Raman maps were acquired using a Renishaw in Via Raman microscope. The excitation laser was either a 632.8 nm HeNe laser (ThorLabs) for the n-mercaptobutylnitrile and p-mercaptobenzonitrile monolayer experiments or a 660 nm diode laser (Laser Quantum) for the CN− adsorbed to Ag experiments. The acquisition time per spectrum was adjusted with the incident laser power to generate spectra with sufficient signal to noise ratios for analysis. For CN experiments, the scattering between 1940–2348 cm−1 Raman shift was collected; otherwise, the spectral range was 2016–2470 cm−1. Raman spectra for gold nanoparticles on ultraflat gold films were taken using a homebuilt Raman microscope equipped with dark field microscopy (BD objective, Olympus, LMPlanFLN, NA=0.5). A 632.8 nm HeNe laser (Melles Griot) was used to irradiate the sample in a top illumination geometry and a Horiba Jobin Yvon iHR320 spectrometer was used to resolve the Raman scattering. The laser power measured at the sample was 0.75mW, and the acquisition time was 1s. The spectral data was analyzed using MATLAB and an open-source peak-fitting routine.42 Spectra were fit to a Gaussian lineshape 5 times, and fit of the lowest % RMS error was selected.
632.8 nm hene laser
Manufactured by Thorlabs
The 632.8 nm HeNe laser from Thorlabs is a continuous-wave helium-neon laser that produces red light at a wavelength of 632.8 nanometers. This laser is a stable and reliable source of coherent light.
Automatically generated - may contain errors
Lab products found in correlation
Ihr320 spectrometer, by Horiba (2 mentions)
Lmplanfl n, by Olympus (2 mentions)
Invia raman microscope, by Renishaw (2 mentions)
Water immersion objective, by Olympus (1 mentions)
Model ne 1000, by New Era Pump Systems (1 mentions)
Newton 970 emccd camera, by Oxford Instruments (1 mentions)
Bx51 microscope, by Olympus (1 mentions)
5 protocols using 632.8 nm hene laser
1
Raman Spectroscopy of Surface-Adsorbed Species
2
Raman Spectroscopy with Metal Substrates
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For experiments using Ag substrates, a homebuilt Raman setup was used equipped with a 632.8 nm HeNe laser (Thor Labs).11 (link) A laser power of 0.25 mW with an exposure time of 250 ms was used. 100 spectra were collected in series per sample. 3 series were collected per sample. A 40×/0.8 NA water immersion objective from Olympus was used. The Raman scattering was collected through the same objective and directed to an Andor Shamrock 303i spectrograph with an Andor iDus 401 CCD.
For experiments using Au substrates, a homebuilt Raman setup was used equipped with a 785 nm laser (Oxxius).37 (link) The laser was focused onto the samples through a 40x water immersion objective (NA = 0.8, Olympus). The Raman scattering was collected through the same objective and directed to an Isoplane SCT-320 spectrograph with a ProEM 16002 eXcelon 3 CCD detector (Princeton Instruments). Acquisition times of 250 ms were used with a laser power of 0.25 mW. 100 spectra were collected per series, with 3 series collected per sample, and averaged for analysis.
Syringe pumps (Model NE-1000, New Era Pump Systems Inc.) were used to pump all solutions through the flow cell.
For experiments using Au substrates, a homebuilt Raman setup was used equipped with a 785 nm laser (Oxxius).37 (link) The laser was focused onto the samples through a 40x water immersion objective (NA = 0.8, Olympus). The Raman scattering was collected through the same objective and directed to an Isoplane SCT-320 spectrograph with a ProEM 16002 eXcelon 3 CCD detector (Princeton Instruments). Acquisition times of 250 ms were used with a laser power of 0.25 mW. 100 spectra were collected per series, with 3 series collected per sample, and averaged for analysis.
Syringe pumps (Model NE-1000, New Era Pump Systems Inc.) were used to pump all solutions through the flow cell.
3
Raman Microscopy for SERS Analysis
4
Raman Spectroscopy of Surface-Bound Molecules
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Raman maps were acquired using a Renishaw inVia Raman microscope. The excitation laser was either a 632.8 nm HeNe laser (ThorLabs) for the n-mercaptobutylnitrile and p-mercaptobenzonitrile monolayer experiments or a 660 nm diode laser (Laser Quantum) for the CN– adsorbed to Ag experiments. The acquisition time per spectrum was adjusted with the incident laser power to generate spectra with sufficient signal to noise ratios for analysis. For CN– experiments, the scattering between 1940–2348 cm–1 Raman shift was collected; otherwise, the spectral range was 2016–2470 cm–1. Raman spectra for gold nanoparticles on ultraflat gold films were taken using a homebuilt Raman microscope equipped with dark field microscopy (BD objective, Olympus, LMPlanFLN, NA = 0.5). A 632.8 nm HeNe laser (Melles Griot) was used to irradiate the sample in a top illumination geometry and a Horiba Jobin Yvon iHR320 spectrometer was used to resolve the Raman scattering. The laser power measured at the sample was 0.75 mW, and the acquisition time was 1 s. The spectral data was analyzed using MATLAB and an open-source peak-fitting routine.42 Spectra were fit to a Gaussian lineshape 5 times, and the fit with the lowest % RMS error was selected.
5
Raman Spectroscopic Characterization of Samples
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