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Goat anti chicken igy alexa flour plus 647

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Goat anti-chicken IgY Alexa Flour Plus 647 is a secondary antibody conjugated with Alexa Fluor Plus 647 dye. It is used to detect and quantify chicken immunoglobulin Y (IgY) in various immunoassays and research applications.

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Lab products found in correlation

Goat anti rabbit igg alexa flour 488, by Abcam (3 mentions) Trueview autofluorescence quenching kit, by Vector Laboratories (3 mentions) Sp8 sted 3x confocal microscope, by Leica (2 mentions) Goat anti mouse igg alexa fluor plus 647, by Thermo Fisher Scientific (2 mentions) Imaris, by Oxford Instruments (2 mentions) Sp8 sted 3 microscope, by Leica (1 mentions)

Close spelling variants of this product

Alexa 647 (424 citations) Alexa Flour 647 (46 citations) PLUSTM (3 citations) Goat anti-chicken IgY (2 citations) Alexa Flours (2 citations)

3 protocols using goat anti chicken igy alexa flour plus 647

1

Immunofluorescence Imaging of Cellular Proteins

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Specimen slides were prepared as before. Following overnight incubation with primary antibodies, samples were incubated with two secondary antibodies (goat anti-chicken IgY Alexa Flour Plus 647 [Thermo Fischer Scientific, Waltham, MA] and goat anti-rabbit IgG Alexa Flour 488 [Abcam, Cambridge MA]), rinsed, and then stained with DAPI using the TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories, Burlingame, CA) as outlined by the manufacturer. Images were captured using a Leica SP8 STED 3 × microscope (Leica Microsystems Inc., Buffalo Grove, IL). Acquired Z-stacks were further processed to generate figure images using Fiji ImageJ software (version 1.0, https://imagej.net/Fiji)57 (link).
Nonnecke E.B., Castillo P.A., Dugan A.E., Almalki F., Underwood M.A., De La Motte C.A., Yuan W., Lu W., Shen B., Johansson M.E., Kiessling L.L., Hollox E.J., Lönnerdal B, & Bevins C.L. (2021). Human intelectin-1 (ITLN1) genetic variation and intestinal expression. Scientific Reports, 11, 12889.
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2

Multi-Fluorescent Immunohistochemistry Protocol

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Specimen slides were prepared as described above. Tissues were incubated overnight at 4°C with primary antibodies. After washing in PBS, specimens were incubated with a mixture of secondary antibodies (goat anti‐chicken IgY Alexa Flour Plus 647 [Thermo Fischer Scientific, Waltham, MA; #A32933], goat anti‐rabbit IgG Alexa Flour 488 [Abcam, Cambridge MA; #ab150077] and/or goat anti‐mouse IgG Alexa Fluor Plus 647 [Thermo Fischer Scientific, Waltham, MA; #A32728]) for 1–2 h at room temperature. The slides were then rinsed and stained with DAPI using the TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories, Burlingame, CA; #SP‐8400‐15) according to the manufacturer's protocol. Images were captured using a Leica SP8 STED 3X confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL). Acquired Z‐stacks and figure images were generated using Imaris (Oxford Instruments, Zurich, CH) and Fiji ImageJ (version 1.0, https://imagej.net/Fiji) software programs.44
Nonnecke E.B., Castillo P.A., Johansson M.E., Hollox E.J., Shen B., Lönnerdal B, & Bevins C.L. (2022). Human intelectin‐2 (ITLN2) is selectively expressed by secretory Paneth cells. The FASEB Journal, 36(3), e22200.
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3

Immunofluorescence Staining of Cell Samples

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Specimen slides were prepared as described above. Tissues were incubated overnight at 4°C with primary antibodies. After washing in PBS, specimens were incubated with a mixture of secondary antibodies (goat anti-chicken IgY Alexa Flour Plus 647 [Thermo Fischer Scientific, Waltham, MA; #A32933], goat anti-rabbit IgG Alexa Flour 488 [Abcam, Cambridge MA; #ab150077] and/or goat anti-mouse IgG Alexa Fluor Plus 647 [Thermo Fischer Scientific, Waltham, MA; #A32728]) for 1–2 h at room temperature. The slides were then rinsed and stained with DAPI using the TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories, Burlingame, CA; #SP-8400–15) according to the manufacturer’s protocol. Images were captured using a Leica SP8 STED 3X confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL). Acquired Z-stacks and figure images were generated using Imaris (Oxford Instruments, Zurich, CH) and Fiji ImageJ (version 1.0, https://imagej.net/Fiji) software programs (44 (link)).
Nonnecke E.B., Castillo P.A., Johansson M.E., Hollox E.J., Shen B., Lönnerdal B, & Bevins C.L. (2022). Human intelectin‐2 (ITLN2) is selectively expressed by secretory Paneth cells. The FASEB Journal, 36(3), e22200.
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