Taqman 48

Manufactured by Roche

The TaqMan 48 is a real-time PCR instrument designed for high-throughput genetic analysis. It has a 48-well format and supports a variety of fluorescent-based detection chemistries, enabling precise quantification of nucleic acid targets.

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Lab products found in correlation

Ampliprep, by Roche (1 mentions) Amplicor version 1, by Roche (1 mentions)

4 protocols using taqman 48

Cohort Study of HIV Viral Load

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Specimens from adult subjects from the following cohorts were used in our study:
HIV plasma viral load was measured by the Roche Amplicor version 1.5 assay with with COBAS AmpliPrep or TaqMan 48 for the Acute HIV Infection cohort or with COBAS Amplicor for the other cohorts. CD4+ T-cell counts were determined by flow cytometry using standard clinical protocol. CD4+ T-cell counts and viral loads were measured on clinical grounds and the data were supplied by the Centre of Recruitment.HLA typing was performed using a locus specific PCR amplification strategy and a heterozygous DNA sequencing methodology for exon 2 and 3 of the class I PCR amplicon [21 (link)].

Leitman E.M., Palmer C.D., Buus S., Chen F., Riddell L., Sims S., Klenerman P., Sáez-Cirión A., Walker B.D., Hess P.R., Altfeld M., Matthews P.C, & Goulder P.J. (2017). Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities. PLoS ONE, 12(10), e0184496.

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Quantification of HBV Markers

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HBsAg, anti-HBe and HBV DNA levels were measured as described in our previous studies [20 (link)]. The detection limit of plasma HBV DNA by the Roche TaqMan48 automatic florescence quantitative polymerase chain reaction (qPCR) kit was 12 IU/mL.

Hao R., Xiang K., Shi Y., Zhao D., Tian H., Xu B., Zhu Y., Dong H., Ding H., Zhuang H., Hu J, & Li T. (2019). Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients. Viruses, 11(1), 78.

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Quantification of HIV Viral Load

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CD4⁺ T lymphocyte cell counts were completed within 24 hours from the collected whole blood samples. The plasma was then separated at 1500 rpm for 15 minutes using a Beckman-Kurt centrifuge. The plasma was packed and frozen at −70°C. The viral load in plasma was determined using Roche TaqMan 48 and matched HIV viral load detection kit. HIV-1 RNA was extracted from plasma using QIAampRNA MiniKit reagent from QIAGEN. Nested reverse transcription fluorescence quantitative polymerase chain reaction amplification was performed on 1.2 KB long fragment of pol region gene (1–99 codons in protease region and 1–250 codons in reverse transcriptase region) by In-house method. The amplified products were identified by agarose gel electrophoresis imaging. The amplified positive products were sent to Beijing Bomeid Gene Technology Co. Ltd. for purification and gene sequencing. Sanger sequencing method was used. Sequencing primers refer to “HIV resistance monitoring strategy and detection technology.”^{[17 ]}

Ma N., Chen X.H., Zhao Y., Kang X., Pan S, & Yao W.Q. (2021). HIV-1 molecular transmission network among sexually transmitted populations in Liaoning Province, China. Medicine, 100(28), e26640.

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Viral Load Quantification and Genotyping

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The collected whole blood samples were counted for CD4 + T lymphocytes (CD4 cells for short) within 24 hours, and then plasma was separated using a Beckman Coulter centrifuge at 1500 rpm for 15 minutes, and the plasma was divided and stored at -70 .Roche's TaqMan48 and the accompanying HIV viral load detection kit were used to determine the viral load in plasma. HIV-1 ribonucleic acid (RNA) was extracted from the plasma using QIAGEN's IAampRNA MiniKit reagent. In-house method was used to perform a nested reverse transcription fluorescent quantitative polymerase chain reaction (RT-PCR) on a 1.2 kb fragment of the pol region gene (1-99 codons in the protease region and 1-250 codons in the reverse transcriptase region). ) Amplification.The amplified products were identified by agarose gel electrophoresis.The amplified positive products were sent to Beijing Bomad Gene Technology Co., Ltd. for purification and gene sequencing, and the sequencing primers refer to "HIV Drug Resistance Surveillance Strategy and Detection Technology" [11] .

Ma N., Chen X., Zhao Y., Xu K., Pan S., & Yao W. (2020). Analysis of HIV-1 Molecular Transmission Network in Sexually Transmitted Population in Liaoning, China.

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