Sections of tumor microarray and breast cancer tissue resected from mice were deparaffinized and rehydrated in routine series. Antigen retrieval was performed with IHC-Tek epitope retrieval solution (IHC World, Woodstock, MD, USA) in a humidified-heated chamber. Sections were incubated overnight at 4 °C with antibodies to SPIN90 (lab-made), α-SMA (CST), Vimentin (clone V9, sc-6260, Santa Cruz, Dallas, TX, USA), and E-cadherin (clone G10, sc-88426, Santa Cruz) overnight at 4 °C. The nuclei were counterstained with DAPI or Mayer’s hematoxylin (Dako, Santa Clara, USA), and the stained area was observed using a confocal microscope (FV1000; Olympus, Tokyo, Japan), research slide scanner (Olympus), or Aperio ImageScope (Leica, Wetzlar, Germany).
Research slide scanner
Manufactured by Olympus
Sourced in Japan
The Olympus research slide scanner is a high-performance digital imaging device designed for scientific and medical applications. It captures high-resolution images of microscope slides, enabling researchers to digitize their samples for analysis, archiving, and collaborative work.
Automatically generated - may contain errors
Lab products found in correlation
Fv1000, by Olympus (2 mentions)
α sma, by Santa Cruz Biotechnology (1 mentions)
Aperio imagescope, by Leica (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Agilent Technologies (1 mentions)
Ihc tek epitope retrieval solution, by IHC World (1 mentions)
Mayer s hematoxylin, by Agilent Technologies (1 mentions)
Citrate solution, by Wuhan Servicebio Technology (1 mentions)
Anti dig pod antibody, by Roche (1 mentions)
3 protocols using research slide scanner
1
Immunohistochemical Analysis of Tumor Microenvironment
2
Immunohistochemical Detection of ADAR1
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Paraffin-embedded liver tissue was dewaxed and rehydrated followed by antigen retrieval in a citrate solution (pH 6.0) (Servicebio). After blocking to prevent nonspecific protein binding, the sections were incubated with an anti-ADAR1 primary antibody (#SC-73408; Santa Cruz Biotechnology, Dallas, TX, USA) in a wet box at 4°C overnight. The sections were then incubated with horseradish peroxidase-conjugated secondary antibody. A diaminobenzidine (DAB) chromogenic kit (#G1212; Servicebio) was used for visualization. Hematoxylin was used to counterstain nuclei. The sections were observed with research slide scanner (#VS200; Olympus, Tokyo, Japan) after mounting. The mean number of positively stained cells and the density of the chromogen staining (% area) were quantified with ImageJ software.
3
In-situ detection of miR-130b-3p
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Deparaffinized and rehydrated tissue sections were treated with 3% H2O2 (Sigma-Aldrich, St. Louis, MO, USA) to inhibit intrinsic peroxidase activity. After antigen retrieval in a heated chamber, the sections were incubated with locked nucleic acid (LNA)-modified oligonucleotide probe for hsa-miR-130b-3p (QIAGEN) at 54 °C, for 1 h. Immediately after, a stringent wash was performed in 54 °C-heated SSC buffer (Sigma-Aldrich) according to the manufacturer’s protocol. Samples were incubated with a 1:400 dilution of anti-DIG-POD antibody (11207733910, Roche), and treated with TSA-plus Cy3 amplification solution (Akoya Biosciences, Marlborough, MA, USA). Amplified fluorescence signals were detected by confocal microscopy (FV1000), or research slide scanner (Olympus).
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