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Anti cd28 antibody 37.51

Manufactured by BioLegend

The Anti-CD28 antibody (37.51) is a lab equipment product used for research purposes. It is a monoclonal antibody that binds to the CD28 receptor, which is expressed on the surface of T cells and plays a role in T cell activation and co-stimulation.

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3 protocols using anti cd28 antibody 37.51

1

Nano-aAPC Preparation via MHC-Ig Conjugation

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Soluble MHC-Ig dimers, Kb-Ig, was prepared and loaded with peptides as described (17 (link)), see Supplemental Materials. Nano-aAPC were manufactured by direct conjugation of MHC-Ig dimer and anti-CD28 antibody (37.51; Biolegend) to MACS Microbeads (Miltenyi Biotec) as described previously (18 (link)).
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2

Nano-aAPC Production with MHC-Ig Dimers

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Soluble MHC-Ig dimers, Kb-Ig and Db-Ig, were prepared and loaded with peptides as described;8 (link) see Supporting Information. Nano-aAPC were manufactured by direct conjugation of MHC-Ig dimer and anti-CD28 antibody (37.51; BioLegend) to MACS Microbeads (Miltenyi Biotec) as described.3 (link) Protein bound to nanoparticles was measured by fluorescence as described in the Supporting Information.
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3

Nano-aAPCs: MHC-Ig Dimer Conjugation

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Soluble MHC-Ig dimers, Kb-Ig, Db-Ig, and A2-Ig were prepared and loaded with peptides as described,34 (link) see supplementary methods. Nano-aAPC were manufactured by direct conjugation of MHC-Ig dimer and anti-CD28 antibody (37.51; BioLegend) to MACS Microbeads (Miltenyi Biotec) as described previously14 (link).
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