Humulin nph

Manufactured by Eli Lilly

Humulin NPH is a type of insulin that is designed to provide a steady, long-lasting supply of insulin to the body. It is a human insulin product that is manufactured using recombinant DNA technology. Humulin NPH is an intermediate-acting insulin that is used to control blood sugar levels in people with diabetes.

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Lab products found in correlation

3 3 diaminobenzidine tetrahydrochloride dab, by Merck Group (2 mentions) Human chorionic gonadotropin (hcg), by Organon (2 mentions) Alexa fluor 488 conjugated goat polyclonal anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Anti mouse igg horseradish peroxidase hrp conjugated goat a2304, by Merck Group (1 mentions) Anti rabbit igg hrp conjugated goat a0545, by Merck Group (1 mentions) Alexa fluor 594 conjugated goat polyclonal anti mouse igg, by Thermo Fisher Scientific (1 mentions) Avidin biotinylated peroxidase complex detection system abc kit, by Vector Laboratories (1 mentions) D glucose, by Thermo Fisher Scientific (1 mentions) Sesame oil s3547, by Merck Group (1 mentions) Oxiselect in vitro ros rns assay kit, by Cell Biolabs (1 mentions)

Spelling variants of this product

Humulin (192 citations) NPH) Humulin N (2 citations)

4 protocols using humulin nph

Immunohistochemical and Western Blot Analyses

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hCG was from NV Organon (Oss, Holland). Human recombinant insulin (Humulin NPH) was from Eli Lilly (Lilly Pharmaceuticals, Giza, Egypt). 3,3-diaminobenzidine tetrahydrochloride (DAB) was from Sigma-Aldrich (St. Louis, MO). The avidin-biotinylated-peroxidase complex detection system (ABC kit) was from Vector Laboratories Inc. (Burlingame, CA). The primary antibodies used for Western blot and immunohistochemical analyses in the present study, their dilution, and sources are listed in Supplemental Table 1. Anti-mouse IgG horseradish peroxidase (HRP)-conjugated goat (A2304), and anti-rabbit IgG HRP-conjugated goat (A0545) secondary antibodies were from Sigma-Aldrich. Alexa Fluor 594-conjugated goat polyclonal anti-mouse IgG and Alexa Fluor 488-conjugated goat polyclonal anti-rabbit IgG were from Invitrogen (UK).

Zhang Y., Sun X., Sun X., Meng F., Hu M., Li X., Li W., Wu X.K., Brännström M., Shao R, & Billig H. (2016). Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus. Scientific Reports, 6, 30679.

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Glucose and Insulin Tolerance Testing

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Glucose tolerance was determined by i.p. administration of D-glucose (1 g/kg of body weight; A16828; Alfa Aesar) after fasting for 16 h. Insulin tolerance was determined by i.p. injection of Humulin NPH (HI 0319; Lilly; 1 U/kg) after fasting for 5 h. Glucose was measured before and 15, 30, 60, 90, and 120 min after injection in blood drawn from a tail venesection using a handheld glucometer (Contour XT) and strips (Contour Next).

Katsouda A., Valakos D., Dionellis V.S., Bibli S.I., Akoumianakis I., Karaliota S., Zuhra K., Fleming I., Nagahara N., Havaki S., Gorgoulis V.G., Thanos D., Antoniades C., Szabo C, & Papapetropoulos A. (2022). MPST sulfurtransferase maintains mitochondrial protein import and cellular bioenergetics to attenuate obesity. The Journal of Experimental Medicine, 219(7), e20211894.

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Immunostaining Protocol for Protein Analysis

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hCG was from NV Organon (Oss, Holland). Human recombinant insulin (Humulin NPH) was from Eli Lilly Pharmaceuticals (Giza, Egypt). 3,3-diaminobenzidine tetrahydrochloride (DAB) was from Sigma-Aldrich (St. Louis, MO). The avidin-biotinylated-peroxidase complex detection system was from Vector Laboratories Inc. (Burlingame, CA). The primary antibodies used for Western blot and immunohistochemical analyses in the present study, their dilutions, and their sources are listed in Supplementary Table 1. Anti-mouse IgG horseradish peroxidase (HRP)-conjugated goat (A2304), anti-rabbit IgG HRP-conjugated goat (A0545), and anti-goat IgG HRP-conjugated goat (A5420) secondary antibodies were from Sigma-Aldrich.

Zhang Y., Meng F., Sun X., Sun X., Hu M., Cui P., Vestin E., Li X., Li W., Wu X.K., Jansson J.O., Shao L.R, & Billig H. (2018). Hyperandrogenism and insulin resistance contribute to hepatic steatosis and inflammation in female rat liver. Oncotarget, 9(26), 18180-18197.

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Immunohistochemical Analysis of Oxidative Stress

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Human recombinant insulin (Humulin NPH) was from Eli Lilly Pharmaceuticals (Giza, Egypt). DHT, sesame oil (S3547), and periodic acid-Schiff (PAS; no. 395B) staining kits were purchased from Sigma-Aldrich (St. Louis, MO). 3,3-Diaminobenzidine tetrahydrochloride (DAB; no. SK-4100) and the avidin-biotinylated-peroxidase complex detection system for immunohistochemistry were purchased from Vector Laboratories (Burlingame, CA). The OxiSelect In Vitro ROS/RNS assay kit was purchased from Cell Biolabs (San Diego, CA; STA-347). Anti-mouse IgG horseradish peroxidase (HRP)-conjugated horse (no. 7076) and anti-rabbit IgG HRP-conjugated goat (no. 7074) secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA).

Hu M., Zhang Y., Guo X., Jia W., Liu G., Zhang J., Li J., Cui P., Sferruzzi-Perri A.N., Han Y., Wu X., Ma H., Brännström M., Shao L.R, & Billig H. (2019). Hyperandrogenism and insulin resistance induce gravid uterine defects in association with mitochondrial dysfunction and aberrant reactive oxygen species production. American journal of physiology. Endocrinology and metabolism, 316(5).

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