The cellular ATP levels were measured using a firefly luciferase-based ATP assay kit (Beyotime) based on a fluorescence technique (Genmed Scientifics Inc.) as previously described (31 (link)). The JC-1 kit (Beyotime) was applied to assess changes in the mitochondrial membrane potential (ΔΨm). The mPTP opening was observed as a rapid dissipation of tetramethylrhodamine ethyl ester (TMRE) fluorescence according to a previous study (28 (link)).
Fluorescence technique
Manufactured by Genmed Scientifics
Fluorescence technique is a method used to detect and quantify the presence of specific molecules or compounds in a sample. It involves the excitation of fluorescent molecules, which then emit light at a longer wavelength. This technique is widely used in various scientific and medical applications for analysis, detection, and imaging purposes.
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Lab products found in correlation
2 protocols using fluorescence technique
1
Measuring Cellular ATP and Mitochondrial Function
2
Quantifying Cardiomyocyte Death and Mitochondrial Function
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The TUNEL assay was used to detect cellular death following the IR injury. For the quantification, the numbers of TUNEL-positive cells in the infarcted areas or cardiomyocytes were calculated by counting at least 5 random separate fields as the ratio of the experimental samples to the control samples. The opening of the mPTP was observed as a rapid dissipation of tetramethylrhodamine ethyl ester fluorescence according to our previous studies. The cellular ATP levels were measured using a firefly luciferase-based ATP assay kit (Beyotime) based on a fluorescence technique (Genmed Scientifics Inc.) as previously described [25] (link).
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