific

Alexa Fluor 488 goat anti-rabbit immunoglobulin G (H+L) is a fluorescently labeled secondary antibody used to detect and visualize rabbit primary antibodies in various immunoassays and imaging applications. It is composed of goat-derived antibodies specific to the heavy and light chains of rabbit immunoglobulin G, conjugated to the Alexa Fluor 488 fluorescent dye.

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2 protocols using alexa fluor 488 goat anti rabbit immunoglobulin g h l

1

Antibody Detection for Protein Analysis

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An antibody against PKR (sc-707) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-p-PKR (pT451, 1120–1) and -p-eIF2α (S51) antibodies were obtained from Epitomics (Burlingame, CA), and an anti-paxillin antibody (catalog #2542) was obtained from Cell Signaling Technology (Beverly, MA). A mouse anti-β-actin antibody was obtained from Sigma-Aldrich (St. Louis, MO). The misfolded anti-PrP antibody AMF-1c-120 was provided by Amorfix Life Sciences Ltd. (Mississauga, ON, Canada). Rabbit monoclonal antibodies against native PrP (EP1802Y) and BAG3 (catalog #ab92309) were purchased from Abcam (Cambridge, UK). Alexa Fluor 488 goat anti-rabbit immunoglobulin G (H+L) was purchased from Life Technologies.
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2

Antibody identification and validation

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An antibody against PKR (sc-707) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-p-PKR (pT451, 1120-1) and -p-eIF2α (S51) antibodies were obtained from Epitomics (Burlingame, CA), and an anti-paxillin antibody (catalog #2542) was obtained from Cell Signaling Technology (Beverly, MA). A mouse anti-β-actin antibody was obtained from Sigma-Aldrich (St. Louis, MO). The misfolded anti-PrP antibody AMF-1c-120 was provided by Amorfix Life Sciences Ltd (Mississauga, ON, Canada). Rabbit monoclonal antibodies against native PrP (EP1802Y) and BAG3 (catalog #ab92309) were purchased from Abcam (Cambridge, UK). Alexa Fluor 488 goat anti-rabbit immunoglobulin G (H + L) was purchased from Life Technologies.
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